In vivo evaluation of cellular activity in αCaMKII heterozygous knockout mice using manganese-enhanced magnetic resonance imaging (MEMRI)

Satoko Hattori, Hideo Hagihara, Koji Ohira, Ichio Aoki, Tsuneo Saga, Tetsuya Suhara, Makoto Higuchi, Tsuyoshi Miyakawa

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Abstract

The alpha-calcium/calmodulin-dependent protein kinase II (αCaMKII) is a serine/threonine protein kinase predominantly expressed in the forebrain, especially in the postsynaptic density, and plays a key role in synaptic plasticity, learning and memory. αCaMKII heterozygous knockout (HKO) mice exhibit abnormal emotional and aggressive behaviors and cognitive impairments and have been proposed as an animal model of psychiatric illness. Our previous studies have shown that the expression of immediate early genes (IEGs) after exposure to electric foot shock or after performing a working memory task is decreased in the hippocampus, central amygdala, and medial prefrontal cortex of mutant mice. These changes could be caused by disturbances in neuronal signal transduction; however, it is still unclear whether neuronal activity is reduced in these regions. In this study, we performed in vivo manganese-enhanced magnetic resonance imaging (MEMRI) to assess the regional cellular activity in the brains of αCaMKII HKO mice. The signal intensity of MEMRI 24 h after systemic MnCl2 administration reflects functional increases of Mn2+ influx into neurons and glia via transport mechanisms, such as voltage-gated and/or ligand-gated Ca2+ channels. α CaMKII HKO mice demonstrated a low signal intensity of MEMRI in the dentate gyrus (DG), in which almost all neurons were at immature status at the molecular, morphological, and electrophysiological levels. In contrast, analysis of the signal intensity in these mutant mice revealed increased activity in the CA1 area of the hippocampus, a region crucial for cognitive function. The signal intensity was also increased in the bed nucleus of the stria terminalis (BNST), which is involved in anxiety. These changes in the mutant mice may be responsible for the observed dysregulated behaviors, such as cognitive deficit and abnormal anxiety-like behavior, which are similar to symptoms seen in human psychiatric disorders.

Original languageEnglish
Article number76
JournalFrontiers in Integrative Neuroscience
Volume7
Issue numberNOV
DOIs
Publication statusPublished - 11-11-2013

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Calcium-Calmodulin-Dependent Protein Kinase Type 2
Manganese
Knockout Mice
Magnetic Resonance Imaging
Psychiatry
Hippocampus
Anxiety
Ligand-Gated Ion Channels
Post-Synaptic Density
Neurons
Septal Nuclei
Calcium-Calmodulin-Dependent Protein Kinases
Neuronal Plasticity
Immediate-Early Genes
Protein-Serine-Threonine Kinases
Dentate Gyrus
Prosencephalon
Prefrontal Cortex
Short-Term Memory
Neuroglia

All Science Journal Classification (ASJC) codes

  • Sensory Systems
  • Cognitive Neuroscience
  • Cellular and Molecular Neuroscience

Cite this

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title = "In vivo evaluation of cellular activity in αCaMKII heterozygous knockout mice using manganese-enhanced magnetic resonance imaging (MEMRI)",
abstract = "The alpha-calcium/calmodulin-dependent protein kinase II (αCaMKII) is a serine/threonine protein kinase predominantly expressed in the forebrain, especially in the postsynaptic density, and plays a key role in synaptic plasticity, learning and memory. αCaMKII heterozygous knockout (HKO) mice exhibit abnormal emotional and aggressive behaviors and cognitive impairments and have been proposed as an animal model of psychiatric illness. Our previous studies have shown that the expression of immediate early genes (IEGs) after exposure to electric foot shock or after performing a working memory task is decreased in the hippocampus, central amygdala, and medial prefrontal cortex of mutant mice. These changes could be caused by disturbances in neuronal signal transduction; however, it is still unclear whether neuronal activity is reduced in these regions. In this study, we performed in vivo manganese-enhanced magnetic resonance imaging (MEMRI) to assess the regional cellular activity in the brains of αCaMKII HKO mice. The signal intensity of MEMRI 24 h after systemic MnCl2 administration reflects functional increases of Mn2+ influx into neurons and glia via transport mechanisms, such as voltage-gated and/or ligand-gated Ca2+ channels. α CaMKII HKO mice demonstrated a low signal intensity of MEMRI in the dentate gyrus (DG), in which almost all neurons were at immature status at the molecular, morphological, and electrophysiological levels. In contrast, analysis of the signal intensity in these mutant mice revealed increased activity in the CA1 area of the hippocampus, a region crucial for cognitive function. The signal intensity was also increased in the bed nucleus of the stria terminalis (BNST), which is involved in anxiety. These changes in the mutant mice may be responsible for the observed dysregulated behaviors, such as cognitive deficit and abnormal anxiety-like behavior, which are similar to symptoms seen in human psychiatric disorders.",
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In vivo evaluation of cellular activity in αCaMKII heterozygous knockout mice using manganese-enhanced magnetic resonance imaging (MEMRI). / Hattori, Satoko; Hagihara, Hideo; Ohira, Koji; Aoki, Ichio; Saga, Tsuneo; Suhara, Tetsuya; Higuchi, Makoto; Miyakawa, Tsuyoshi.

In: Frontiers in Integrative Neuroscience, Vol. 7, No. NOV, 76, 11.11.2013.

Research output: Contribution to journalArticle

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AU - Hattori, Satoko

AU - Hagihara, Hideo

AU - Ohira, Koji

AU - Aoki, Ichio

AU - Saga, Tsuneo

AU - Suhara, Tetsuya

AU - Higuchi, Makoto

AU - Miyakawa, Tsuyoshi

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AB - The alpha-calcium/calmodulin-dependent protein kinase II (αCaMKII) is a serine/threonine protein kinase predominantly expressed in the forebrain, especially in the postsynaptic density, and plays a key role in synaptic plasticity, learning and memory. αCaMKII heterozygous knockout (HKO) mice exhibit abnormal emotional and aggressive behaviors and cognitive impairments and have been proposed as an animal model of psychiatric illness. Our previous studies have shown that the expression of immediate early genes (IEGs) after exposure to electric foot shock or after performing a working memory task is decreased in the hippocampus, central amygdala, and medial prefrontal cortex of mutant mice. These changes could be caused by disturbances in neuronal signal transduction; however, it is still unclear whether neuronal activity is reduced in these regions. In this study, we performed in vivo manganese-enhanced magnetic resonance imaging (MEMRI) to assess the regional cellular activity in the brains of αCaMKII HKO mice. The signal intensity of MEMRI 24 h after systemic MnCl2 administration reflects functional increases of Mn2+ influx into neurons and glia via transport mechanisms, such as voltage-gated and/or ligand-gated Ca2+ channels. α CaMKII HKO mice demonstrated a low signal intensity of MEMRI in the dentate gyrus (DG), in which almost all neurons were at immature status at the molecular, morphological, and electrophysiological levels. In contrast, analysis of the signal intensity in these mutant mice revealed increased activity in the CA1 area of the hippocampus, a region crucial for cognitive function. The signal intensity was also increased in the bed nucleus of the stria terminalis (BNST), which is involved in anxiety. These changes in the mutant mice may be responsible for the observed dysregulated behaviors, such as cognitive deficit and abnormal anxiety-like behavior, which are similar to symptoms seen in human psychiatric disorders.

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