Increased urethral resistance by periurethral injection of low serum cultured adipose-derived mesenchymal stromal cells in rats

Tatsuhito Watanabe, Shoichi Maruyama, Tokunori Yamamoto, Izumi Kamo, Kaoru Yasuda, Yosuke Saka, Takenori Ozaki, Yukio Yuzawa, Seiichi Matsuo, Momokazu Gotoh

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Objectives: To evaluate the effects of a periurethral injection of low serum cultured adipose tissue-derived mesenchymal stromal cells (LASC) and to develop a new autologous cell therapy for stress urinary incontinence. Methods: F344 rats were divided into three groups as based on the periurethral injection of LASC, GAX collagen or vehicle (control). At 2 and 4weeks after injection, leak point pressure (LPP) was measured before and after transection of the pelvic nerves. For cell tracking, LASC of green fluorescent protein transgenic rats were injected into nude rats. Results: At 2weeks, both the LASC and collagen groups showed significantly higher LPP than the control group. At 4weeks, the increase in LPP in the LASC group remained, whereas LPP in the collagen group decreased to baseline levels. In the absence of the urethral closure reflex after transection of the pelvic nerves, LPP in the LASC group was significantly higher than that in the other two groups. Histologically, the size of the urethral lumen was smaller in the LASC group than the collagen group. At 4weeks, most of the LASC were positive for myogenic antigens including α-smooth muscle actin, desmin and calponin I. Conclusions: Periurethral injection of autologous LASC capable of myogenic differentiation made a greater contribution to the increase in urethral resistance than did the conventional collagen bulk injection. Thus, its use for treatment of stress urinary incontinence can be postulated.

Original languageEnglish
Pages (from-to)659-666
Number of pages8
JournalInternational Journal of Urology
Volume18
Issue number9
DOIs
Publication statusPublished - 01-09-2011
Externally publishedYes

Fingerprint

Mesenchymal Stromal Cells
Pressure
Collagen
Injections
Serum
Stress Urinary Incontinence
Cell Tracking
Nude Rats
Transgenic Rats
Desmin
Inbred F344 Rats
Cell- and Tissue-Based Therapy
Green Fluorescent Proteins
Smooth Muscle
Reflex
Adipose Tissue
Actins
Antigens
Control Groups
Therapeutics

All Science Journal Classification (ASJC) codes

  • Urology

Cite this

Watanabe, Tatsuhito ; Maruyama, Shoichi ; Yamamoto, Tokunori ; Kamo, Izumi ; Yasuda, Kaoru ; Saka, Yosuke ; Ozaki, Takenori ; Yuzawa, Yukio ; Matsuo, Seiichi ; Gotoh, Momokazu. / Increased urethral resistance by periurethral injection of low serum cultured adipose-derived mesenchymal stromal cells in rats. In: International Journal of Urology. 2011 ; Vol. 18, No. 9. pp. 659-666.
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Increased urethral resistance by periurethral injection of low serum cultured adipose-derived mesenchymal stromal cells in rats. / Watanabe, Tatsuhito; Maruyama, Shoichi; Yamamoto, Tokunori; Kamo, Izumi; Yasuda, Kaoru; Saka, Yosuke; Ozaki, Takenori; Yuzawa, Yukio; Matsuo, Seiichi; Gotoh, Momokazu.

In: International Journal of Urology, Vol. 18, No. 9, 01.09.2011, p. 659-666.

Research output: Contribution to journalArticle

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T1 - Increased urethral resistance by periurethral injection of low serum cultured adipose-derived mesenchymal stromal cells in rats

AU - Watanabe, Tatsuhito

AU - Maruyama, Shoichi

AU - Yamamoto, Tokunori

AU - Kamo, Izumi

AU - Yasuda, Kaoru

AU - Saka, Yosuke

AU - Ozaki, Takenori

AU - Yuzawa, Yukio

AU - Matsuo, Seiichi

AU - Gotoh, Momokazu

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Y1 - 2011/9/1

N2 - Objectives: To evaluate the effects of a periurethral injection of low serum cultured adipose tissue-derived mesenchymal stromal cells (LASC) and to develop a new autologous cell therapy for stress urinary incontinence. Methods: F344 rats were divided into three groups as based on the periurethral injection of LASC, GAX collagen or vehicle (control). At 2 and 4weeks after injection, leak point pressure (LPP) was measured before and after transection of the pelvic nerves. For cell tracking, LASC of green fluorescent protein transgenic rats were injected into nude rats. Results: At 2weeks, both the LASC and collagen groups showed significantly higher LPP than the control group. At 4weeks, the increase in LPP in the LASC group remained, whereas LPP in the collagen group decreased to baseline levels. In the absence of the urethral closure reflex after transection of the pelvic nerves, LPP in the LASC group was significantly higher than that in the other two groups. Histologically, the size of the urethral lumen was smaller in the LASC group than the collagen group. At 4weeks, most of the LASC were positive for myogenic antigens including α-smooth muscle actin, desmin and calponin I. Conclusions: Periurethral injection of autologous LASC capable of myogenic differentiation made a greater contribution to the increase in urethral resistance than did the conventional collagen bulk injection. Thus, its use for treatment of stress urinary incontinence can be postulated.

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