Increased levels of pregnancy-associated plasma protein-A2 in the serum of pre-eclamptic patients

Haruki Nishizawa, Kanako Pryor-Koishi, Machiko Suzuki, Takema Kato, Hiroshi Kogo, Takao Sekiya, Hiroki Kurahashi, Yasuhiro Udagawa

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Pregnancy-associated plasma protein-A and -A2 (PAPP-A and -A2) are proteases that cleave insulin-like growth factor-binding proteins (IGFBPs), resulting in local activation of IGF signaling pathways. Here, we examined PAPP-A and -A2 mRNA and protein levels in placenta and maternal sera from women with pre-eclampsia and compared them with samples from uncomplicated pregnancy. PAPP-A2 but not PAPP-A mRNA and protein were elevated in pre-eclamptic placenta (P < 0.01). PAPP-A2 is normally produced in placental syncytiotrophoblast cells and maternal decidua. PAPP-A2 in syncytiotrophoblast cells was dramatically increased in pre-eclampsia. Maternal serum concentrations of PAPP-A2 but not PAPP-A were also significantly elevated in pre-eclampsia as compared with uncomplicated pregnancy. mRNA levels of IGFBP5, a specific substrate for PAPP-A2 protease activity, were also significantly increased, suggesting a potential role for IGFBP5 in fetal and placental growth suppression during pre-eclampsia. However, IGFBP5 protein levels were not increased in placenta from pre-eclampsia, possibly due to cleavage by up-regulated PAPP-A2. These data might imply that PAPP-A2 may be up-regulated in pre-eclamptic pregnancy to compensate for IGFBP5-mediated suppression of the IGF pathway, although final birthweights are still low in pre-eclamptic pregnancy.

Original languageEnglish
Pages (from-to)595-602
Number of pages8
JournalMolecular Human Reproduction
Volume14
Issue number10
DOIs
Publication statusPublished - 01-12-2008

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LY 165163
varespladib methyl
Blood Proteins
Pregnancy-Associated Plasma Protein-A
Pregnancy
Pre-Eclampsia
Serum
Placenta
Mothers
Trophoblasts
Messenger RNA
Peptide Hydrolases
Decidua
Insulin-Like Growth Factor Binding Proteins
Proteins
Fetal Development

All Science Journal Classification (ASJC) codes

  • Reproductive Medicine
  • Embryology
  • Molecular Biology
  • Genetics
  • Obstetrics and Gynaecology
  • Developmental Biology
  • Cell Biology

Cite this

Nishizawa, Haruki ; Pryor-Koishi, Kanako ; Suzuki, Machiko ; Kato, Takema ; Kogo, Hiroshi ; Sekiya, Takao ; Kurahashi, Hiroki ; Udagawa, Yasuhiro. / Increased levels of pregnancy-associated plasma protein-A2 in the serum of pre-eclamptic patients. In: Molecular Human Reproduction. 2008 ; Vol. 14, No. 10. pp. 595-602.
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Increased levels of pregnancy-associated plasma protein-A2 in the serum of pre-eclamptic patients. / Nishizawa, Haruki; Pryor-Koishi, Kanako; Suzuki, Machiko; Kato, Takema; Kogo, Hiroshi; Sekiya, Takao; Kurahashi, Hiroki; Udagawa, Yasuhiro.

In: Molecular Human Reproduction, Vol. 14, No. 10, 01.12.2008, p. 595-602.

Research output: Contribution to journalArticle

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