In vitro generation of lymphohematopoietic cells from endothelial cells purified from murine embryos

Shin Ichi Nishikawa; Satomi Nishikawa; Hiroshi Kawamoto; Hisahiro Yoshida; Masami Kizumoto; Hiroshi Kataoka; Yoshimoto Katsura

doi:10.1016/S1074-7613(00)80581-6

In vitro generation of lymphohematopoietic cells from endothelial cells purified from murine embryos

Shin Ichi Nishikawa
, Satomi Nishikawa
, Hiroshi Kawamoto
, Hisahiro Yoshida
, Masami Kizumoto
, Hiroshi Kataoka
, Yoshimoto Katsura

International Center for Cell and Gene Therapy

Research output: Contribution to journal › Article › peer-review

314 Citations (Scopus)

Abstract

We have investigated the lymphohematopoietic potentials of endothelial cells (EC) and hematopoietic cells (HPC) sorted from embryos. Expression of VE-cadherin, CD45, and Ter119 was used to distinguish EC (VE- cadherin⁺CD45^-Ter119^-) from HPC (VE-cadherin^-CD45⁺). Thus defined, EC population takes up acetylated LDL and coexpresses CD31, Flk1, and CD34. In E9.5 embryos, EC from yolk sac (YS) and the embryo proper generate blood cells, including lymphocytes. Thus, lymphohematopoietic EC do exist in the embryo, and they are generated both in YS and the embryo proper. On the other hand, HPC with lymphopoietic potency appear first in the embryo proper. These findings implicate involvement of multiple environmental cues for acquiring lymphopoietic competency during differentiation of HPC.

Original language	English
Pages (from-to)	761-769
Number of pages	9
Journal	Immunity
Volume	8
Issue number	6
DOIs	https://doi.org/10.1016/S1074-7613(00)80581-6
Publication status	Published - 06-1998

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

All Science Journal Classification (ASJC) codes

Immunology and Allergy
Immunology
Infectious Diseases

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10.1016/S1074-7613(00)80581-6

Cite this

@article{1ff44e34bb7b4de981e3831fbba60e4c,

title = "In vitro generation of lymphohematopoietic cells from endothelial cells purified from murine embryos",

abstract = "We have investigated the lymphohematopoietic potentials of endothelial cells (EC) and hematopoietic cells (HPC) sorted from embryos. Expression of VE-cadherin, CD45, and Ter119 was used to distinguish EC (VE- cadherin+CD45-Ter119-) from HPC (VE-cadherin-CD45+). Thus defined, EC population takes up acetylated LDL and coexpresses CD31, Flk1, and CD34. In E9.5 embryos, EC from yolk sac (YS) and the embryo proper generate blood cells, including lymphocytes. Thus, lymphohematopoietic EC do exist in the embryo, and they are generated both in YS and the embryo proper. On the other hand, HPC with lymphopoietic potency appear first in the embryo proper. These findings implicate involvement of multiple environmental cues for acquiring lymphopoietic competency during differentiation of HPC.",

author = "Nishikawa, \{Shin Ichi\} and Satomi Nishikawa and Hiroshi Kawamoto and Hisahiro Yoshida and Masami Kizumoto and Hiroshi Kataoka and Yoshimoto Katsura",

note = "Funding Information: We thank N. Matsuyoshi for VECD1 mAb and L. Zon and S. Fraser for critical reading of the manuscript. This study is supported by grants from the Ministry of Education, Science, Sports, and Culture of Japan (No. 07CE2005, 06277102, 06NP1101).",

year = "1998",

month = jun,

doi = "10.1016/S1074-7613(00)80581-6",

language = "English",

volume = "8",

pages = "761--769",

journal = "Immunity",

issn = "1074-7613",

publisher = "Cell Press",

number = "6",

}

TY - JOUR

T1 - In vitro generation of lymphohematopoietic cells from endothelial cells purified from murine embryos

AU - Nishikawa, Shin Ichi

AU - Nishikawa, Satomi

AU - Kawamoto, Hiroshi

AU - Yoshida, Hisahiro

AU - Kizumoto, Masami

AU - Kataoka, Hiroshi

AU - Katsura, Yoshimoto

N1 - Funding Information: We thank N. Matsuyoshi for VECD1 mAb and L. Zon and S. Fraser for critical reading of the manuscript. This study is supported by grants from the Ministry of Education, Science, Sports, and Culture of Japan (No. 07CE2005, 06277102, 06NP1101).

PY - 1998/6

Y1 - 1998/6

N2 - We have investigated the lymphohematopoietic potentials of endothelial cells (EC) and hematopoietic cells (HPC) sorted from embryos. Expression of VE-cadherin, CD45, and Ter119 was used to distinguish EC (VE- cadherin+CD45-Ter119-) from HPC (VE-cadherin-CD45+). Thus defined, EC population takes up acetylated LDL and coexpresses CD31, Flk1, and CD34. In E9.5 embryos, EC from yolk sac (YS) and the embryo proper generate blood cells, including lymphocytes. Thus, lymphohematopoietic EC do exist in the embryo, and they are generated both in YS and the embryo proper. On the other hand, HPC with lymphopoietic potency appear first in the embryo proper. These findings implicate involvement of multiple environmental cues for acquiring lymphopoietic competency during differentiation of HPC.

AB - We have investigated the lymphohematopoietic potentials of endothelial cells (EC) and hematopoietic cells (HPC) sorted from embryos. Expression of VE-cadherin, CD45, and Ter119 was used to distinguish EC (VE- cadherin+CD45-Ter119-) from HPC (VE-cadherin-CD45+). Thus defined, EC population takes up acetylated LDL and coexpresses CD31, Flk1, and CD34. In E9.5 embryos, EC from yolk sac (YS) and the embryo proper generate blood cells, including lymphocytes. Thus, lymphohematopoietic EC do exist in the embryo, and they are generated both in YS and the embryo proper. On the other hand, HPC with lymphopoietic potency appear first in the embryo proper. These findings implicate involvement of multiple environmental cues for acquiring lymphopoietic competency during differentiation of HPC.

UR - https://www.scopus.com/pages/publications/17744416048

UR - https://www.scopus.com/pages/publications/17744416048#tab=citedBy

U2 - 10.1016/S1074-7613(00)80581-6

DO - 10.1016/S1074-7613(00)80581-6

M3 - Article

C2 - 9655490

AN - SCOPUS:17744416048

SN - 1074-7613

VL - 8

SP - 761

EP - 769

JO - Immunity

JF - Immunity

IS - 6

ER -

In vitro generation of lymphohematopoietic cells from endothelial cells purified from murine embryos

Abstract

UN SDGs

All Science Journal Classification (ASJC) codes

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