Induction of Langerhans' cell mitosis in vivo after orchiectomy on mice

Y. I. Koyama, S. Nagao, K. Ohashi, H. Takahashi, T. Marunouchi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We recently reported that male mice have fewer Langerhans' cells (LCs) than females and that orchiectomy resulted in an increased LC density. In this study we demonstrate that orchiectomy induces a transient increase in the number of paired LCs (PLCs) before the increase in LC density occurs. The results of double staining for adenosine triphosphatase (ATPase) and 5-bromo-2'-deoxyuridine (BrdU) showed that all examined PLCs had incorporated the intraperitoneally injected BrdU, while only 0.7% of the unpaired LCs were BrdU-positive; that supported the notion of PLCs as being divided daughter LCs. Orchiectomy appears to induce a transient increase in mitotic activity of LCs resulting in the increased LC density.

Original languageEnglish
Pages (from-to)505-508
Number of pages4
JournalActa Dermato-Venereologica
Volume70
Issue number6
Publication statusPublished - 01-01-1990

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Orchiectomy
Langerhans Cells
Mitosis
Bromodeoxyuridine
Cell Count
Adenosine Triphosphatases
Staining and Labeling

All Science Journal Classification (ASJC) codes

  • Dermatology

Cite this

Koyama, Y. I. ; Nagao, S. ; Ohashi, K. ; Takahashi, H. ; Marunouchi, T. / Induction of Langerhans' cell mitosis in vivo after orchiectomy on mice. In: Acta Dermato-Venereologica. 1990 ; Vol. 70, No. 6. pp. 505-508.
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Koyama, YI, Nagao, S, Ohashi, K, Takahashi, H & Marunouchi, T 1990, 'Induction of Langerhans' cell mitosis in vivo after orchiectomy on mice', Acta Dermato-Venereologica, vol. 70, no. 6, pp. 505-508.

Induction of Langerhans' cell mitosis in vivo after orchiectomy on mice. / Koyama, Y. I.; Nagao, S.; Ohashi, K.; Takahashi, H.; Marunouchi, T.

In: Acta Dermato-Venereologica, Vol. 70, No. 6, 01.01.1990, p. 505-508.

Research output: Contribution to journalArticle

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AB - We recently reported that male mice have fewer Langerhans' cells (LCs) than females and that orchiectomy resulted in an increased LC density. In this study we demonstrate that orchiectomy induces a transient increase in the number of paired LCs (PLCs) before the increase in LC density occurs. The results of double staining for adenosine triphosphatase (ATPase) and 5-bromo-2'-deoxyuridine (BrdU) showed that all examined PLCs had incorporated the intraperitoneally injected BrdU, while only 0.7% of the unpaired LCs were BrdU-positive; that supported the notion of PLCs as being divided daughter LCs. Orchiectomy appears to induce a transient increase in mitotic activity of LCs resulting in the increased LC density.

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