Increased T-cell responses to Epstein-Barr virus with high viral load in patients with Epstein-Barr virus-positive diffuse large B-cell lymphoma

Satoko Morishima; Shigeo Nakamura; Kazuhito Yamamoto; Hidemasa Miyauchi; Yoshitoyo Kagami; Tomohiro Kinoshita; Hiroshi Onoda; Yasushi Yatabe; Masafumi Ito; Kouichi Miyamura; Hirokazu Nagai; Suzuko Moritani; Isamu Sugiura; Keitaro Tsushita; Hidetsugu Mihara; Kaneyuki Ohbayashi; Sachiko Iba; Nobuhiko Emi; Masataka Okamoto; Seiko Iwata; Hiroshi Kimura; Kiyotaka Kuzushima; Yasuo Morishima

doi:10.3109/10428194.2014.938326

Increased T-cell responses to Epstein-Barr virus with high viral load in patients with Epstein-Barr virus-positive diffuse large B-cell lymphoma

Satoko Morishima
, Shigeo Nakamura
, Kazuhito Yamamoto
, Hidemasa Miyauchi
, Yoshitoyo Kagami
, Tomohiro Kinoshita
, Hiroshi Onoda
, Yasushi Yatabe
, Masafumi Ito
, Kouichi Miyamura
, Hirokazu Nagai
, Suzuko Moritani
, Isamu Sugiura
, Keitaro Tsushita
, Hidetsugu Mihara
, Kaneyuki Ohbayashi
, Sachiko Iba
, Nobuhiko Emi
, Masataka Okamoto
, Seiko Iwata

Hiroshi Kimura, Kiyotaka Kuzushima, Yasuo Morishima

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Abstract The immunological status of patients with Epstein-Barr virus-positive diffuse large B-cell lymphoma (EBV+ DLBCL) without obvious immunodeficiency has not been elucidated. A multicenter prospective study was conducted to assess pretreatment T-cell responses to EBV, EBV-DNA load and anti-EBV antibody in these patients. The proliferative and interferon (IFN)-γ-producing capacity of T-cells in response to autologous B-lymphoblastoid cell lines was determined using carboxyfluorescein diacetate succinimidyl ester (CFSE)-based assay. Frequencies of EBV-specific CD4+ T-cells in patients with EBV+ DLBCL (n = 13) were significantly higher than in healthy controls (HCs) (n = 16) after both ex vivo and in vitro stimulation. Frequencies of EBV-specific CD8+ T-cells in patients with EBV+ DLBCL tended to be higher than in HCs after in vitro stimulation. Patients with EBV+ DLBCL also showed increased immunoglobulin G (IgG) responses to lytic EBV-encoded antigens. Pretreatment plasma EBV-DNA level was significantly higher in patients with EBV+ DLBCL than in patients with EBV- DLBCL or HCs. In conclusion, EBV-specific T-cells showed increased reactivity, accompanied by higher levels of plasma virus DNA in patients with EBV+ DLBCL.

Original language	English
Pages (from-to)	1072-1078
Number of pages	7
Journal	Leukemia and Lymphoma
Volume	56
Issue number	4
DOIs	https://doi.org/10.3109/10428194.2014.938326
Publication status	Published - 01-04-2015
Externally published	Yes

All Science Journal Classification (ASJC) codes

Hematology
Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3109/10428194.2014.938326

Cite this

Morishima, S., Nakamura, S., Yamamoto, K., Miyauchi, H., Kagami, Y., Kinoshita, T., Onoda, H., Yatabe, Y., Ito, M., Miyamura, K., Nagai, H., Moritani, S., Sugiura, I., Tsushita, K., Mihara, H., Ohbayashi, K., Iba, S., Emi, N., Okamoto, M., ... Morishima, Y. (2015). Increased T-cell responses to Epstein-Barr virus with high viral load in patients with Epstein-Barr virus-positive diffuse large B-cell lymphoma. Leukemia and Lymphoma, 56(4), 1072-1078. https://doi.org/10.3109/10428194.2014.938326

@article{c8551554ebc0402099a7bbb6b8d01fad,

title = "Increased T-cell responses to Epstein-Barr virus with high viral load in patients with Epstein-Barr virus-positive diffuse large B-cell lymphoma",

abstract = "Abstract The immunological status of patients with Epstein-Barr virus-positive diffuse large B-cell lymphoma (EBV+ DLBCL) without obvious immunodeficiency has not been elucidated. A multicenter prospective study was conducted to assess pretreatment T-cell responses to EBV, EBV-DNA load and anti-EBV antibody in these patients. The proliferative and interferon (IFN)-γ-producing capacity of T-cells in response to autologous B-lymphoblastoid cell lines was determined using carboxyfluorescein diacetate succinimidyl ester (CFSE)-based assay. Frequencies of EBV-specific CD4+ T-cells in patients with EBV+ DLBCL (n = 13) were significantly higher than in healthy controls (HCs) (n = 16) after both ex vivo and in vitro stimulation. Frequencies of EBV-specific CD8+ T-cells in patients with EBV+ DLBCL tended to be higher than in HCs after in vitro stimulation. Patients with EBV+ DLBCL also showed increased immunoglobulin G (IgG) responses to lytic EBV-encoded antigens. Pretreatment plasma EBV-DNA level was significantly higher in patients with EBV+ DLBCL than in patients with EBV- DLBCL or HCs. In conclusion, EBV-specific T-cells showed increased reactivity, accompanied by higher levels of plasma virus DNA in patients with EBV+ DLBCL.",

