Increase of transcriptional levels of egr-1 and nur77 genes due to both nicotine treatment and withdrawal in pheochromocytoma cells

Naohiro Ichino, Koji Yamada, K. Nishii, H. Sawada, T. Nagatsu, H. Ishiguro

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The influence of nicotine on the expression of egr-1 and nur77 genes by nicotine treatment and withdrawal was assessed using PC12 cells. Nicotine treatment significantly increased the amount of mRNA for egr-1 and nur77 genes at 0.5h post-nicotine treatment in the PC12 cells. In addition, nicotine withdrawal also elevated transcriptional levels of egr-1 and nur77 genes in Northern blot analyses. Nicotine treatment (200 μM) was also found to significantly increase expressional levels of Egr-1 and Nur77 proteins at 0.5h post-nicotine treatment. In contrast, Egr-1 and Nur77 protein levels were dramatically decreased by nicotine withdrawal. These results suggest that expressional levels of Egr-1 and Nur77 proteins in neural cells may affect the transcriptional activity of late-response genes after nicotine withdrawal.

Original languageEnglish
Pages (from-to)1015-1022
Number of pages8
JournalJournal of Neural Transmission
Volume109
Issue number7-8
DOIs
Publication statusPublished - 23-07-2002

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Pheochromocytoma
Nicotine
Genes
PC12 Cells
Proteins
Northern Blotting
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Neurology
  • Clinical Neurology
  • Psychiatry and Mental health
  • Biological Psychiatry

Cite this

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abstract = "The influence of nicotine on the expression of egr-1 and nur77 genes by nicotine treatment and withdrawal was assessed using PC12 cells. Nicotine treatment significantly increased the amount of mRNA for egr-1 and nur77 genes at 0.5h post-nicotine treatment in the PC12 cells. In addition, nicotine withdrawal also elevated transcriptional levels of egr-1 and nur77 genes in Northern blot analyses. Nicotine treatment (200 μM) was also found to significantly increase expressional levels of Egr-1 and Nur77 proteins at 0.5h post-nicotine treatment. In contrast, Egr-1 and Nur77 protein levels were dramatically decreased by nicotine withdrawal. These results suggest that expressional levels of Egr-1 and Nur77 proteins in neural cells may affect the transcriptional activity of late-response genes after nicotine withdrawal.",
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Increase of transcriptional levels of egr-1 and nur77 genes due to both nicotine treatment and withdrawal in pheochromocytoma cells. / Ichino, Naohiro; Yamada, Koji; Nishii, K.; Sawada, H.; Nagatsu, T.; Ishiguro, H.

In: Journal of Neural Transmission, Vol. 109, No. 7-8, 23.07.2002, p. 1015-1022.

Research output: Contribution to journalArticle

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T1 - Increase of transcriptional levels of egr-1 and nur77 genes due to both nicotine treatment and withdrawal in pheochromocytoma cells

AU - Ichino, Naohiro

AU - Yamada, Koji

AU - Nishii, K.

AU - Sawada, H.

AU - Nagatsu, T.

AU - Ishiguro, H.

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