Increased regulatory B cells are involved in immune evasion in patients with gastric cancer

Yuki Murakami, Hiroaki Saito, Shota Shimizu, Yusuke Kono, Yuji Shishido, Kozo Miyatani, Tomoyuki Matsunaga, Yoji Fukumoto, Keigo Ashida, Tomohiko Sakabe, Yuji Nakayama, Yoshiyuki Fujiwara

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3 Citations (Scopus)

Abstract

Accumulating evidence has indicated that immune regulatory cells are involved in the establishment of tumoral immune evasion. However, the role of regulatory B cells (Bregs) in this remains unclear. Here, we identified a role for Bregs in immune evasion in gastric cancer (GC) patients. The frequency of peripheral Bregs was significantly higher in GC patients than in healthy controls (P = 0.0023). Moreover, the frequency of CD19+CD24hiCD27+ B cells in GC tissue was significantly higher than in peripheral blood and healthy gastric tissue. Carboxyfluorescein succinimidyl ester labeling revealed that CD19+CD24hiCD27+ B cells could suppress the proliferation of autologous CD4+ T cells. Moreover, CD19+CD24hiCD27+ B cells inhibited the production of interferon-gamma by CD4+ T cells. Double staining immunohistochemistry of interleukin-10 and CD19 revealed 5-year overall survival rates of 65.4% and 13.3% in BregLow and BregHigh groups, respectively (P < 0.0001). Multivariate analysis indicated that the frequency of Bregs was an independent prognostic indicator in GC patients. Taken together, our results show the existence of Bregs in GC tissue, and indicate that they are significantly correlated with the prognosis of GC patients.

Original languageEnglish
Article number13083
JournalScientific reports
Volume9
Issue number1
DOIs
Publication statusPublished - 01-12-2019
Externally publishedYes

All Science Journal Classification (ASJC) codes

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    Murakami, Y., Saito, H., Shimizu, S., Kono, Y., Shishido, Y., Miyatani, K., Matsunaga, T., Fukumoto, Y., Ashida, K., Sakabe, T., Nakayama, Y., & Fujiwara, Y. (2019). Increased regulatory B cells are involved in immune evasion in patients with gastric cancer. Scientific reports, 9(1), [13083]. https://doi.org/10.1038/s41598-019-49581-4