Abstract
Inorganic phosphate (Pi) is required in many biological processes, including signaling cascades, skeletal development, tooth mineralization, and nucleic acid synthesis. Recently, we showed that Pi transport in osteoblasts, mediated by Slc20a1, a member of the type III sodium-dependent phosphate transporter family, is indispensable for osteoid mineralization in rapidly growing rat bone. In addition, we found that bone mineral density decreased slightly with dysfunction of Pi homeostasis in aged transgenic rats overexpressing mouse Slc20a1 (Slc20a1-Tg). Bone and tooth share certain common molecular features, and thus, we focused on tooth development in Slc20a1-Tg mandibular incisors in order to determine the role of Slc20a1 in tooth mineralization. Around the time of weaning, there were no significant differences in serologic parameters between wild-type and Slc20a1-Tg rats. However, histological analysis showed that Slc20a1-Tg ameloblasts formed clusters in the papillary layer during the maturation stage as early as 4 weeks of age. These pathologies became more severe with age and included the formation of cyst-like or multilayer ameloblast structures, accompanied by a chalky white appearance with abnormal attrition and fracture. Hyperphosphatemia was also observed in aging Slc20a1-Tg rats. Micro-computed tomography and electron probe microanalysis revealed impairments in enamel, such as delayed mineralization and hypomineralization. Our results suggest that enamel formation is sensitive to imbalances in Pit1-mediated cellular function as seen in bone, although these processes are under the control of systemic Pi homeostasis.
| Original language | English |
|---|---|
| Pages (from-to) | 192-202 |
| Number of pages | 11 |
| Journal | Calcified Tissue International |
| Volume | 89 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 01-09-2011 |
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All Science Journal Classification (ASJC) codes
- Endocrinology, Diabetes and Metabolism
- Orthopedics and Sports Medicine
- Endocrinology
Cite this
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Incisor enamel formation is impaired in transgenic rats overexpressing the type III NaPi transporter Slc20a1. / Yoshioka, Hirotaka; Yoshiko, Yuji; Minamizaki, Tomoko; Suzuki, Sayaka; Koma, Yoshiro; Nobukiyo, Asako; Sotomaru, Yusuke; Suzuki, Atsushi; Itoh, Mitsuyasu; Maeda, Norihiko.
In: Calcified Tissue International, Vol. 89, No. 3, 01.09.2011, p. 192-202.Research output: Contribution to journal › Article
TY - JOUR
T1 - Incisor enamel formation is impaired in transgenic rats overexpressing the type III NaPi transporter Slc20a1
AU - Yoshioka, Hirotaka
AU - Yoshiko, Yuji
AU - Minamizaki, Tomoko
AU - Suzuki, Sayaka
AU - Koma, Yoshiro
AU - Nobukiyo, Asako
AU - Sotomaru, Yusuke
AU - Suzuki, Atsushi
AU - Itoh, Mitsuyasu
AU - Maeda, Norihiko
PY - 2011/9/1
Y1 - 2011/9/1
N2 - Inorganic phosphate (Pi) is required in many biological processes, including signaling cascades, skeletal development, tooth mineralization, and nucleic acid synthesis. Recently, we showed that Pi transport in osteoblasts, mediated by Slc20a1, a member of the type III sodium-dependent phosphate transporter family, is indispensable for osteoid mineralization in rapidly growing rat bone. In addition, we found that bone mineral density decreased slightly with dysfunction of Pi homeostasis in aged transgenic rats overexpressing mouse Slc20a1 (Slc20a1-Tg). Bone and tooth share certain common molecular features, and thus, we focused on tooth development in Slc20a1-Tg mandibular incisors in order to determine the role of Slc20a1 in tooth mineralization. Around the time of weaning, there were no significant differences in serologic parameters between wild-type and Slc20a1-Tg rats. However, histological analysis showed that Slc20a1-Tg ameloblasts formed clusters in the papillary layer during the maturation stage as early as 4 weeks of age. These pathologies became more severe with age and included the formation of cyst-like or multilayer ameloblast structures, accompanied by a chalky white appearance with abnormal attrition and fracture. Hyperphosphatemia was also observed in aging Slc20a1-Tg rats. Micro-computed tomography and electron probe microanalysis revealed impairments in enamel, such as delayed mineralization and hypomineralization. Our results suggest that enamel formation is sensitive to imbalances in Pit1-mediated cellular function as seen in bone, although these processes are under the control of systemic Pi homeostasis.
AB - Inorganic phosphate (Pi) is required in many biological processes, including signaling cascades, skeletal development, tooth mineralization, and nucleic acid synthesis. Recently, we showed that Pi transport in osteoblasts, mediated by Slc20a1, a member of the type III sodium-dependent phosphate transporter family, is indispensable for osteoid mineralization in rapidly growing rat bone. In addition, we found that bone mineral density decreased slightly with dysfunction of Pi homeostasis in aged transgenic rats overexpressing mouse Slc20a1 (Slc20a1-Tg). Bone and tooth share certain common molecular features, and thus, we focused on tooth development in Slc20a1-Tg mandibular incisors in order to determine the role of Slc20a1 in tooth mineralization. Around the time of weaning, there were no significant differences in serologic parameters between wild-type and Slc20a1-Tg rats. However, histological analysis showed that Slc20a1-Tg ameloblasts formed clusters in the papillary layer during the maturation stage as early as 4 weeks of age. These pathologies became more severe with age and included the formation of cyst-like or multilayer ameloblast structures, accompanied by a chalky white appearance with abnormal attrition and fracture. Hyperphosphatemia was also observed in aging Slc20a1-Tg rats. Micro-computed tomography and electron probe microanalysis revealed impairments in enamel, such as delayed mineralization and hypomineralization. Our results suggest that enamel formation is sensitive to imbalances in Pit1-mediated cellular function as seen in bone, although these processes are under the control of systemic Pi homeostasis.
UR - http://www.scopus.com/inward/record.url?scp=80052738213&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80052738213&partnerID=8YFLogxK
U2 - 10.1007/s00223-011-9506-0
DO - 10.1007/s00223-011-9506-0
M3 - Article
C2 - 21643723
AN - SCOPUS:80052738213
VL - 89
SP - 192
EP - 202
JO - Calcified Tissue International
JF - Calcified Tissue International
SN - 0171-967X
IS - 3
ER -