TY - JOUR
T1 - Increase in the Capability of Interleukin 2 Gene-Transduced Renal Cell Carcinoma Cells to Induce Cytotoxic Lymphocytes
AU - Itoh, Kyogo
AU - Umezu, Yasuiki
AU - Morita, Tatsuo
AU - Seito, Dai
AU - Augustus, Lazel B.
AU - Saya, Hideyuki
AU - Itoh, Kyogo
AU - Nakao, Masanobu
AU - Sakata, Motoko
AU - Miyajima, Jiro
AU - Masuoka, Kazuhiro
AU - Matsui, Hiroshi
N1 - Copyright:
Copyright 2016 Elsevier B.V., All rights reserved.
PY - 1994
Y1 - 1994
N2 - The immunogenicity of human cancer cells transfected with interleukin 2 (IL-2) gene, a potent vaccine candidate, has not yet been fully investigated. Human renal cell carcinoma (RCC) cells transduced with human IL-2 gene (RCC-IL-2) were investigated in vitro for the capability to induce lymphokine-activated killer (LAK) or cytotoxic T lymphocyte (CTL) activity in peripheral blood mononuclear cells (PBMC) or tumor-infiltrating lymphocyte (TIL). The RCC-IL-2 cells stimulated PBMC to demonstrate LAK activity, and also stimulated autologous TILs to proliferate and exhibit cytotoxicity relatively restricted to autologous tumor cells. In contrast, both parental RCC and RCC transduced with neomycin gene alone failed to induce these activities. These results indicate that RCC-IL-2 cells are more potent than the other RCC cells with regard to inducing cytotoxic lymphocytes against autologous tumor cells.
AB - The immunogenicity of human cancer cells transfected with interleukin 2 (IL-2) gene, a potent vaccine candidate, has not yet been fully investigated. Human renal cell carcinoma (RCC) cells transduced with human IL-2 gene (RCC-IL-2) were investigated in vitro for the capability to induce lymphokine-activated killer (LAK) or cytotoxic T lymphocyte (CTL) activity in peripheral blood mononuclear cells (PBMC) or tumor-infiltrating lymphocyte (TIL). The RCC-IL-2 cells stimulated PBMC to demonstrate LAK activity, and also stimulated autologous TILs to proliferate and exhibit cytotoxicity relatively restricted to autologous tumor cells. In contrast, both parental RCC and RCC transduced with neomycin gene alone failed to induce these activities. These results indicate that RCC-IL-2 cells are more potent than the other RCC cells with regard to inducing cytotoxic lymphocytes against autologous tumor cells.
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U2 - 10.2739/kurumemedj.41.53
DO - 10.2739/kurumemedj.41.53
M3 - Article
C2 - 7967440
AN - SCOPUS:0028190853
VL - 41
SP - 53
EP - 63
JO - Kurume Medical Journal
JF - Kurume Medical Journal
SN - 0023-5679
IS - 2
ER -