Increased T-cell responses to Epstein-Barr virus with high viral load in patients with Epstein-Barr virus-positive diffuse large B-cell lymphoma

Satoko Morishima, Shigeo Nakamura, Kazuhito Yamamoto, Hidemasa Miyauchi, Yoshitoyo Kagami, Tomohiro Kinoshita, Hiroshi Onoda, Yasushi Yatabe, Masafumi Ito, Kouichi Miyamura, Hirokazu Nagai, Suzuko Moritani, Isamu Sugiura, Keitaro Tsushita, Hidetsugu Mihara, Kaneyuki Ohbayashi, Sachiko Iba, Nobuhiko Emi, Masataka Okamoto, Seiko IwataHiroshi Kimura, Kiyotaka Kuzushima, Yasuo Morishima

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

Abstract The immunological status of patients with Epstein-Barr virus-positive diffuse large B-cell lymphoma (EBV+ DLBCL) without obvious immunodeficiency has not been elucidated. A multicenter prospective study was conducted to assess pretreatment T-cell responses to EBV, EBV-DNA load and anti-EBV antibody in these patients. The proliferative and interferon (IFN)-γ-producing capacity of T-cells in response to autologous B-lymphoblastoid cell lines was determined using carboxyfluorescein diacetate succinimidyl ester (CFSE)-based assay. Frequencies of EBV-specific CD4+ T-cells in patients with EBV+ DLBCL (n = 13) were significantly higher than in healthy controls (HCs) (n = 16) after both ex vivo and in vitro stimulation. Frequencies of EBV-specific CD8+ T-cells in patients with EBV+ DLBCL tended to be higher than in HCs after in vitro stimulation. Patients with EBV+ DLBCL also showed increased immunoglobulin G (IgG) responses to lytic EBV-encoded antigens. Pretreatment plasma EBV-DNA level was significantly higher in patients with EBV+ DLBCL than in patients with EBV- DLBCL or HCs. In conclusion, EBV-specific T-cells showed increased reactivity, accompanied by higher levels of plasma virus DNA in patients with EBV+ DLBCL.

Original languageEnglish
Pages (from-to)1072-1078
Number of pages7
JournalLeukemia and Lymphoma
Volume56
Issue number4
DOIs
Publication statusPublished - 01-04-2015

All Science Journal Classification (ASJC) codes

  • Hematology
  • Oncology
  • Cancer Research

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