In vitro study of endotoxin adsorption by a polymyxin b-immobilized fiber column

Chizuru Yamashita, Kazuhiro Moriyama, Daisuke Hasegawa, Yuu Toki, Toshikazu Sakai, Takahiro Kawaji, Yasuyo Shimomura, Yasuyoshi Kurimoto, Mariko Nagata, Osamu Nishida

Research output: Contribution to journalArticle

Abstract

Background: Polymyxin B-immobilized fiber (PMX-F) columns are used as therapeutic interventions for septic shock. The clinical efficacy has been reported for 2-h applications, but their ability to adsorb endotoxin over longer treatments has not been fully elucidated. We hypothesized that PMX-F columns are capable of endotoxin removal for more than 2 h. Method: We designed closed circuits incorporating either a PMX-F column with an 8.5-mL priming volume (PMX-01R) or a sham-control column, and used inactivated fetal bovine serum as the circulating perfusate. Endotoxin was continuously injected at a fixed rate for 24 h, and perfusate endotoxin concentrations were measured at fixed time points. PMX-01R endotoxin adsorption was calculated from the difference in the endotoxin concentrations. Results: PMX-01R endotoxin adsorption increased continuously in a virtually linear manner. Conclusions: The PMX-01R column showed sustained endotoxin adsorption for at least 24 h. This indicated that PMX-F columns would be capable of clinical endotoxin removal for 24 h.

Original languageEnglish
Pages (from-to)269-273
Number of pages5
JournalBlood Purification
Volume46
Issue number4
DOIs
Publication statusPublished - 01-10-2018

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Polymyxins
Endotoxins
Adsorption
Polymyxin B
In Vitro Techniques
Septic Shock

All Science Journal Classification (ASJC) codes

  • Hematology
  • Nephrology

Cite this

Yamashita, Chizuru ; Moriyama, Kazuhiro ; Hasegawa, Daisuke ; Toki, Yuu ; Sakai, Toshikazu ; Kawaji, Takahiro ; Shimomura, Yasuyo ; Kurimoto, Yasuyoshi ; Nagata, Mariko ; Nishida, Osamu. / In vitro study of endotoxin adsorption by a polymyxin b-immobilized fiber column. In: Blood Purification. 2018 ; Vol. 46, No. 4. pp. 269-273.
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abstract = "Background: Polymyxin B-immobilized fiber (PMX-F) columns are used as therapeutic interventions for septic shock. The clinical efficacy has been reported for 2-h applications, but their ability to adsorb endotoxin over longer treatments has not been fully elucidated. We hypothesized that PMX-F columns are capable of endotoxin removal for more than 2 h. Method: We designed closed circuits incorporating either a PMX-F column with an 8.5-mL priming volume (PMX-01R) or a sham-control column, and used inactivated fetal bovine serum as the circulating perfusate. Endotoxin was continuously injected at a fixed rate for 24 h, and perfusate endotoxin concentrations were measured at fixed time points. PMX-01R endotoxin adsorption was calculated from the difference in the endotoxin concentrations. Results: PMX-01R endotoxin adsorption increased continuously in a virtually linear manner. Conclusions: The PMX-01R column showed sustained endotoxin adsorption for at least 24 h. This indicated that PMX-F columns would be capable of clinical endotoxin removal for 24 h.",
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Yamashita, C, Moriyama, K, Hasegawa, D, Toki, Y, Sakai, T, Kawaji, T, Shimomura, Y, Kurimoto, Y, Nagata, M & Nishida, O 2018, 'In vitro study of endotoxin adsorption by a polymyxin b-immobilized fiber column', Blood Purification, vol. 46, no. 4, pp. 269-273. https://doi.org/10.1159/000489920

In vitro study of endotoxin adsorption by a polymyxin b-immobilized fiber column. / Yamashita, Chizuru; Moriyama, Kazuhiro; Hasegawa, Daisuke; Toki, Yuu; Sakai, Toshikazu; Kawaji, Takahiro; Shimomura, Yasuyo; Kurimoto, Yasuyoshi; Nagata, Mariko; Nishida, Osamu.

In: Blood Purification, Vol. 46, No. 4, 01.10.2018, p. 269-273.

Research output: Contribution to journalArticle

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T1 - In vitro study of endotoxin adsorption by a polymyxin b-immobilized fiber column

AU - Yamashita, Chizuru

AU - Moriyama, Kazuhiro

AU - Hasegawa, Daisuke

AU - Toki, Yuu

AU - Sakai, Toshikazu

AU - Kawaji, Takahiro

AU - Shimomura, Yasuyo

AU - Kurimoto, Yasuyoshi

AU - Nagata, Mariko

AU - Nishida, Osamu

PY - 2018/10/1

Y1 - 2018/10/1

N2 - Background: Polymyxin B-immobilized fiber (PMX-F) columns are used as therapeutic interventions for septic shock. The clinical efficacy has been reported for 2-h applications, but their ability to adsorb endotoxin over longer treatments has not been fully elucidated. We hypothesized that PMX-F columns are capable of endotoxin removal for more than 2 h. Method: We designed closed circuits incorporating either a PMX-F column with an 8.5-mL priming volume (PMX-01R) or a sham-control column, and used inactivated fetal bovine serum as the circulating perfusate. Endotoxin was continuously injected at a fixed rate for 24 h, and perfusate endotoxin concentrations were measured at fixed time points. PMX-01R endotoxin adsorption was calculated from the difference in the endotoxin concentrations. Results: PMX-01R endotoxin adsorption increased continuously in a virtually linear manner. Conclusions: The PMX-01R column showed sustained endotoxin adsorption for at least 24 h. This indicated that PMX-F columns would be capable of clinical endotoxin removal for 24 h.

AB - Background: Polymyxin B-immobilized fiber (PMX-F) columns are used as therapeutic interventions for septic shock. The clinical efficacy has been reported for 2-h applications, but their ability to adsorb endotoxin over longer treatments has not been fully elucidated. We hypothesized that PMX-F columns are capable of endotoxin removal for more than 2 h. Method: We designed closed circuits incorporating either a PMX-F column with an 8.5-mL priming volume (PMX-01R) or a sham-control column, and used inactivated fetal bovine serum as the circulating perfusate. Endotoxin was continuously injected at a fixed rate for 24 h, and perfusate endotoxin concentrations were measured at fixed time points. PMX-01R endotoxin adsorption was calculated from the difference in the endotoxin concentrations. Results: PMX-01R endotoxin adsorption increased continuously in a virtually linear manner. Conclusions: The PMX-01R column showed sustained endotoxin adsorption for at least 24 h. This indicated that PMX-F columns would be capable of clinical endotoxin removal for 24 h.

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