Increase in the Capability of Interleukin 2 Gene-Transduced Renal Cell Carcinoma Cells to Induce Cytotoxic Lymphocytes

Kyogo Itoh; Yasuiki Umezu; Tatsuo Morita; Dai Seito; Lazel B. Augustus; Hideyuki Saya; Kyogo Itoh; Masanobu Nakao; Motoko Sakata; Jiro Miyajima; Kazuhiro Masuoka; Hiroshi Matsui

doi:10.2739/kurumemedj.41.53

Increase in the Capability of Interleukin 2 Gene-Transduced Renal Cell Carcinoma Cells to Induce Cytotoxic Lymphocytes

Kyogo Itoh, Yasuiki Umezu, Tatsuo Morita, Dai Seito, Lazel B. Augustus, Hideyuki Saya, Kyogo Itoh, Masanobu Nakao, Motoko Sakata, Jiro Miyajima, Kazuhiro Masuoka, Hiroshi Matsui

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

The immunogenicity of human cancer cells transfected with interleukin 2 (IL-2) gene, a potent vaccine candidate, has not yet been fully investigated. Human renal cell carcinoma (RCC) cells transduced with human IL-2 gene (RCC-IL-2) were investigated in vitro for the capability to induce lymphokine-activated killer (LAK) or cytotoxic T lymphocyte (CTL) activity in peripheral blood mononuclear cells (PBMC) or tumor-infiltrating lymphocyte (TIL). The RCC-IL-2 cells stimulated PBMC to demonstrate LAK activity, and also stimulated autologous TILs to proliferate and exhibit cytotoxicity relatively restricted to autologous tumor cells. In contrast, both parental RCC and RCC transduced with neomycin gene alone failed to induce these activities. These results indicate that RCC-IL-2 cells are more potent than the other RCC cells with regard to inducing cytotoxic lymphocytes against autologous tumor cells.

Original language	English
Pages (from-to)	53-63
Number of pages	11
Journal	The Kurume Medical Journal
Volume	41
Issue number	2
DOIs	https://doi.org/10.2739/kurumemedj.41.53
Publication status	Published - 1994
Externally published	Yes

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General Medicine

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.2739/kurumemedj.41.53

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title = "Increase in the Capability of Interleukin 2 Gene-Transduced Renal Cell Carcinoma Cells to Induce Cytotoxic Lymphocytes",

abstract = "The immunogenicity of human cancer cells transfected with interleukin 2 (IL-2) gene, a potent vaccine candidate, has not yet been fully investigated. Human renal cell carcinoma (RCC) cells transduced with human IL-2 gene (RCC-IL-2) were investigated in vitro for the capability to induce lymphokine-activated killer (LAK) or cytotoxic T lymphocyte (CTL) activity in peripheral blood mononuclear cells (PBMC) or tumor-infiltrating lymphocyte (TIL). The RCC-IL-2 cells stimulated PBMC to demonstrate LAK activity, and also stimulated autologous TILs to proliferate and exhibit cytotoxicity relatively restricted to autologous tumor cells. In contrast, both parental RCC and RCC transduced with neomycin gene alone failed to induce these activities. These results indicate that RCC-IL-2 cells are more potent than the other RCC cells with regard to inducing cytotoxic lymphocytes against autologous tumor cells.",

keywords = "cytotoxic T lymphocytes, gene transduction, immunogenicity, interleukin 2 gene, renal cell carcinoma",

author = "Kyogo Itoh and Yasuiki Umezu and Tatsuo Morita and Dai Seito and Augustus, {Lazel B.} and Hideyuki Saya and Kyogo Itoh and Masanobu Nakao and Motoko Sakata and Jiro Miyajima and Kazuhiro Masuoka and Hiroshi Matsui",

year = "1994",

doi = "10.2739/kurumemedj.41.53",

language = "English",

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pages = "53--63",

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publisher = "Kurume University School of Medicine",

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T1 - Increase in the Capability of Interleukin 2 Gene-Transduced Renal Cell Carcinoma Cells to Induce Cytotoxic Lymphocytes

AU - Itoh, Kyogo

AU - Umezu, Yasuiki

AU - Morita, Tatsuo

AU - Seito, Dai

AU - Augustus, Lazel B.

AU - Saya, Hideyuki

AU - Itoh, Kyogo

AU - Nakao, Masanobu

AU - Sakata, Motoko

AU - Miyajima, Jiro

AU - Masuoka, Kazuhiro

AU - Matsui, Hiroshi

PY - 1994

Y1 - 1994

N2 - The immunogenicity of human cancer cells transfected with interleukin 2 (IL-2) gene, a potent vaccine candidate, has not yet been fully investigated. Human renal cell carcinoma (RCC) cells transduced with human IL-2 gene (RCC-IL-2) were investigated in vitro for the capability to induce lymphokine-activated killer (LAK) or cytotoxic T lymphocyte (CTL) activity in peripheral blood mononuclear cells (PBMC) or tumor-infiltrating lymphocyte (TIL). The RCC-IL-2 cells stimulated PBMC to demonstrate LAK activity, and also stimulated autologous TILs to proliferate and exhibit cytotoxicity relatively restricted to autologous tumor cells. In contrast, both parental RCC and RCC transduced with neomycin gene alone failed to induce these activities. These results indicate that RCC-IL-2 cells are more potent than the other RCC cells with regard to inducing cytotoxic lymphocytes against autologous tumor cells.

AB - The immunogenicity of human cancer cells transfected with interleukin 2 (IL-2) gene, a potent vaccine candidate, has not yet been fully investigated. Human renal cell carcinoma (RCC) cells transduced with human IL-2 gene (RCC-IL-2) were investigated in vitro for the capability to induce lymphokine-activated killer (LAK) or cytotoxic T lymphocyte (CTL) activity in peripheral blood mononuclear cells (PBMC) or tumor-infiltrating lymphocyte (TIL). The RCC-IL-2 cells stimulated PBMC to demonstrate LAK activity, and also stimulated autologous TILs to proliferate and exhibit cytotoxicity relatively restricted to autologous tumor cells. In contrast, both parental RCC and RCC transduced with neomycin gene alone failed to induce these activities. These results indicate that RCC-IL-2 cells are more potent than the other RCC cells with regard to inducing cytotoxic lymphocytes against autologous tumor cells.

KW - cytotoxic T lymphocytes

KW - gene transduction

KW - immunogenicity

KW - interleukin 2 gene

KW - renal cell carcinoma

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DO - 10.2739/kurumemedj.41.53

M3 - Article

C2 - 7967440

AN - SCOPUS:0028190853

SN - 0023-5679

VL - 41

SP - 53

EP - 63

JO - The Kurume Medical Journal

JF - The Kurume Medical Journal

IS - 2

ER -

Increase in the Capability of Interleukin 2 Gene-Transduced Renal Cell Carcinoma Cells to Induce Cytotoxic Lymphocytes

Abstract

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