Induction of inducible nitric oxide synthase by ginsenosides in cultured porcine endothelial cells

Zhiyuan Li; Yasuharu Niwa; Sadaichi Sakamoto; Masayuki Shono; Xiu Chen; Yutaka Nakaya

doi:10.1016/S0024-3205(00)00880-8

Induction of inducible nitric oxide synthase by ginsenosides in cultured porcine endothelial cells

Zhiyuan Li, Yasuharu Niwa, Sadaichi Sakamoto, Masayuki Shono, Xiu Chen, Yutaka Nakaya

International Center for Cell and Gene Therapy

Research output: Contribution to journal › Article › peer-review

18 Citations (Scopus)

Abstract

Mechanism of Nitric oxide (NO) production by ginsenosides was investigated in cultured porcine endothelial cells. β-Nicotinamide adenine dinucleotide phosphate (β-NADPH) staining showed that the NO production was significantly enhanced by the presence of 40 μg/ml ginsenosides with 10 μM L-arginine after 12 h incubation. NO production was suppressed by addition of 0.5 μM N(ω)-Nitro-Larginine (L-NNA), an inhibitor of NO synthases (NOSs), to the incubation medium. In addition, the immunoreactive signals of inducible NOS (iNOS) were appeared in endothelial cells after 12-h incubation of ginsenosides, whereas the signals were not observed in non-treated cells. Our findings suggest that ginsenosides can enhance NO production by induction of iNOS in addition to its direct effect on endothelial cells by increasing intracellular Ca²⁺ concentration. (C) 2000 Elsevier Science Inc.

Original language	English
Pages (from-to)	2983-2989
Number of pages	7
Journal	Life Sciences
Volume	67
Issue number	24
DOIs	https://doi.org/10.1016/S0024-3205(00)00880-8
Publication status	Published - 03-11-2000

All Science Journal Classification (ASJC) codes

General Pharmacology, Toxicology and Pharmaceutics
General Biochemistry,Genetics and Molecular Biology

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10.1016/S0024-3205(00)00880-8

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title = "Induction of inducible nitric oxide synthase by ginsenosides in cultured porcine endothelial cells",

abstract = "Mechanism of Nitric oxide (NO) production by ginsenosides was investigated in cultured porcine endothelial cells. β-Nicotinamide adenine dinucleotide phosphate (β-NADPH) staining showed that the NO production was significantly enhanced by the presence of 40 μg/ml ginsenosides with 10 μM L-arginine after 12 h incubation. NO production was suppressed by addition of 0.5 μM N(ω)-Nitro-Larginine (L-NNA), an inhibitor of NO synthases (NOSs), to the incubation medium. In addition, the immunoreactive signals of inducible NOS (iNOS) were appeared in endothelial cells after 12-h incubation of ginsenosides, whereas the signals were not observed in non-treated cells. Our findings suggest that ginsenosides can enhance NO production by induction of iNOS in addition to its direct effect on endothelial cells by increasing intracellular Ca2+ concentration. (C) 2000 Elsevier Science Inc.",

author = "Zhiyuan Li and Yasuharu Niwa and Sadaichi Sakamoto and Masayuki Shono and Xiu Chen and Yutaka Nakaya",

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T1 - Induction of inducible nitric oxide synthase by ginsenosides in cultured porcine endothelial cells

AU - Li, Zhiyuan

AU - Niwa, Yasuharu

AU - Sakamoto, Sadaichi

AU - Shono, Masayuki

AU - Chen, Xiu

AU - Nakaya, Yutaka

PY - 2000/11/3

Y1 - 2000/11/3

N2 - Mechanism of Nitric oxide (NO) production by ginsenosides was investigated in cultured porcine endothelial cells. β-Nicotinamide adenine dinucleotide phosphate (β-NADPH) staining showed that the NO production was significantly enhanced by the presence of 40 μg/ml ginsenosides with 10 μM L-arginine after 12 h incubation. NO production was suppressed by addition of 0.5 μM N(ω)-Nitro-Larginine (L-NNA), an inhibitor of NO synthases (NOSs), to the incubation medium. In addition, the immunoreactive signals of inducible NOS (iNOS) were appeared in endothelial cells after 12-h incubation of ginsenosides, whereas the signals were not observed in non-treated cells. Our findings suggest that ginsenosides can enhance NO production by induction of iNOS in addition to its direct effect on endothelial cells by increasing intracellular Ca2+ concentration. (C) 2000 Elsevier Science Inc.

AB - Mechanism of Nitric oxide (NO) production by ginsenosides was investigated in cultured porcine endothelial cells. β-Nicotinamide adenine dinucleotide phosphate (β-NADPH) staining showed that the NO production was significantly enhanced by the presence of 40 μg/ml ginsenosides with 10 μM L-arginine after 12 h incubation. NO production was suppressed by addition of 0.5 μM N(ω)-Nitro-Larginine (L-NNA), an inhibitor of NO synthases (NOSs), to the incubation medium. In addition, the immunoreactive signals of inducible NOS (iNOS) were appeared in endothelial cells after 12-h incubation of ginsenosides, whereas the signals were not observed in non-treated cells. Our findings suggest that ginsenosides can enhance NO production by induction of iNOS in addition to its direct effect on endothelial cells by increasing intracellular Ca2+ concentration. (C) 2000 Elsevier Science Inc.

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ER -

Induction of inducible nitric oxide synthase by ginsenosides in cultured porcine endothelial cells

Abstract

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