TY - JOUR
T1 - Induction of trophinin in human endometrial surface epithelia by CGβ and IL-1β
AU - Sugihara, Kazuhiro
AU - Kabir-Salmani, Maryam
AU - Byrne, James
AU - Wolf, Don P.
AU - Lessey, Bruce
AU - Iwashita, Mitsutoshi
AU - Aoki, Daisuke
AU - Nakayama, Jun
AU - Fukuda, Michiko N.
N1 - Funding Information:
This study was supported by National Institutes of Health Grant HD34108 and Department of Defense Grant W81XWH-04-1-0917x (to M.N.F.).
PY - 2008/1/23
Y1 - 2008/1/23
N2 - During embryo implantation, trophinin mediates cell adhesion by homophilic binding at the apical surfaces of trophectoderm and endometrium. Trophinin is expressed on the human endometrial epithelia in rare occasions. We developed hCG-coated agarose beads that mimic the physical and physiological features of an implantation-stage human blastocyst. When hCG-coated beads were applied to human endometrial epithelial cells in the presence of IL-1β, endometrial cells acquired strong trophinin expression and the ability for apical cell adhesion with trophinin-expressing human trophoblastic cells. These results provide a mechanism for trophinin-mediated adhesion of human blastocyst to endometrium by a spatially and temporally restricted paracrine effect of hCG derived from the blastocyst.
AB - During embryo implantation, trophinin mediates cell adhesion by homophilic binding at the apical surfaces of trophectoderm and endometrium. Trophinin is expressed on the human endometrial epithelia in rare occasions. We developed hCG-coated agarose beads that mimic the physical and physiological features of an implantation-stage human blastocyst. When hCG-coated beads were applied to human endometrial epithelial cells in the presence of IL-1β, endometrial cells acquired strong trophinin expression and the ability for apical cell adhesion with trophinin-expressing human trophoblastic cells. These results provide a mechanism for trophinin-mediated adhesion of human blastocyst to endometrium by a spatially and temporally restricted paracrine effect of hCG derived from the blastocyst.
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U2 - 10.1016/j.febslet.2007.12.001
DO - 10.1016/j.febslet.2007.12.001
M3 - Article
C2 - 18078818
AN - SCOPUS:38049015486
SN - 0014-5793
VL - 582
SP - 197
EP - 202
JO - FEBS Letters
JF - FEBS Letters
IS - 2
ER -