TY - JOUR
T1 - Individual regulation of different hepatocellular functions during sepsis
AU - von Allmen, Daniel
AU - Hasselgren, Per Olof
AU - Higashiguchi, Takashi
AU - Fischer, Josef E.
N1 - Funding Information:
From the Department of Sueety, Universi~ of Cincinnati Medical Center, Cincinnati, OH. Supported in part by National Institutes of Health Grant No. IROI DK40644. Address reprint requests to Per-Olaf Hasselgren, MD, Department of Surgery, University of Cincinnati, 231 Bethesda Ave, Cincinnati, OH 45267-0558. Copyright 0 1992 by W.B . Saunders Company 0026-0495/92/4109-0006$03.00/0
PY - 1992/9
Y1 - 1992/9
N2 - The purpose of this study was to test the hypothesis that different hepatocellular functions are regulated individually during sepsis. This was done by simultaneously measuring bile production, release of liver transaminases, and synthesis of secreted proteins in perfused livers from control and septic rats. Sepsis was induced by cecal ligation and puncture (CLP); control rats were sham-operated. After 16 hours, livers were perfused in situ, and bile flow, synthesis rates of albumin and α1-acid glycoprotein (a major acute-phase protein in rats), and release of glutamic-oxaloacetic transaminase (GOT) and glutamicpyruvic transaminase (GPT) into perfusate were determined. Within the same livers, sepsis resulted in a 54% increase in the synthesis of α1-acid glycoprotein and approximately 30% inhibition of albumin synthesis concomitant with 50% lower bile flow. The concentrations of GOT and GPT in the perfusate increased slightly during the experiments, both when control and septic livers were perfused. The maintained tissue levels of adenosine triphosphate (ATP) and the uptake of Evans blue dye by less than 1% of the hepatocytes, although a late test of viability, suggest that both control and septic livers remained viable during perfusion. The results are consistent with the concept that different hepatocellular functions are individually regulated during sepsis. Thus, impairment of certain hepatocellular functions does not necessarily imply generalized liver failure.
AB - The purpose of this study was to test the hypothesis that different hepatocellular functions are regulated individually during sepsis. This was done by simultaneously measuring bile production, release of liver transaminases, and synthesis of secreted proteins in perfused livers from control and septic rats. Sepsis was induced by cecal ligation and puncture (CLP); control rats were sham-operated. After 16 hours, livers were perfused in situ, and bile flow, synthesis rates of albumin and α1-acid glycoprotein (a major acute-phase protein in rats), and release of glutamic-oxaloacetic transaminase (GOT) and glutamicpyruvic transaminase (GPT) into perfusate were determined. Within the same livers, sepsis resulted in a 54% increase in the synthesis of α1-acid glycoprotein and approximately 30% inhibition of albumin synthesis concomitant with 50% lower bile flow. The concentrations of GOT and GPT in the perfusate increased slightly during the experiments, both when control and septic livers were perfused. The maintained tissue levels of adenosine triphosphate (ATP) and the uptake of Evans blue dye by less than 1% of the hepatocytes, although a late test of viability, suggest that both control and septic livers remained viable during perfusion. The results are consistent with the concept that different hepatocellular functions are individually regulated during sepsis. Thus, impairment of certain hepatocellular functions does not necessarily imply generalized liver failure.
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U2 - 10.1016/0026-0495(92)90121-P
DO - 10.1016/0026-0495(92)90121-P
M3 - Article
C2 - 1518425
AN - SCOPUS:0026726619
SN - 0026-0495
VL - 41
SP - 961
EP - 965
JO - Metabolism
JF - Metabolism
IS - 9
ER -
