We recently reported that male mice have fewer Langerhans' cells (LCs) than females and that orchiectomy resulted in an increased LC density. In this study we demonstrate that orchiectomy induces a transient increase in the number of paired LCs (PLCs) before the increase in LC density occurs. The results of double staining for adenosine triphosphatase (ATPase) and 5-bromo-2'-deoxyuridine (BrdU) showed that all examined PLCs had incorporated the intraperitoneally injected BrdU, while only 0.7% of the unpaired LCs were BrdU-positive; that supported the notion of PLCs as being divided daughter LCs. Orchiectomy appears to induce a transient increase in mitotic activity of LCs resulting in the increased LC density.
|Number of pages||4|
|Publication status||Published - 01-01-1990|
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