Induction of phosphorylated-Stat3 following focal cerebral ischemia in mice

Tong Chun Wen, Hui Peng, Ryuji Hata, Junzo Desaki, Masahiro Sakanaka

Research output: Contribution to journalArticle

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Abstract

It has been shown that Stat3 is induced following transient cerebral ischemia in rat. However there is no evidence that cerebral ischemia stimulates the expression of phosphorylated-Stat3 (p-Stat3), which can activate cytokine-mediated signal transduction from the membrane to the nucleus. In the present study, we investigated the changes in p-Stat3 expression following middle cerebral artery occlusion in mice. Western blot analysis revealed a significant increase in the p-Stat3 protein in the peripheral part of the ischemic area, starting from 6 h after ischemia. p-Stat3 immunoreactivity was detected only in neurons, but not in astrocytes or microglia, and p-Stat3-positive neurons were increased in number in the peripheral part of the ischemic area at 24 h after ischemia. Double staining with aTdT-mediated biotinylated UTP nick end labeling (TUNEL) kit and the p-Stat3 antibody indicated that p-Stat3-positive neurons were also TUNEL-positive. Subsequent immuno-electron microscopic observations showed that p-Stat3-positive neurons were at different stages of degeneration. The present findings suggest that the increased expression of p-Stat3 after cerebral ischemia could play a crucial role in ischemia-induced neuron death.

Original languageEnglish
Pages (from-to)153-156
Number of pages4
JournalNeuroscience Letters
Volume303
Issue number3
DOIs
Publication statusPublished - 11-05-2001

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Brain Ischemia
Neurons
Ischemia
In Situ Nick-End Labeling
Uridine Triphosphate
Middle Cerebral Artery Infarction
Transient Ischemic Attack
Microglia
Astrocytes
Signal Transduction
Western Blotting
Electrons
Staining and Labeling
Cytokines
Membranes
Antibodies
Proteins

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

Cite this

Wen, Tong Chun ; Peng, Hui ; Hata, Ryuji ; Desaki, Junzo ; Sakanaka, Masahiro. / Induction of phosphorylated-Stat3 following focal cerebral ischemia in mice. In: Neuroscience Letters. 2001 ; Vol. 303, No. 3. pp. 153-156.
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Induction of phosphorylated-Stat3 following focal cerebral ischemia in mice. / Wen, Tong Chun; Peng, Hui; Hata, Ryuji; Desaki, Junzo; Sakanaka, Masahiro.

In: Neuroscience Letters, Vol. 303, No. 3, 11.05.2001, p. 153-156.

Research output: Contribution to journalArticle

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AB - It has been shown that Stat3 is induced following transient cerebral ischemia in rat. However there is no evidence that cerebral ischemia stimulates the expression of phosphorylated-Stat3 (p-Stat3), which can activate cytokine-mediated signal transduction from the membrane to the nucleus. In the present study, we investigated the changes in p-Stat3 expression following middle cerebral artery occlusion in mice. Western blot analysis revealed a significant increase in the p-Stat3 protein in the peripheral part of the ischemic area, starting from 6 h after ischemia. p-Stat3 immunoreactivity was detected only in neurons, but not in astrocytes or microglia, and p-Stat3-positive neurons were increased in number in the peripheral part of the ischemic area at 24 h after ischemia. Double staining with aTdT-mediated biotinylated UTP nick end labeling (TUNEL) kit and the p-Stat3 antibody indicated that p-Stat3-positive neurons were also TUNEL-positive. Subsequent immuno-electron microscopic observations showed that p-Stat3-positive neurons were at different stages of degeneration. The present findings suggest that the increased expression of p-Stat3 after cerebral ischemia could play a crucial role in ischemia-induced neuron death.

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