Independent evolution of multi-dominant viral genome species observed in a hepatitis C virus carrier

Tomomi Ando, Hideki Aizaki, Masaya Sugiyama, Tomoko Date, Kazuhiko Hayashi, Masatoshi Ishigami, Yoshiaki Katano, Hidemi Goto, Masashi Mizokami, Masamichi Muramatsu, Makoto Kuroda, Takaji Wakita

Research output: Contribution to journalArticlepeer-review


The viral genome quasispecies composition of hepatitis C virus (HCV) could have important implications to viral pathogenesis and resistance to anti-viral treatment. The purpose of the present study was to profile the HCV RNA quasispecies. We developed a strategy to determine the full-length HCV genome sequences co-existing within a single patient serum by using next-generation sequencing technologies. The isolated viral clones were divided into the groups that can be distinguished by core amino acid 70 substitution. Subsequently, we determined HCV full-length genome sequences of three independent dominant species co-existing in the sequential serum with a 7-year interval. From phylogenetic analysis, these dominant species evolved independently. Our study demonstrated that multiple dominant species co-existed in patient sera and evolved independently.

Original languageEnglish
Article number101327
JournalBiochemistry and Biophysics Reports
Publication statusPublished - 12-2022
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Cell Biology


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