TY - JOUR
T1 - Inducible-costimulator-mediated suppression of human immunodeficiency virus type 1 replication in CD4+ T lymphocytes
AU - Zhou, Xin
AU - Kubo, Makoto
AU - Nishitsuji, Hironori
AU - Kurihara, Kiyoshi
AU - Ikeda, Tamako
AU - Ohashi, Takashi
AU - Azuma, Miyuki
AU - Masuda, Takao
AU - Kannagi, Mari
N1 - Funding Information:
We thank Japan Tobacco Inc. (Osaka, Japan) for providing SA12 mAb. This work was supported by grants from the Ministry of Health, Labor, and Welfare of Japan.
PY - 2004/8/1
Y1 - 2004/8/1
N2 - We investigated the effects of signaling through CD28 family molecules on human immunodeficiency virus type 1 (HIV-1) replication in vitro. A monoclonal antibody (mAb) specific for inducible costimulator (ICOS) suppressed both X4 and R5 HIV-1 replication in CD4+ peripheral blood mononuclear cells (PBMC). This suppression was not attributable to reduced cell growth or viability. CD28 mAb showed variable effects and also suppressed HIV-1 replication when immobilized. Replication of pseudotype viruses with HIV-1-but not with vesicular stomatitis virus G-envelope was efficiently suppressed in CD4+ PBMC treated with ICOS or CD28 mAbs. However, CD4, CXCR4, and CCR5 expression on the surface was not down-regulated. Moreover, HIV-1 replication in CD4+ PBMC was suppressed by a soluble form of human B7-H2, a ligand of ICOS, but was enhanced by soluble B7-1, a ligand for CD28. These findings suggest that natural or artificial ligands for ICOS potentially suppress HIV-1 replication mainly at the entry stages.
AB - We investigated the effects of signaling through CD28 family molecules on human immunodeficiency virus type 1 (HIV-1) replication in vitro. A monoclonal antibody (mAb) specific for inducible costimulator (ICOS) suppressed both X4 and R5 HIV-1 replication in CD4+ peripheral blood mononuclear cells (PBMC). This suppression was not attributable to reduced cell growth or viability. CD28 mAb showed variable effects and also suppressed HIV-1 replication when immobilized. Replication of pseudotype viruses with HIV-1-but not with vesicular stomatitis virus G-envelope was efficiently suppressed in CD4+ PBMC treated with ICOS or CD28 mAbs. However, CD4, CXCR4, and CCR5 expression on the surface was not down-regulated. Moreover, HIV-1 replication in CD4+ PBMC was suppressed by a soluble form of human B7-H2, a ligand of ICOS, but was enhanced by soluble B7-1, a ligand for CD28. These findings suggest that natural or artificial ligands for ICOS potentially suppress HIV-1 replication mainly at the entry stages.
KW - CD28
KW - Costimulatory molecule
KW - HIV-1 suppression
KW - ICOS
KW - Immune regulation
KW - T cell activation
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U2 - 10.1016/j.virol.2004.04.037
DO - 10.1016/j.virol.2004.04.037
M3 - Article
C2 - 15246265
AN - SCOPUS:3142638815
SN - 0042-6822
VL - 325
SP - 252
EP - 263
JO - Virology
JF - Virology
IS - 2
ER -
