In vivo gene transfer to cerebral white matter lesions with a recombinant adenovirus vector

Makoto Masumura, Ryuji Hata, Taichi Uetsuki, Isao Nishimura, Yasuo Nagai, Tohru Sawada

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Ischemic white matter lesions have been reported in rats after bilateral common carotid ligation (BCAL). Previously, comparing normotensive rats (WKY) with spontaneously hypertensive rats (SHR), we too found that sustained moderate ischemia with spontaneous hypertension accelerated the formation of ischemic white matter lesions. In this study, we explored the feasibility of gene therapy for lesioned white matter by means of an adenovirus vector expressing a reporter gene, LacZ. Using sham-operated and hypoperfused SHR as well as sham-operated and hypoperfused WKY, we demonstrated that (i) adenovirus vectors could deliver a foreign gene into oligodendrocytes and astrocytes in the cerebral white matter; (ii) the transduction efficiency was most effective in SHR after BCAL; and (iii) the level of αv-integrin was significantly correlated with adenoviral transduction efficiency.

Original languageEnglish
Pages (from-to)440-444
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume287
Issue number2
DOIs
Publication statusPublished - 21-09-2001

Fingerprint

Gene transfer
Adenoviridae
Rats
Inbred SHR Rats
Genes
Ligation
Inbred WKY Rats
Oligodendroglia
Gene therapy
Reporter Genes
Integrins
Astrocytes
Genetic Therapy
Ischemia
Hypertension
White Matter

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Masumura, Makoto ; Hata, Ryuji ; Uetsuki, Taichi ; Nishimura, Isao ; Nagai, Yasuo ; Sawada, Tohru. / In vivo gene transfer to cerebral white matter lesions with a recombinant adenovirus vector. In: Biochemical and Biophysical Research Communications. 2001 ; Vol. 287, No. 2. pp. 440-444.
@article{ad4bbfaf9a254bd78f859f2b2b23c85e,
title = "In vivo gene transfer to cerebral white matter lesions with a recombinant adenovirus vector",
abstract = "Ischemic white matter lesions have been reported in rats after bilateral common carotid ligation (BCAL). Previously, comparing normotensive rats (WKY) with spontaneously hypertensive rats (SHR), we too found that sustained moderate ischemia with spontaneous hypertension accelerated the formation of ischemic white matter lesions. In this study, we explored the feasibility of gene therapy for lesioned white matter by means of an adenovirus vector expressing a reporter gene, LacZ. Using sham-operated and hypoperfused SHR as well as sham-operated and hypoperfused WKY, we demonstrated that (i) adenovirus vectors could deliver a foreign gene into oligodendrocytes and astrocytes in the cerebral white matter; (ii) the transduction efficiency was most effective in SHR after BCAL; and (iii) the level of αv-integrin was significantly correlated with adenoviral transduction efficiency.",
author = "Makoto Masumura and Ryuji Hata and Taichi Uetsuki and Isao Nishimura and Yasuo Nagai and Tohru Sawada",
year = "2001",
month = "9",
day = "21",
doi = "10.1006/bbrc.2001.5609",
language = "English",
volume = "287",
pages = "440--444",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "2",

}

In vivo gene transfer to cerebral white matter lesions with a recombinant adenovirus vector. / Masumura, Makoto; Hata, Ryuji; Uetsuki, Taichi; Nishimura, Isao; Nagai, Yasuo; Sawada, Tohru.

In: Biochemical and Biophysical Research Communications, Vol. 287, No. 2, 21.09.2001, p. 440-444.

Research output: Contribution to journalArticle

TY - JOUR

T1 - In vivo gene transfer to cerebral white matter lesions with a recombinant adenovirus vector

AU - Masumura, Makoto

AU - Hata, Ryuji

AU - Uetsuki, Taichi

AU - Nishimura, Isao

AU - Nagai, Yasuo

AU - Sawada, Tohru

PY - 2001/9/21

Y1 - 2001/9/21

N2 - Ischemic white matter lesions have been reported in rats after bilateral common carotid ligation (BCAL). Previously, comparing normotensive rats (WKY) with spontaneously hypertensive rats (SHR), we too found that sustained moderate ischemia with spontaneous hypertension accelerated the formation of ischemic white matter lesions. In this study, we explored the feasibility of gene therapy for lesioned white matter by means of an adenovirus vector expressing a reporter gene, LacZ. Using sham-operated and hypoperfused SHR as well as sham-operated and hypoperfused WKY, we demonstrated that (i) adenovirus vectors could deliver a foreign gene into oligodendrocytes and astrocytes in the cerebral white matter; (ii) the transduction efficiency was most effective in SHR after BCAL; and (iii) the level of αv-integrin was significantly correlated with adenoviral transduction efficiency.

AB - Ischemic white matter lesions have been reported in rats after bilateral common carotid ligation (BCAL). Previously, comparing normotensive rats (WKY) with spontaneously hypertensive rats (SHR), we too found that sustained moderate ischemia with spontaneous hypertension accelerated the formation of ischemic white matter lesions. In this study, we explored the feasibility of gene therapy for lesioned white matter by means of an adenovirus vector expressing a reporter gene, LacZ. Using sham-operated and hypoperfused SHR as well as sham-operated and hypoperfused WKY, we demonstrated that (i) adenovirus vectors could deliver a foreign gene into oligodendrocytes and astrocytes in the cerebral white matter; (ii) the transduction efficiency was most effective in SHR after BCAL; and (iii) the level of αv-integrin was significantly correlated with adenoviral transduction efficiency.

UR - http://www.scopus.com/inward/record.url?scp=0035929357&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035929357&partnerID=8YFLogxK

U2 - 10.1006/bbrc.2001.5609

DO - 10.1006/bbrc.2001.5609

M3 - Article

C2 - 11554748

AN - SCOPUS:0035929357

VL - 287

SP - 440

EP - 444

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 2

ER -