Induction of inducible nitric oxide synthase by ginsenosides in cultured porcine endothelial cells

Zhiyuan Li, Yasuharu Niwa, Sadaichi Sakamoto, Masayuki Shono, Xiu Chen, Yutaka Nakaya

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

Mechanism of Nitric oxide (NO) production by ginsenosides was investigated in cultured porcine endothelial cells. β-Nicotinamide adenine dinucleotide phosphate (β-NADPH) staining showed that the NO production was significantly enhanced by the presence of 40 μg/ml ginsenosides with 10 μM L-arginine after 12 h incubation. NO production was suppressed by addition of 0.5 μM N(ω)-Nitro-Larginine (L-NNA), an inhibitor of NO synthases (NOSs), to the incubation medium. In addition, the immunoreactive signals of inducible NOS (iNOS) were appeared in endothelial cells after 12-h incubation of ginsenosides, whereas the signals were not observed in non-treated cells. Our findings suggest that ginsenosides can enhance NO production by induction of iNOS in addition to its direct effect on endothelial cells by increasing intracellular Ca2+ concentration. (C) 2000 Elsevier Science Inc.

Original languageEnglish
Pages (from-to)2983-2989
Number of pages7
JournalLife Sciences
Volume67
Issue number24
DOIs
Publication statusPublished - 03-11-2000
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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