Increase in pulmonary vascular permeability caused by increased expression of Mac-1 on the surface of polymorphonuclear leukocytes

T. Tanita, C. Song, S. Ueda, Yasushi Hoshikawa, S. Maeda, M. Noda, T. Tabata, S. Suzuki, S. Ono, S. Fujimura

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We studied the expression of adhesion molecules on the surface of human polymorphonuclear leukocytes (PMNs). The effects of mechanical stimulation were measured with a flow cytometer and pulmonary vascular injury due to accumulation of PMNs in the lungs was assessed by a gravimetric method. The accumulation of PMNs in the lungs was studied by measuring the amount of myeloperoxidase. PMNs were stimulated by gentle agitation in a glass container for 10s, Mac-1 (CD11b/CD18) was unregulated on the surface of PMNs that were mechanically stimulated. When unstimulated PMNs were exposed to isolated rat lungs, the filtration coefficient did not change from that under baseline conditions. However, when mechanically stimulated PMNs were exposed to isolated rat lungs, the filtration coefficient was about 5 times higher than that measured at baseline. When mechanically stimulated PMNs treated with anti-CD18 antibody were used, the increase in the filtration coefficient was completely blocked. The assay of myeloperoxidase revealed that PMNs stuck to isolated rat lungs only after stimulated PMNs were added. We conclude that when the adhesiveness of PMNs is increased by mechanical stimulation, these cells adhere to pulmonary vessels and increase pulmonary vascular permeability.

Original languageEnglish
Pages (from-to)396-401
Number of pages6
JournalJapanese Journal of Thoracic Diseases
Volume35
Issue number4
Publication statusPublished - 12-08-1997

Fingerprint

Capillary Permeability
Neutrophils
Lung
Peroxidase
Adhesiveness
Vascular System Injuries
Lung Injury
Glass
Anti-Idiotypic Antibodies

All Science Journal Classification (ASJC) codes

  • Pulmonary and Respiratory Medicine

Cite this

Tanita, T. ; Song, C. ; Ueda, S. ; Hoshikawa, Yasushi ; Maeda, S. ; Noda, M. ; Tabata, T. ; Suzuki, S. ; Ono, S. ; Fujimura, S. / Increase in pulmonary vascular permeability caused by increased expression of Mac-1 on the surface of polymorphonuclear leukocytes. In: Japanese Journal of Thoracic Diseases. 1997 ; Vol. 35, No. 4. pp. 396-401.
@article{8fc01ea225344fb0ac37d41e87e6f4ca,
title = "Increase in pulmonary vascular permeability caused by increased expression of Mac-1 on the surface of polymorphonuclear leukocytes",
abstract = "We studied the expression of adhesion molecules on the surface of human polymorphonuclear leukocytes (PMNs). The effects of mechanical stimulation were measured with a flow cytometer and pulmonary vascular injury due to accumulation of PMNs in the lungs was assessed by a gravimetric method. The accumulation of PMNs in the lungs was studied by measuring the amount of myeloperoxidase. PMNs were stimulated by gentle agitation in a glass container for 10s, Mac-1 (CD11b/CD18) was unregulated on the surface of PMNs that were mechanically stimulated. When unstimulated PMNs were exposed to isolated rat lungs, the filtration coefficient did not change from that under baseline conditions. However, when mechanically stimulated PMNs were exposed to isolated rat lungs, the filtration coefficient was about 5 times higher than that measured at baseline. When mechanically stimulated PMNs treated with anti-CD18 antibody were used, the increase in the filtration coefficient was completely blocked. The assay of myeloperoxidase revealed that PMNs stuck to isolated rat lungs only after stimulated PMNs were added. We conclude that when the adhesiveness of PMNs is increased by mechanical stimulation, these cells adhere to pulmonary vessels and increase pulmonary vascular permeability.",
author = "T. Tanita and C. Song and S. Ueda and Yasushi Hoshikawa and S. Maeda and M. Noda and T. Tabata and S. Suzuki and S. Ono and S. Fujimura",
year = "1997",
month = "8",
day = "12",
language = "English",
volume = "35",
pages = "396--401",
journal = "Respiratory Investigation",
issn = "2212-5345",
publisher = "Elsevier BV",
number = "4",

}

Tanita, T, Song, C, Ueda, S, Hoshikawa, Y, Maeda, S, Noda, M, Tabata, T, Suzuki, S, Ono, S & Fujimura, S 1997, 'Increase in pulmonary vascular permeability caused by increased expression of Mac-1 on the surface of polymorphonuclear leukocytes', Japanese Journal of Thoracic Diseases, vol. 35, no. 4, pp. 396-401.

