Induction of neuronal axon outgrowth by Shati/Nat8l by energy metabolism in mice cultured neurons

Kazuyuki Sumi, Kyosuke Uno, Shohei Matsumura, Yoshiaki Miyamoto, Yoko Furukawa-Hibi, Shin Ichi Muramatsu, Toshitaka Nabeshima, Atsumi Nitta

Research output: Contribution to journalArticle

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Abstract

A novel N-acetyltransferase, Shati/Nat8l, was identified in the nucleus accumbens of mice repeatedly treated with methamphetamine (METH). Shati/Nat8l has been reported to inhibit the pharmacological action induced by METH. Shati/Nat8l produces N-acetylaspartate from aspartate and acetyl-CoA. Previously, we reported that overexpression of Shati/Nat8l in nucleus accumbens attenuates the response to METH by N-acetylaspartylglutamate (which is derived from N-acetylaspartate)-mGluR3 signaling in the mice brain. In the present study, to clarify the type of cells that produce Shati/Nat8l, we carried out in-situ hybridization for the detection of Shati/Nat8l mRNA along with immunohistochemical studies using serial sections of mice brain. Shati/Nat8l mRNA was detected in neuronal cells, but not in astrocytes or microglia cells. Next, we investigated the function of Shati/Nat8l in the neuronal cells in mice brain; then, we used an adeno-associated virus vector containing Shati/Nat8l for transfection and overexpression of Shati/Nat8l protein into the primary cultured neurons to investigate the contribution toward the neuronal activity of Shati/Nat8l. Overexpression of Shati/Nat8l in the mice primary cultured neurons induced axonal growth, but not dendrite elongation at day 1.5 (DIV). This finding indicated that Shati/Nat8l contributes toward neuronal development. LY341495, a selective group II mGluRs antagonist, did not abolish this axonal growth, and N-acetylaspartylglutamate itself did not abolish axon outgrowth in the same cultured system. The cultured neurons overexpressing Shati/Nat8l contained high ATP, suggesting that axon outgrowth is dependent on energy metabolism. This study shows that Shati/Nat8l in the neuron may induce axon outgrowth by ATP synthesis and not through mGluR3 signaling.

Original languageEnglish
Pages (from-to)740-746
Number of pages7
JournalNeuroReport
Volume26
Issue number13
DOIs
Publication statusPublished - 01-01-2015
Externally publishedYes

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Energy Metabolism
Methamphetamine
Neurons
Nucleus Accumbens
LY 341495
Brain
Adenosine Triphosphate
Dependovirus
Messenger RNA
Acetyl Coenzyme A
Acetyltransferases
Microglia
Growth
Dendrites
Aspartic Acid
Astrocytes
In Situ Hybridization
Transfection
Neuronal Outgrowth
Pharmacology

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Sumi, K., Uno, K., Matsumura, S., Miyamoto, Y., Furukawa-Hibi, Y., Muramatsu, S. I., ... Nitta, A. (2015). Induction of neuronal axon outgrowth by Shati/Nat8l by energy metabolism in mice cultured neurons. NeuroReport, 26(13), 740-746. https://doi.org/10.1097/WNR.0000000000000416
Sumi, Kazuyuki ; Uno, Kyosuke ; Matsumura, Shohei ; Miyamoto, Yoshiaki ; Furukawa-Hibi, Yoko ; Muramatsu, Shin Ichi ; Nabeshima, Toshitaka ; Nitta, Atsumi. / Induction of neuronal axon outgrowth by Shati/Nat8l by energy metabolism in mice cultured neurons. In: NeuroReport. 2015 ; Vol. 26, No. 13. pp. 740-746.
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Sumi, K, Uno, K, Matsumura, S, Miyamoto, Y, Furukawa-Hibi, Y, Muramatsu, SI, Nabeshima, T & Nitta, A 2015, 'Induction of neuronal axon outgrowth by Shati/Nat8l by energy metabolism in mice cultured neurons', NeuroReport, vol. 26, no. 13, pp. 740-746. https://doi.org/10.1097/WNR.0000000000000416

Induction of neuronal axon outgrowth by Shati/Nat8l by energy metabolism in mice cultured neurons. / Sumi, Kazuyuki; Uno, Kyosuke; Matsumura, Shohei; Miyamoto, Yoshiaki; Furukawa-Hibi, Yoko; Muramatsu, Shin Ichi; Nabeshima, Toshitaka; Nitta, Atsumi.

In: NeuroReport, Vol. 26, No. 13, 01.01.2015, p. 740-746.

Research output: Contribution to journalArticle

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AU - Sumi, Kazuyuki

AU - Uno, Kyosuke

AU - Matsumura, Shohei

AU - Miyamoto, Yoshiaki

AU - Furukawa-Hibi, Yoko

AU - Muramatsu, Shin Ichi

AU - Nabeshima, Toshitaka

AU - Nitta, Atsumi

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Sumi K, Uno K, Matsumura S, Miyamoto Y, Furukawa-Hibi Y, Muramatsu SI et al. Induction of neuronal axon outgrowth by Shati/Nat8l by energy metabolism in mice cultured neurons. NeuroReport. 2015 Jan 1;26(13):740-746. https://doi.org/10.1097/WNR.0000000000000416