Abstract
Induced pluripotent stem cell (iPSC) technology offers a novel approach for conversion of human primary fibroblasts into melanocytes. During attempts to explore various protocols for differentiation of iPSCs into melanocytes, we found a distinct and self-renewing cell lineage that could differentiate into melanocytes, named as melanocyte precursor cells (MPCs). The MPCs exhibited a morphology distinctive from that of melanocytes, in lacking either the melanosomal structure or the melanocyte-specific marker genes MITF, TYR, and SOX10. In addition, gene expression studies in the MPCs showed high-level expression of WNT5A, ROR2, which are non-canonical WNT pathway markers, and its related receptor TGFβR2. In contrast, MPC differentiation into melanocytes was achieved by activating the canonical WNT pathway using the GSK3β inhibitor. Our data demonstrated the distinct characteristic of MPCs' ability to differentiate into melanocytes, and the underlying mechanism of interfacing between canonical WNT signaling pathway and non-canonical WNT signaling pathway.
| Original language | English |
|---|---|
| Pages (from-to) | 623-633 |
| Number of pages | 11 |
| Journal | Pigment Cell and Melanoma Research |
| Volume | 32 |
| Issue number | 5 |
| DOIs | |
| Publication status | Published - 01-01-2019 |
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All Science Journal Classification (ASJC) codes
- Oncology
- Biochemistry, Genetics and Molecular Biology(all)
- Dermatology
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Induced pluripotent stem cell-derived melanocyte precursor cells undergoing differentiation into melanocytes. / Hosaka, Chieko; Kunisada, Makoto; Koyanagi-Aoi, Michiyo; Masaki, Taro; Takemori, Chihiro; Ikeda, Mariko; Aoi, Takashi; Nishigori, Chikako.
In: Pigment Cell and Melanoma Research, Vol. 32, No. 5, 01.01.2019, p. 623-633.Research output: Contribution to journal › Article
TY - JOUR
T1 - Induced pluripotent stem cell-derived melanocyte precursor cells undergoing differentiation into melanocytes
AU - Hosaka, Chieko
AU - Kunisada, Makoto
AU - Koyanagi-Aoi, Michiyo
AU - Masaki, Taro
AU - Takemori, Chihiro
AU - Ikeda, Mariko
AU - Aoi, Takashi
AU - Nishigori, Chikako
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Induced pluripotent stem cell (iPSC) technology offers a novel approach for conversion of human primary fibroblasts into melanocytes. During attempts to explore various protocols for differentiation of iPSCs into melanocytes, we found a distinct and self-renewing cell lineage that could differentiate into melanocytes, named as melanocyte precursor cells (MPCs). The MPCs exhibited a morphology distinctive from that of melanocytes, in lacking either the melanosomal structure or the melanocyte-specific marker genes MITF, TYR, and SOX10. In addition, gene expression studies in the MPCs showed high-level expression of WNT5A, ROR2, which are non-canonical WNT pathway markers, and its related receptor TGFβR2. In contrast, MPC differentiation into melanocytes was achieved by activating the canonical WNT pathway using the GSK3β inhibitor. Our data demonstrated the distinct characteristic of MPCs' ability to differentiate into melanocytes, and the underlying mechanism of interfacing between canonical WNT signaling pathway and non-canonical WNT signaling pathway.
AB - Induced pluripotent stem cell (iPSC) technology offers a novel approach for conversion of human primary fibroblasts into melanocytes. During attempts to explore various protocols for differentiation of iPSCs into melanocytes, we found a distinct and self-renewing cell lineage that could differentiate into melanocytes, named as melanocyte precursor cells (MPCs). The MPCs exhibited a morphology distinctive from that of melanocytes, in lacking either the melanosomal structure or the melanocyte-specific marker genes MITF, TYR, and SOX10. In addition, gene expression studies in the MPCs showed high-level expression of WNT5A, ROR2, which are non-canonical WNT pathway markers, and its related receptor TGFβR2. In contrast, MPC differentiation into melanocytes was achieved by activating the canonical WNT pathway using the GSK3β inhibitor. Our data demonstrated the distinct characteristic of MPCs' ability to differentiate into melanocytes, and the underlying mechanism of interfacing between canonical WNT signaling pathway and non-canonical WNT signaling pathway.
UR - http://www.scopus.com/inward/record.url?scp=85063491797&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85063491797&partnerID=8YFLogxK
U2 - 10.1111/pcmr.12779
DO - 10.1111/pcmr.12779
M3 - Article
AN - SCOPUS:85063491797
VL - 32
SP - 623
EP - 633
JO - Pigment Cell and Melanoma Research
JF - Pigment Cell and Melanoma Research
SN - 1755-1471
IS - 5
ER -