In vivo visualization of reactive gliosis using manganese-enhanced magnetic resonance imaging

Yuko Kawai, Ichio Aoki, Masahiro Umeda, Toshihiro Higuchi, Jeff Kershaw, Makoto Higuchi, Afonso C. Silva, Chuzo Tanaka

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

Reactive astrogliosis occurs after diverse central nervous system (CNS) insults. While astrogliosis provides protection against inflammation, it is also obstructive in the progress of neuranagenesis after CNS insults. Thus, a method that enables in vivo visualization and tissue characterization for gliosis would be invaluable for studies of CNS insults and corresponding treatments. Manganese has proven to be a useful MRI contrast agent that enters cells via Ca2+ channels and has been applied to manganese-enhanced MRI (MEMRI) for neuronal functional mapping. This study investigated whether MEMRI can detect astrogliosis after focal ischemia in vivo. Rats were divided into groups according to the number of days after either transient middle cerebral artery occlusion or a sham. Ring- or crescent-shaped enhancement of MEMRI corresponded to the GFAP-positive astroglia observed in the peripheral region of the ischemic core 11 days after middle cerebral artery occlusion. This indicates that MEMRI enhancement predominantly reflects reactive astrogliosis after stroke.

Original languageEnglish
Pages (from-to)3122-3131
Number of pages10
JournalNeuroImage
Volume49
Issue number4
DOIs
Publication statusPublished - 15-02-2010
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Neurology
  • Cognitive Neuroscience

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