TY - JOUR
T1 - In vitro organogenesis from undifferentiated cells in Xenopus
AU - Asashima, Makoto
AU - Ito, Yuzuru
AU - Chan, Techuan
AU - Michiue, Tatsuo
AU - Nakanishi, Mio
AU - Suzuki, Kan
AU - Hitachi, Keisuke
AU - Okabayashi, Koji
AU - Kondow, Akiko
AU - Ariizumi, Takashi
N1 - Copyright:
Copyright 2009 Elsevier B.V., All rights reserved.
PY - 2009/6
Y1 - 2009/6
N2 - Amphibians have been used for over a century as experimental animals. In the field of developmental biology in particular, much knowledge has been accumulated from studies on amphibians, mainly because they are easy to observe and handle. Xenopus laevis is one of the most intensely investigated amphibians in developmental biology at the molecular level. Thus, Xenopus is highly suitable for studies on the mechanisms of organ differentiation from not only a single fertilized egg, as in normal development, but also from undifferentiated cells, as in the case of in vitro organogenesis. Based on the established in vitro organogenesis methods, we have identified many genes that are indispensable for normal development in various organs. These experimental systems are useful for investigations of embryonic development and for advancing regenerative medicine.
AB - Amphibians have been used for over a century as experimental animals. In the field of developmental biology in particular, much knowledge has been accumulated from studies on amphibians, mainly because they are easy to observe and handle. Xenopus laevis is one of the most intensely investigated amphibians in developmental biology at the molecular level. Thus, Xenopus is highly suitable for studies on the mechanisms of organ differentiation from not only a single fertilized egg, as in normal development, but also from undifferentiated cells, as in the case of in vitro organogenesis. Based on the established in vitro organogenesis methods, we have identified many genes that are indispensable for normal development in various organs. These experimental systems are useful for investigations of embryonic development and for advancing regenerative medicine.
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U2 - 10.1002/dvdy.21979
DO - 10.1002/dvdy.21979
M3 - Review article
C2 - 19441056
AN - SCOPUS:66349096033
VL - 238
SP - 1309
EP - 1320
JO - Developmental Dynamics
JF - Developmental Dynamics
SN - 1058-8388
IS - 6
ER -