TY - JOUR
T1 - Inactivation of the p53 Gene Is not Required for Tumorigenesis of Medullary Thyroid Carcinoma or Pheochromocytoma
AU - Yana, Ikuo
AU - Nakamura, Tsutomu
AU - Shin, Eisei
AU - Karakawa, Katsu
AU - Kurahashi, Hiroki
AU - Kurita, Yoshihiro
AU - Kobayashi, Tetsuro
AU - Mori, Takesada
AU - Nishisho, Isamu
AU - Takai, Shin‐ichiro ‐i
PY - 1992/11
Y1 - 1992/11
N2 - A polymerase chain reaction (PCR)‐mediated RNase protection analysis was performed to detect subtle genetic alterations of p53 in medullary thyroid carcinoma (MTC) and pheochromocytoma. None of the 30 pheochromocytomas showed abnormal RNase protection patterns. Only one of 32 MTCs showed an abnormal pattern, and subsequent DNA sequencing of the PCR product revealed that it had a G to C transversion in codon 49 that resulted in a change from aspartic acid to histidine. However, this was a sporadic MTC with no specific clinicopathological characteristics. On the basis of a previous report that genes on chromosome 17p were not deleted in MTCs and were relatively infrequently deleted in pheochromocytomas, our results suggest that the p53 gene is not involved in tumorigenesis of MTC or pheochromocytoma.
AB - A polymerase chain reaction (PCR)‐mediated RNase protection analysis was performed to detect subtle genetic alterations of p53 in medullary thyroid carcinoma (MTC) and pheochromocytoma. None of the 30 pheochromocytomas showed abnormal RNase protection patterns. Only one of 32 MTCs showed an abnormal pattern, and subsequent DNA sequencing of the PCR product revealed that it had a G to C transversion in codon 49 that resulted in a change from aspartic acid to histidine. However, this was a sporadic MTC with no specific clinicopathological characteristics. On the basis of a previous report that genes on chromosome 17p were not deleted in MTCs and were relatively infrequently deleted in pheochromocytomas, our results suggest that the p53 gene is not involved in tumorigenesis of MTC or pheochromocytoma.
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U2 - 10.1111/j.1349-7006.1992.tb02730.x
DO - 10.1111/j.1349-7006.1992.tb02730.x
M3 - Article
C2 - 1483923
AN - SCOPUS:0027066835
SN - 0910-5050
VL - 83
SP - 1113
EP - 1116
JO - Japanese Journal of Cancer Research
JF - Japanese Journal of Cancer Research
IS - 11
ER -