Increase in pulmonary vascular permeability caused by increased expression of Mac-1 on the surface of polymorphonuclear leukocytes. / Tanita, T.; Song, C.; Ueda, S.; Hoshikawa, Yasushi; Maeda, S.; Noda, M.; Tabata, T.; Suzuki, S.; Ono, S.; Fujimura, S.

In: Japanese Journal of Thoracic Diseases, Vol. 35, No. 4, 12.08.1997, p. 396-401.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Increase in pulmonary vascular permeability caused by increased expression of Mac-1 on the surface of polymorphonuclear leukocytes

AU - Tanita, T.

AU - Song, C.

AU - Ueda, S.

AU - Hoshikawa, Yasushi

AU - Maeda, S.

AU - Noda, M.

AU - Tabata, T.

AU - Suzuki, S.

AU - Ono, S.

AU - Fujimura, S.

PY - 1997/8/12

Y1 - 1997/8/12

N2 - We studied the expression of adhesion molecules on the surface of human polymorphonuclear leukocytes (PMNs). The effects of mechanical stimulation were measured with a flow cytometer and pulmonary vascular injury due to accumulation of PMNs in the lungs was assessed by a gravimetric method. The accumulation of PMNs in the lungs was studied by measuring the amount of myeloperoxidase. PMNs were stimulated by gentle agitation in a glass container for 10s, Mac-1 (CD11b/CD18) was unregulated on the surface of PMNs that were mechanically stimulated. When unstimulated PMNs were exposed to isolated rat lungs, the filtration coefficient did not change from that under baseline conditions. However, when mechanically stimulated PMNs were exposed to isolated rat lungs, the filtration coefficient was about 5 times higher than that measured at baseline. When mechanically stimulated PMNs treated with anti-CD18 antibody were used, the increase in the filtration coefficient was completely blocked. The assay of myeloperoxidase revealed that PMNs stuck to isolated rat lungs only after stimulated PMNs were added. We conclude that when the adhesiveness of PMNs is increased by mechanical stimulation, these cells adhere to pulmonary vessels and increase pulmonary vascular permeability.

AB - We studied the expression of adhesion molecules on the surface of human polymorphonuclear leukocytes (PMNs). The effects of mechanical stimulation were measured with a flow cytometer and pulmonary vascular injury due to accumulation of PMNs in the lungs was assessed by a gravimetric method. The accumulation of PMNs in the lungs was studied by measuring the amount of myeloperoxidase. PMNs were stimulated by gentle agitation in a glass container for 10s, Mac-1 (CD11b/CD18) was unregulated on the surface of PMNs that were mechanically stimulated. When unstimulated PMNs were exposed to isolated rat lungs, the filtration coefficient did not change from that under baseline conditions. However, when mechanically stimulated PMNs were exposed to isolated rat lungs, the filtration coefficient was about 5 times higher than that measured at baseline. When mechanically stimulated PMNs treated with anti-CD18 antibody were used, the increase in the filtration coefficient was completely blocked. The assay of myeloperoxidase revealed that PMNs stuck to isolated rat lungs only after stimulated PMNs were added. We conclude that when the adhesiveness of PMNs is increased by mechanical stimulation, these cells adhere to pulmonary vessels and increase pulmonary vascular permeability.

UR - http://www.scopus.com/inward/record.url?scp=0030838095&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030838095&partnerID=8YFLogxK

M3 - Article

C2 - 9212663

AN - SCOPUS:0030838095

VL - 35

SP - 396

EP - 401

JO - Respiratory Investigation

JF - Respiratory Investigation

SN - 2212-5345

IS - 4

ER -