TY - JOUR
T1 - Induction of tyrosine phosphorylation in human B lineage cells by crosslinking MB-1 molecule of B cell receptor-related heterodimer complex
AU - Kuwahara, Kazuhiko
AU - Igarashi, Hideya
AU - Kawai, Taro
AU - Ichigi, Yasuhisa
AU - Muraguchi, Atsushi
AU - Mason, David Y.
AU - Kimoto, Masao
AU - Inui, Seiji
AU - Sakaguchi, Nobuo
PY - 1993/12/30
Y1 - 1993/12/30
N2 - B cell antigen receptor is composed of immunoglobulin and associated MB-1 and B29. Here, we found that anti-human MB-1 stimulation induced tyrosine phosphorylation in immature B cells (FL4.4 and Nalm-6) but not in mature B cells (Daudi). Coprecipitated complex with the heterodimer component in Daudi and Nalm-6 contained the kinase molecule(s) which act on the heterodimer protein, while the complex in early lymphoid cell with germ line antigen receptor genes (FL4.4) did not. Candidate Fyn and Lyn are expressed in Nalm-6 and Daudi but are not expressed in FL4.4. These results suggested that src-type tyrosine kinases as Fyn and Lyn are responsible for the phosphorylation of MB-1 and B29 heterodimer, but anti-MB-1 stimulation can induce tyrosine phosphorylation reaction mediated by other kinase molecule(s) in the progenitor type cells.
AB - B cell antigen receptor is composed of immunoglobulin and associated MB-1 and B29. Here, we found that anti-human MB-1 stimulation induced tyrosine phosphorylation in immature B cells (FL4.4 and Nalm-6) but not in mature B cells (Daudi). Coprecipitated complex with the heterodimer component in Daudi and Nalm-6 contained the kinase molecule(s) which act on the heterodimer protein, while the complex in early lymphoid cell with germ line antigen receptor genes (FL4.4) did not. Candidate Fyn and Lyn are expressed in Nalm-6 and Daudi but are not expressed in FL4.4. These results suggested that src-type tyrosine kinases as Fyn and Lyn are responsible for the phosphorylation of MB-1 and B29 heterodimer, but anti-MB-1 stimulation can induce tyrosine phosphorylation reaction mediated by other kinase molecule(s) in the progenitor type cells.
UR - http://www.scopus.com/inward/record.url?scp=0027715464&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0027715464&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1993.2656
DO - 10.1006/bbrc.1993.2656
M3 - Article
C2 - 7506545
AN - SCOPUS:0027715464
VL - 197
SP - 1563
EP - 1569
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -