In vivo visual reporter system for detection of estrogen-like substances by transgenic medaka

Kanta Kurauchi, Yoshitsugu Nakaguchi, Makiko Tsutsumi, Hirosi Hori, Ryo Kurihara, Shinya Hashimoto, Ryoko Ohnuma, Yoshikazu Yamamoto, Sumiko Matsuoka, Shin'Ichiro Kawai, Takashi Hirata, Masato Kinoshita

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Detection of endocrine disrupting chemicals, in particular, environmental estrogens with living organisms, has many advantages if compared to chemical analysis. The screening of novel pollutants with meaningful endpoints, the integration of uptake, bioconcentration, and excretion as well as the evaluation of endocrine disrupting effects with respect to toxicity require in vivo biotests for estrogen-like substances (ELSs). Critical disadvantages of whole organism biotests are their low sensitivity and the need for laborious and time-consuming work. To overcome these problems, we have developed a transgenic medaka strain harboring the green fluorescence protein (GFP) gene driven by choriogenin H gene regulatory elements. Choriogenin H is an egg envelope protein induced by estrogens in the liver. With yolk sac larvae of this strain, GFP induction in liver was observed 24 h after onset of aqueous exposure to 0.63 nM 17β-estradiol (E2), 0.34 nM ethynylestradiol, or 14.8 nM estrone. Furthermore, concentrated sewage treatment effluent induced GFP expression. Comparison of E2 equivalents estimated by GFP-induction in transgenic medaka, a YES assay, and GC/MS showed detection limits in the same order of magnitude. These results indicated that the sensitivity of the transgenic medaka strain was sufficient for application as an alternative model in monitoring environmental water samples for ELSs.

Original languageEnglish
Pages (from-to)2762-2768
Number of pages7
JournalEnvironmental Science and Technology
Volume39
Issue number8
DOIs
Publication statusPublished - 15-04-2005

Fingerprint

Estrogens
fluorescence
Fluorescence
protein
Proteins
Liver
Endocrine Disruptors
Sewage treatment
Estrone
gene
environmental monitoring
sewage treatment
excretion
chemical analysis
bioaccumulation
Toxicity
Effluents
Estradiol
Assays
Screening

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Environmental Chemistry

Cite this

Kurauchi, Kanta ; Nakaguchi, Yoshitsugu ; Tsutsumi, Makiko ; Hori, Hirosi ; Kurihara, Ryo ; Hashimoto, Shinya ; Ohnuma, Ryoko ; Yamamoto, Yoshikazu ; Matsuoka, Sumiko ; Kawai, Shin'Ichiro ; Hirata, Takashi ; Kinoshita, Masato. / In vivo visual reporter system for detection of estrogen-like substances by transgenic medaka. In: Environmental Science and Technology. 2005 ; Vol. 39, No. 8. pp. 2762-2768.
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Kurauchi, K, Nakaguchi, Y, Tsutsumi, M, Hori, H, Kurihara, R, Hashimoto, S, Ohnuma, R, Yamamoto, Y, Matsuoka, S, Kawai, SI, Hirata, T & Kinoshita, M 2005, 'In vivo visual reporter system for detection of estrogen-like substances by transgenic medaka', Environmental Science and Technology, vol. 39, no. 8, pp. 2762-2768. https://doi.org/10.1021/es0486465

In vivo visual reporter system for detection of estrogen-like substances by transgenic medaka. / Kurauchi, Kanta; Nakaguchi, Yoshitsugu; Tsutsumi, Makiko; Hori, Hirosi; Kurihara, Ryo; Hashimoto, Shinya; Ohnuma, Ryoko; Yamamoto, Yoshikazu; Matsuoka, Sumiko; Kawai, Shin'Ichiro; Hirata, Takashi; Kinoshita, Masato.

In: Environmental Science and Technology, Vol. 39, No. 8, 15.04.2005, p. 2762-2768.

Research output: Contribution to journalArticle

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AU - Kurauchi, Kanta

AU - Nakaguchi, Yoshitsugu

AU - Tsutsumi, Makiko

AU - Hori, Hirosi

AU - Kurihara, Ryo

AU - Hashimoto, Shinya

AU - Ohnuma, Ryoko

AU - Yamamoto, Yoshikazu

AU - Matsuoka, Sumiko

AU - Kawai, Shin'Ichiro

AU - Hirata, Takashi

AU - Kinoshita, Masato

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