IncX2 and IncX1-X2 hybrid plasmids coexisting in a FosA6-producing Escherichia coli strain

Qinglan Guo, Jiachun Su, Christi Lee McElheny, Nicole Stoesser, Yohei Doi, Minggui Wang

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

IncX plasmids are receiving much attention as vehicles of carbapenem and colistin resistance genes, such as blaNDM, blaKPC, and mcr-1. Among them, IncX2 subgroup plasmids remain rare. Here, we characterized IncX2 and IncX1-X2 hybrid plasmids coexisting in a FosA6-producing Escherichia coli strain that were possibly generated as a consequence of recombination events between an R6K-like IncX2 plasmid and a pLN126_33-like IncX1 plasmid. Variable multidrug resistance mosaic regions were observed in these plasmids, indicating their potential to serve as flexible carriers of resistance genes. The diversity of IncX group plasmid backbones and accessory genes and the evolution of hybrid IncX plasmids pose a challenge in detecting and classifying them.

Original languageEnglish
Article numbere00536-17
JournalAntimicrobial agents and chemotherapy
Volume61
Issue number7
DOIs
Publication statusPublished - 01-07-2017

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Plasmids
Escherichia coli
Genes
Colistin
Carbapenems
Multiple Drug Resistance
Genetic Recombination

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

Cite this

Guo, Qinglan ; Su, Jiachun ; McElheny, Christi Lee ; Stoesser, Nicole ; Doi, Yohei ; Wang, Minggui. / IncX2 and IncX1-X2 hybrid plasmids coexisting in a FosA6-producing Escherichia coli strain. In: Antimicrobial agents and chemotherapy. 2017 ; Vol. 61, No. 7.
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IncX2 and IncX1-X2 hybrid plasmids coexisting in a FosA6-producing Escherichia coli strain. / Guo, Qinglan; Su, Jiachun; McElheny, Christi Lee; Stoesser, Nicole; Doi, Yohei; Wang, Minggui.

In: Antimicrobial agents and chemotherapy, Vol. 61, No. 7, e00536-17, 01.07.2017.

Research output: Contribution to journalArticle

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AU - Su, Jiachun

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AU - Doi, Yohei

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AB - IncX plasmids are receiving much attention as vehicles of carbapenem and colistin resistance genes, such as blaNDM, blaKPC, and mcr-1. Among them, IncX2 subgroup plasmids remain rare. Here, we characterized IncX2 and IncX1-X2 hybrid plasmids coexisting in a FosA6-producing Escherichia coli strain that were possibly generated as a consequence of recombination events between an R6K-like IncX2 plasmid and a pLN126_33-like IncX1 plasmid. Variable multidrug resistance mosaic regions were observed in these plasmids, indicating their potential to serve as flexible carriers of resistance genes. The diversity of IncX group plasmid backbones and accessory genes and the evolution of hybrid IncX plasmids pose a challenge in detecting and classifying them.

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