Increase in pulmonary vascular permeability caused by increased adhesiveness of polymorphonuclear leukocytes and superoxide

T. Tanita, C. Song, S. Ueda, Yasushi Hoshikawa, Y. Ashino, M. Noda, T. Tabata, S. Suzuki, S. Ono, S. Fujimura

Research output: Contribution to journalArticle

Abstract

We report that mechanical stimulation of human neutrophils results in their accumulation in isolated rat lungs and in an increase in pulmonary vascular permeability. To determine whether reactive oxygen species were involved in this increase and, if so, whether it is mediate by xanthine oxidase metabolites, we assessed the effect of stimulated and unstimulated neutrophils, and of a superoxide scavenger, superoxide dismutase (SOD), and a xanthine oxidase inhibitor, allopurinol (ALLO) on pulmonary vascular permeability in isolated perfused lungs from Sprague-Dawley rats. Pulmonary vascular permeability in isolated rat lungs was assessed using a filtration coefficient determined by gravimetry. To quantify neutrophil accumulation in the lung, we measured myeloperoxydase (MPO). Neutrophils were stimulated by gentle agitation in a glass container for 10 s and Mac-1 was subsequently upregulated on the surface of the neutrophils. In lungs that received stimulated neutrophils, the pulmonary vascular filtration coefficient was about 5 times higher than in lungs that received unstimulated neutrophils. An increase in filtration coefficient was almost completely blocked by pretreatment with SOD or ALLO. However, the accumulation of stimulated neutrophils was not, or only partly, blocked by SOD or ALLO, respectively. We conclude that the increase in pulmonary vascular permeability caused by mechanically stimulated neutrophils was partly mediated by reactive oxygen species generated via the xanthine oxidase system.

Original languageEnglish
Pages (from-to)144-149
Number of pages6
JournalNihon Kokyūki Gakkai zasshi = the journal of the Japanese Respiratory Society
Volume36
Issue number2
Publication statusPublished - 01-01-1998

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Adhesiveness
Capillary Permeability
Superoxides
Neutrophils
Lung
Allopurinol
Xanthine Oxidase
Superoxide Dismutase
Reactive Oxygen Species
Glass
Blood Vessels
Sprague Dawley Rats

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

Tanita, T. ; Song, C. ; Ueda, S. ; Hoshikawa, Yasushi ; Ashino, Y. ; Noda, M. ; Tabata, T. ; Suzuki, S. ; Ono, S. ; Fujimura, S. / Increase in pulmonary vascular permeability caused by increased adhesiveness of polymorphonuclear leukocytes and superoxide. In: Nihon Kokyūki Gakkai zasshi = the journal of the Japanese Respiratory Society. 1998 ; Vol. 36, No. 2. pp. 144-149.
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Increase in pulmonary vascular permeability caused by increased adhesiveness of polymorphonuclear leukocytes and superoxide. / Tanita, T.; Song, C.; Ueda, S.; Hoshikawa, Yasushi; Ashino, Y.; Noda, M.; Tabata, T.; Suzuki, S.; Ono, S.; Fujimura, S.

In: Nihon Kokyūki Gakkai zasshi = the journal of the Japanese Respiratory Society, Vol. 36, No. 2, 01.01.1998, p. 144-149.

Research output: Contribution to journalArticle

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T1 - Increase in pulmonary vascular permeability caused by increased adhesiveness of polymorphonuclear leukocytes and superoxide

AU - Tanita, T.

AU - Song, C.

AU - Ueda, S.

AU - Hoshikawa, Yasushi

AU - Ashino, Y.

AU - Noda, M.

AU - Tabata, T.

AU - Suzuki, S.

AU - Ono, S.

AU - Fujimura, S.

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N2 - We report that mechanical stimulation of human neutrophils results in their accumulation in isolated rat lungs and in an increase in pulmonary vascular permeability. To determine whether reactive oxygen species were involved in this increase and, if so, whether it is mediate by xanthine oxidase metabolites, we assessed the effect of stimulated and unstimulated neutrophils, and of a superoxide scavenger, superoxide dismutase (SOD), and a xanthine oxidase inhibitor, allopurinol (ALLO) on pulmonary vascular permeability in isolated perfused lungs from Sprague-Dawley rats. Pulmonary vascular permeability in isolated rat lungs was assessed using a filtration coefficient determined by gravimetry. To quantify neutrophil accumulation in the lung, we measured myeloperoxydase (MPO). Neutrophils were stimulated by gentle agitation in a glass container for 10 s and Mac-1 was subsequently upregulated on the surface of the neutrophils. In lungs that received stimulated neutrophils, the pulmonary vascular filtration coefficient was about 5 times higher than in lungs that received unstimulated neutrophils. An increase in filtration coefficient was almost completely blocked by pretreatment with SOD or ALLO. However, the accumulation of stimulated neutrophils was not, or only partly, blocked by SOD or ALLO, respectively. We conclude that the increase in pulmonary vascular permeability caused by mechanically stimulated neutrophils was partly mediated by reactive oxygen species generated via the xanthine oxidase system.

AB - We report that mechanical stimulation of human neutrophils results in their accumulation in isolated rat lungs and in an increase in pulmonary vascular permeability. To determine whether reactive oxygen species were involved in this increase and, if so, whether it is mediate by xanthine oxidase metabolites, we assessed the effect of stimulated and unstimulated neutrophils, and of a superoxide scavenger, superoxide dismutase (SOD), and a xanthine oxidase inhibitor, allopurinol (ALLO) on pulmonary vascular permeability in isolated perfused lungs from Sprague-Dawley rats. Pulmonary vascular permeability in isolated rat lungs was assessed using a filtration coefficient determined by gravimetry. To quantify neutrophil accumulation in the lung, we measured myeloperoxydase (MPO). Neutrophils were stimulated by gentle agitation in a glass container for 10 s and Mac-1 was subsequently upregulated on the surface of the neutrophils. In lungs that received stimulated neutrophils, the pulmonary vascular filtration coefficient was about 5 times higher than in lungs that received unstimulated neutrophils. An increase in filtration coefficient was almost completely blocked by pretreatment with SOD or ALLO. However, the accumulation of stimulated neutrophils was not, or only partly, blocked by SOD or ALLO, respectively. We conclude that the increase in pulmonary vascular permeability caused by mechanically stimulated neutrophils was partly mediated by reactive oxygen species generated via the xanthine oxidase system.

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