Increased expression of signal proteins in mouse brain by withdrawal of nicotine after chronic administration of the alkaloid

Kouji Yamada, Naohiro Ichino, Kazuhiro Nishii, Hirohide Sawada, Takehiko Hida, Hiroshi Ishiguro

Research output: Contribution to journalArticle

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Abstract

We previously reported that nicotine withdrawal up-regulates transcription of some immediately early genes (IEGs), signal transduction-related genes, and apoptosis-related genes in cultures of pheochromocytoma PC12 cells, which are of neuronal lineage (Ichino et al., 1999; Ichino et al., 2002; Yamada et al., 2005). In the present study, we aimed at further elucidating the effects of nicotine withdrawal on the expression of the downstream proteins of IEGs in the brain from mice after the chronic administration of the alkaloid to them. We observed significant increases in the protein levels of Bag-1, Rab2, Hsp70, and Raf1 by Western blot analysis. The present results suggest that such changes may also occur in the brain of smokers during abstaining from smoking and may be related to their nicotine withdrawal symptoms.

Original languageEnglish
Pages (from-to)112-120
Number of pages9
JournalBiogenic Amines
Volume20
Issue number3-4
Publication statusPublished - 01-12-2006

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Nicotine
Alkaloids
Brain
Genes
Proteins
Substance Withdrawal Syndrome
PC12 Cells
Pheochromocytoma
Signal Transduction
Up-Regulation
Western Blotting
Smoking
Apoptosis

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Pharmacology

Cite this

Yamada, Kouji ; Ichino, Naohiro ; Nishii, Kazuhiro ; Sawada, Hirohide ; Hida, Takehiko ; Ishiguro, Hiroshi. / Increased expression of signal proteins in mouse brain by withdrawal of nicotine after chronic administration of the alkaloid. In: Biogenic Amines. 2006 ; Vol. 20, No. 3-4. pp. 112-120.
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Increased expression of signal proteins in mouse brain by withdrawal of nicotine after chronic administration of the alkaloid. / Yamada, Kouji; Ichino, Naohiro; Nishii, Kazuhiro; Sawada, Hirohide; Hida, Takehiko; Ishiguro, Hiroshi.

In: Biogenic Amines, Vol. 20, No. 3-4, 01.12.2006, p. 112-120.

Research output: Contribution to journalArticle

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