In vitro organogenesis from undifferentiated cells in Xenopus

Makoto Asashima, Yuzuru Ito, Techuan Chan, Tatsuo Michiue, Mio Nakanishi, Kan Suzuki, Keisuke Hitachi, Koji Okabayashi, Akiko Kondo, Takashi Ariizumi

Research output: Contribution to journalReview article

25 Citations (Scopus)

Abstract

Amphibians have been used for over a century as experimental animals. In the field of developmental biology in particular, much knowledge has been accumulated from studies on amphibians, mainly because they are easy to observe and handle. Xenopus laevis is one of the most intensely investigated amphibians in developmental biology at the molecular level. Thus, Xenopus is highly suitable for studies on the mechanisms of organ differentiation from not only a single fertilized egg, as in normal development, but also from undifferentiated cells, as in the case of in vitro organogenesis. Based on the established in vitro organogenesis methods, we have identified many genes that are indispensable for normal development in various organs. These experimental systems are useful for investigations of embryonic development and for advancing regenerative medicine.

Original languageEnglish
Pages (from-to)1309-1320
Number of pages12
JournalDevelopmental Dynamics
Volume238
Issue number6
DOIs
Publication statusPublished - 01-06-2009

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Organogenesis
Amphibians
Xenopus
Developmental Biology
Regenerative Medicine
Zygote
Xenopus laevis
Embryonic Development
Genes
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Developmental Biology

Cite this

Asashima, M., Ito, Y., Chan, T., Michiue, T., Nakanishi, M., Suzuki, K., ... Ariizumi, T. (2009). In vitro organogenesis from undifferentiated cells in Xenopus. Developmental Dynamics, 238(6), 1309-1320. https://doi.org/10.1002/dvdy.21979
Asashima, Makoto ; Ito, Yuzuru ; Chan, Techuan ; Michiue, Tatsuo ; Nakanishi, Mio ; Suzuki, Kan ; Hitachi, Keisuke ; Okabayashi, Koji ; Kondo, Akiko ; Ariizumi, Takashi. / In vitro organogenesis from undifferentiated cells in Xenopus. In: Developmental Dynamics. 2009 ; Vol. 238, No. 6. pp. 1309-1320.
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Asashima, M, Ito, Y, Chan, T, Michiue, T, Nakanishi, M, Suzuki, K, Hitachi, K, Okabayashi, K, Kondo, A & Ariizumi, T 2009, 'In vitro organogenesis from undifferentiated cells in Xenopus', Developmental Dynamics, vol. 238, no. 6, pp. 1309-1320. https://doi.org/10.1002/dvdy.21979

In vitro organogenesis from undifferentiated cells in Xenopus. / Asashima, Makoto; Ito, Yuzuru; Chan, Techuan; Michiue, Tatsuo; Nakanishi, Mio; Suzuki, Kan; Hitachi, Keisuke; Okabayashi, Koji; Kondo, Akiko; Ariizumi, Takashi.

In: Developmental Dynamics, Vol. 238, No. 6, 01.06.2009, p. 1309-1320.

Research output: Contribution to journalReview article

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Asashima M, Ito Y, Chan T, Michiue T, Nakanishi M, Suzuki K et al. In vitro organogenesis from undifferentiated cells in Xenopus. Developmental Dynamics. 2009 Jun 1;238(6):1309-1320. https://doi.org/10.1002/dvdy.21979