author = "Satoko Morishima and Shigeo Nakamura and Kazuhito Yamamoto and Hidemasa Miyauchi and Yoshitoyo Kagami and Tomohiro Kinoshita and Hiroshi Onoda and Yasushi Yatabe and Masafumi Ito and Kouichi Miyamura and Hirokazu Nagai and Suzuko Moritani and Isamu Sugiura and Keitaro Tsushita and Hidetsugu Mihara and Kaneyuki Ohbayashi and Sachiko Iba and Nobuhiko Emi and Masataka Okamoto and Seiko Iwata and Hiroshi Kimura and Kiyotaka Kuzushima and Yasuo Morishima",

note = "Publisher Copyright: {\textcopyright} 2014 Informa UK, Ltd.",

year = "2015",

month = apr,

day = "1",

doi = "10.3109/10428194.2014.938326",

language = "English",

volume = "56",

pages = "1072--1078",

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Morishima, S, Nakamura, S, Yamamoto, K, Miyauchi, H, Kagami, Y, Kinoshita, T, Onoda, H, Yatabe, Y, Ito, M, Miyamura, K, Nagai, H, Moritani, S, Sugiura, I, Tsushita, K, Mihara, H, Ohbayashi, K, Iba, S, Emi, N, Okamoto, M, Iwata, S, Kimura, H, Kuzushima, K & Morishima, Y 2015, 'Increased T-cell responses to Epstein-Barr virus with high viral load in patients with Epstein-Barr virus-positive diffuse large B-cell lymphoma', Leukemia and Lymphoma, vol. 56, no. 4, pp. 1072-1078. https://doi.org/10.3109/10428194.2014.938326

TY - JOUR

T1 - Increased T-cell responses to Epstein-Barr virus with high viral load in patients with Epstein-Barr virus-positive diffuse large B-cell lymphoma

AU - Morishima, Satoko

AU - Nakamura, Shigeo

AU - Yamamoto, Kazuhito

AU - Miyauchi, Hidemasa

AU - Kagami, Yoshitoyo

AU - Kinoshita, Tomohiro

AU - Onoda, Hiroshi

AU - Yatabe, Yasushi

AU - Ito, Masafumi

AU - Miyamura, Kouichi

AU - Nagai, Hirokazu

AU - Moritani, Suzuko

AU - Sugiura, Isamu

AU - Tsushita, Keitaro

AU - Mihara, Hidetsugu

AU - Ohbayashi, Kaneyuki

AU - Iba, Sachiko

AU - Emi, Nobuhiko

AU - Okamoto, Masataka

AU - Iwata, Seiko

AU - Kimura, Hiroshi

AU - Kuzushima, Kiyotaka

AU - Morishima, Yasuo

PY - 2015/4/1

Y1 - 2015/4/1

N2 - Abstract The immunological status of patients with Epstein-Barr virus-positive diffuse large B-cell lymphoma (EBV+ DLBCL) without obvious immunodeficiency has not been elucidated. A multicenter prospective study was conducted to assess pretreatment T-cell responses to EBV, EBV-DNA load and anti-EBV antibody in these patients. The proliferative and interferon (IFN)-γ-producing capacity of T-cells in response to autologous B-lymphoblastoid cell lines was determined using carboxyfluorescein diacetate succinimidyl ester (CFSE)-based assay. Frequencies of EBV-specific CD4+ T-cells in patients with EBV+ DLBCL (n = 13) were significantly higher than in healthy controls (HCs) (n = 16) after both ex vivo and in vitro stimulation. Frequencies of EBV-specific CD8+ T-cells in patients with EBV+ DLBCL tended to be higher than in HCs after in vitro stimulation. Patients with EBV+ DLBCL also showed increased immunoglobulin G (IgG) responses to lytic EBV-encoded antigens. Pretreatment plasma EBV-DNA level was significantly higher in patients with EBV+ DLBCL than in patients with EBV- DLBCL or HCs. In conclusion, EBV-specific T-cells showed increased reactivity, accompanied by higher levels of plasma virus DNA in patients with EBV+ DLBCL.

AB - Abstract The immunological status of patients with Epstein-Barr virus-positive diffuse large B-cell lymphoma (EBV+ DLBCL) without obvious immunodeficiency has not been elucidated. A multicenter prospective study was conducted to assess pretreatment T-cell responses to EBV, EBV-DNA load and anti-EBV antibody in these patients. The proliferative and interferon (IFN)-γ-producing capacity of T-cells in response to autologous B-lymphoblastoid cell lines was determined using carboxyfluorescein diacetate succinimidyl ester (CFSE)-based assay. Frequencies of EBV-specific CD4+ T-cells in patients with EBV+ DLBCL (n = 13) were significantly higher than in healthy controls (HCs) (n = 16) after both ex vivo and in vitro stimulation. Frequencies of EBV-specific CD8+ T-cells in patients with EBV+ DLBCL tended to be higher than in HCs after in vitro stimulation. Patients with EBV+ DLBCL also showed increased immunoglobulin G (IgG) responses to lytic EBV-encoded antigens. Pretreatment plasma EBV-DNA level was significantly higher in patients with EBV+ DLBCL than in patients with EBV- DLBCL or HCs. In conclusion, EBV-specific T-cells showed increased reactivity, accompanied by higher levels of plasma virus DNA in patients with EBV+ DLBCL.

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UR - https://www.scopus.com/pages/publications/84929087777#tab=citedBy

U2 - 10.3109/10428194.2014.938326

DO - 10.3109/10428194.2014.938326

M3 - Article

C2 - 24975317

AN - SCOPUS:84929087777

SN - 1042-8194

VL - 56

SP - 1072

EP - 1078

JO - Leukemia and Lymphoma

JF - Leukemia and Lymphoma

IS - 4

ER -

Increased T-cell responses to Epstein-Barr virus with high viral load in patients with Epstein-Barr virus-positive diffuse large B-cell lymphoma

Abstract

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