TY - JOUR
T1 - Induced pluripotent stem cell-derived melanocyte precursor cells undergoing differentiation into melanocytes
AU - Hosaka, Chieko
AU - Kunisada, Makoto
AU - Koyanagi-Aoi, Michiyo
AU - Masaki, Taro
AU - Takemori, Chihiro
AU - Taniguchi-Ikeda, Mariko
AU - Aoi, Takashi
AU - Nishigori, Chikako
N1 - Funding Information:
This work was supported by Grant‐in‐Aid 16k10156 from the Ministry of Education, Culture, Sports, Science, and Technology of Japan (MEXT) (CN) and by the Practical Research Project for Rare/Intractable Diseases from the Japanese Agency for Medical Research and Development, AMED (15ek0109028h0002, 16ek0109028h003) (MK, TA and CA). We thank Mari Kohmoto for excellent technical advice and support.
Publisher Copyright:
© 2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd
PY - 2019
Y1 - 2019
N2 - Induced pluripotent stem cell (iPSC) technology offers a novel approach for conversion of human primary fibroblasts into melanocytes. During attempts to explore various protocols for differentiation of iPSCs into melanocytes, we found a distinct and self-renewing cell lineage that could differentiate into melanocytes, named as melanocyte precursor cells (MPCs). The MPCs exhibited a morphology distinctive from that of melanocytes, in lacking either the melanosomal structure or the melanocyte-specific marker genes MITF, TYR, and SOX10. In addition, gene expression studies in the MPCs showed high-level expression of WNT5A, ROR2, which are non-canonical WNT pathway markers, and its related receptor TGFβR2. In contrast, MPC differentiation into melanocytes was achieved by activating the canonical WNT pathway using the GSK3β inhibitor. Our data demonstrated the distinct characteristic of MPCs' ability to differentiate into melanocytes, and the underlying mechanism of interfacing between canonical WNT signaling pathway and non-canonical WNT signaling pathway.
AB - Induced pluripotent stem cell (iPSC) technology offers a novel approach for conversion of human primary fibroblasts into melanocytes. During attempts to explore various protocols for differentiation of iPSCs into melanocytes, we found a distinct and self-renewing cell lineage that could differentiate into melanocytes, named as melanocyte precursor cells (MPCs). The MPCs exhibited a morphology distinctive from that of melanocytes, in lacking either the melanosomal structure or the melanocyte-specific marker genes MITF, TYR, and SOX10. In addition, gene expression studies in the MPCs showed high-level expression of WNT5A, ROR2, which are non-canonical WNT pathway markers, and its related receptor TGFβR2. In contrast, MPC differentiation into melanocytes was achieved by activating the canonical WNT pathway using the GSK3β inhibitor. Our data demonstrated the distinct characteristic of MPCs' ability to differentiate into melanocytes, and the underlying mechanism of interfacing between canonical WNT signaling pathway and non-canonical WNT signaling pathway.
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U2 - 10.1111/pcmr.12779
DO - 10.1111/pcmr.12779
M3 - Article
C2 - 30843370
AN - SCOPUS:85063491797
VL - 32
SP - 623
EP - 633
JO - Pigment Cell and Melanoma Research
JF - Pigment Cell and Melanoma Research
SN - 1755-1471
IS - 5
ER -