Induction of tyrosine phosphorylation in human B lineage cells by crosslinking MB-1 molecule of B cell receptor-related heterodimer complex

Kazuhiko Kuwahara, Hideya Igarashi, Taro Kawai, Yasuhisa Ichigi, Atsushi Muraguchi, David Y. Mason, Masao Kimoto, Seiji Inui, Nobuo Sakaguchi

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

B cell antigen receptor is composed of immunoglobulin and associated MB-1 and B29. Here, we found that anti-human MB-1 stimulation induced tyrosine phosphorylation in immature B cells (FL4.4 and Nalm-6) but not in mature B cells (Daudi). Coprecipitated complex with the heterodimer component in Daudi and Nalm-6 contained the kinase molecule(s) which act on the heterodimer protein, while the complex in early lymphoid cell with germ line antigen receptor genes (FL4.4) did not. Candidate Fyn and Lyn are expressed in Nalm-6 and Daudi but are not expressed in FL4.4. These results suggested that src-type tyrosine kinases as Fyn and Lyn are responsible for the phosphorylation of MB-1 and B29 heterodimer, but anti-MB-1 stimulation can induce tyrosine phosphorylation reaction mediated by other kinase molecule(s) in the progenitor type cells.

Original languageEnglish
Pages (from-to)1563-1569
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume197
Issue number3
DOIs
Publication statusPublished - 30-12-1993
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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