Increased Serum GP88 (Progranulin) Concentrations in Rheumatoid Arthritis

Yasuko Yamamoto, Masao Takemura, Ginette Serrero, Jun Hayashi, Binbin Yue, Aya Tsuboi, Hisako Kubo, Takashi Mitsuhashi, Kenji Mannami, Masao Sato, Hidetoshi Matsunami, Yushi Matuo, Kuniaki Saito

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

GP88 (Progranulin; PGRN) is a secreted glycosylated protein with important functions in several processes, including immune response and cancer growth. Recent reports have shown that PGRN is a therapeutic target for rheumatoid arthritis (RA) because of its capability to bind with tumor necrosis factor receptor (TNFR). However, the serum PGRN level in RA patients has not been investigated. We used enzyme-linked immunosorbent assay (ELISA) to quantify the serum levels of PGRN in 417 healthy subjects, 56 patients with RA and 31 patients with osteoarthritis (OA). In RA patients, we also measured the serum TNF-α and sTNFR concentration. Immunohistochemical staining of PGRN was performed using synovectomy tissue of RA patients. The serum PGRN normal range was established as 40.1 ± 8.7 ng/ml. PGRN levels were not influenced by sex or age. A significant increase in serum PGRN levels was observed in RA (50.2 ± 11.1 ng/ml) and OA (45.4 ± 6.6 ng/ml) groups compared to those in age-matched healthy controls (40.4 ± 9.9 ng/ml) (p < 0.05, Tukey). Further, PGRN levels in the synovial fluid of RA patients (68.4 ± 3.4 ng/ml) were found to be significantly higher than those in OA patients (35.9 ± 16.8 ng/ml). Immunohistochemical staining of PGRN revealed that the highest positive signal was detected in macrophages. Circulating PGRN in RA patients was weakly associated with TNF-α and sTNFR 2 concentration. Furthermore, PGRN/TNF-α ratio was correlated the stage of the disease in RA patients. The concentrations of serum PGRN in RA were found to be significantly higher than those in age-matched healthy controls, although it remains to be clarified how blood PGRN is related to the pathogenesis of RA. Our results showed that the serum PGRN may be a useful approach to monitor the disease activity in RA patients.

Original languageEnglish
Pages (from-to)1806-1813
Number of pages8
JournalInflammation
Volume37
Issue number5
DOIs
Publication statusPublished - 24-09-2014
Externally publishedYes

Fingerprint

Rheumatoid Arthritis
Serum
Osteoarthritis
Staining and Labeling
Tumor Necrosis Factor Receptors
Synovial Fluid
Healthy Volunteers
Reference Values
Enzyme-Linked Immunosorbent Assay
Macrophages
Growth

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Yamamoto, Yasuko ; Takemura, Masao ; Serrero, Ginette ; Hayashi, Jun ; Yue, Binbin ; Tsuboi, Aya ; Kubo, Hisako ; Mitsuhashi, Takashi ; Mannami, Kenji ; Sato, Masao ; Matsunami, Hidetoshi ; Matuo, Yushi ; Saito, Kuniaki. / Increased Serum GP88 (Progranulin) Concentrations in Rheumatoid Arthritis. In: Inflammation. 2014 ; Vol. 37, No. 5. pp. 1806-1813.
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Yamamoto, Y, Takemura, M, Serrero, G, Hayashi, J, Yue, B, Tsuboi, A, Kubo, H, Mitsuhashi, T, Mannami, K, Sato, M, Matsunami, H, Matuo, Y & Saito, K 2014, 'Increased Serum GP88 (Progranulin) Concentrations in Rheumatoid Arthritis', Inflammation, vol. 37, no. 5, pp. 1806-1813. https://doi.org/10.1007/s10753-014-9911-4

Increased Serum GP88 (Progranulin) Concentrations in Rheumatoid Arthritis. / Yamamoto, Yasuko; Takemura, Masao; Serrero, Ginette; Hayashi, Jun; Yue, Binbin; Tsuboi, Aya; Kubo, Hisako; Mitsuhashi, Takashi; Mannami, Kenji; Sato, Masao; Matsunami, Hidetoshi; Matuo, Yushi; Saito, Kuniaki.

In: Inflammation, Vol. 37, No. 5, 24.09.2014, p. 1806-1813.

Research output: Contribution to journalArticle

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T1 - Increased Serum GP88 (Progranulin) Concentrations in Rheumatoid Arthritis

AU - Yamamoto, Yasuko

AU - Takemura, Masao

AU - Serrero, Ginette

AU - Hayashi, Jun

AU - Yue, Binbin

AU - Tsuboi, Aya

AU - Kubo, Hisako

AU - Mitsuhashi, Takashi

AU - Mannami, Kenji

AU - Sato, Masao

AU - Matsunami, Hidetoshi

AU - Matuo, Yushi

AU - Saito, Kuniaki

PY - 2014/9/24

Y1 - 2014/9/24

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Yamamoto Y, Takemura M, Serrero G, Hayashi J, Yue B, Tsuboi A et al. Increased Serum GP88 (Progranulin) Concentrations in Rheumatoid Arthritis. Inflammation. 2014 Sep 24;37(5):1806-1813. https://doi.org/10.1007/s10753-014-9911-4