Induction of tyrosine phosphorylation in human B lineage cells by crosslinking MB-1 molecule of B cell receptor-related heterodimer complex

Kazuhiko Kuwahara; Hideya Igarashi; Taro Kawai; Yasuhisa Ichigi; Atsushi Muraguchi; David Y. Mason; Masao Kimoto; Seiji Inui; Nobuo Sakaguchi

doi:10.1006/bbrc.1993.2656

Induction of tyrosine phosphorylation in human B lineage cells by crosslinking MB-1 molecule of B cell receptor-related heterodimer complex

Kazuhiko Kuwahara, Hideya Igarashi, Taro Kawai, Yasuhisa Ichigi, Atsushi Muraguchi, David Y. Mason, Masao Kimoto, Seiji Inui, Nobuo Sakaguchi

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

B cell antigen receptor is composed of immunoglobulin and associated MB-1 and B29. Here, we found that anti-human MB-1 stimulation induced tyrosine phosphorylation in immature B cells (FL4.4 and Nalm-6) but not in mature B cells (Daudi). Coprecipitated complex with the heterodimer component in Daudi and Nalm-6 contained the kinase molecule(s) which act on the heterodimer protein, while the complex in early lymphoid cell with germ line antigen receptor genes (FL4.4) did not. Candidate Fyn and Lyn are expressed in Nalm-6 and Daudi but are not expressed in FL4.4. These results suggested that src-type tyrosine kinases as Fyn and Lyn are responsible for the phosphorylation of MB-1 and B29 heterodimer, but anti-MB-1 stimulation can induce tyrosine phosphorylation reaction mediated by other kinase molecule(s) in the progenitor type cells.

Original language	English
Pages (from-to)	1563-1569
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	197
Issue number	3
DOIs	https://doi.org/10.1006/bbrc.1993.2656
Publication status	Published - 30-12-1993
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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Kuwahara, K., Igarashi, H., Kawai, T., Ichigi, Y., Muraguchi, A., Mason, D. Y., Kimoto, M., Inui, S., & Sakaguchi, N. (1993). Induction of tyrosine phosphorylation in human B lineage cells by crosslinking MB-1 molecule of B cell receptor-related heterodimer complex. Biochemical and Biophysical Research Communications, 197(3), 1563-1569. https://doi.org/10.1006/bbrc.1993.2656

Kuwahara, Kazuhiko ; Igarashi, Hideya ; Kawai, Taro ; Ichigi, Yasuhisa ; Muraguchi, Atsushi ; Mason, David Y. ; Kimoto, Masao ; Inui, Seiji ; Sakaguchi, Nobuo. / Induction of tyrosine phosphorylation in human B lineage cells by crosslinking MB-1 molecule of B cell receptor-related heterodimer complex. In: Biochemical and Biophysical Research Communications. 1993 ; Vol. 197, No. 3. pp. 1563-1569.

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title = "Induction of tyrosine phosphorylation in human B lineage cells by crosslinking MB-1 molecule of B cell receptor-related heterodimer complex",

abstract = "B cell antigen receptor is composed of immunoglobulin and associated MB-1 and B29. Here, we found that anti-human MB-1 stimulation induced tyrosine phosphorylation in immature B cells (FL4.4 and Nalm-6) but not in mature B cells (Daudi). Coprecipitated complex with the heterodimer component in Daudi and Nalm-6 contained the kinase molecule(s) which act on the heterodimer protein, while the complex in early lymphoid cell with germ line antigen receptor genes (FL4.4) did not. Candidate Fyn and Lyn are expressed in Nalm-6 and Daudi but are not expressed in FL4.4. These results suggested that src-type tyrosine kinases as Fyn and Lyn are responsible for the phosphorylation of MB-1 and B29 heterodimer, but anti-MB-1 stimulation can induce tyrosine phosphorylation reaction mediated by other kinase molecule(s) in the progenitor type cells.",

author = "Kazuhiko Kuwahara and Hideya Igarashi and Taro Kawai and Yasuhisa Ichigi and Atsushi Muraguchi and Mason, {David Y.} and Masao Kimoto and Seiji Inui and Nobuo Sakaguchi",

year = "1993",

month = dec,

day = "30",

doi = "10.1006/bbrc.1993.2656",

language = "English",

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Kuwahara, K, Igarashi, H, Kawai, T, Ichigi, Y, Muraguchi, A, Mason, DY, Kimoto, M, Inui, S & Sakaguchi, N 1993, 'Induction of tyrosine phosphorylation in human B lineage cells by crosslinking MB-1 molecule of B cell receptor-related heterodimer complex', Biochemical and Biophysical Research Communications, vol. 197, no. 3, pp. 1563-1569. https://doi.org/10.1006/bbrc.1993.2656

Induction of tyrosine phosphorylation in human B lineage cells by crosslinking MB-1 molecule of B cell receptor-related heterodimer complex. / Kuwahara, Kazuhiko; Igarashi, Hideya; Kawai, Taro; Ichigi, Yasuhisa; Muraguchi, Atsushi; Mason, David Y.; Kimoto, Masao; Inui, Seiji; Sakaguchi, Nobuo.

In: Biochemical and Biophysical Research Communications, Vol. 197, No. 3, 30.12.1993, p. 1563-1569.

Research output: Contribution to journal › Article › peer-review

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AU - Kuwahara, Kazuhiko

AU - Igarashi, Hideya

AU - Kawai, Taro

AU - Ichigi, Yasuhisa

AU - Muraguchi, Atsushi

AU - Mason, David Y.

AU - Kimoto, Masao

AU - Inui, Seiji

AU - Sakaguchi, Nobuo

PY - 1993/12/30

Y1 - 1993/12/30

N2 - B cell antigen receptor is composed of immunoglobulin and associated MB-1 and B29. Here, we found that anti-human MB-1 stimulation induced tyrosine phosphorylation in immature B cells (FL4.4 and Nalm-6) but not in mature B cells (Daudi). Coprecipitated complex with the heterodimer component in Daudi and Nalm-6 contained the kinase molecule(s) which act on the heterodimer protein, while the complex in early lymphoid cell with germ line antigen receptor genes (FL4.4) did not. Candidate Fyn and Lyn are expressed in Nalm-6 and Daudi but are not expressed in FL4.4. These results suggested that src-type tyrosine kinases as Fyn and Lyn are responsible for the phosphorylation of MB-1 and B29 heterodimer, but anti-MB-1 stimulation can induce tyrosine phosphorylation reaction mediated by other kinase molecule(s) in the progenitor type cells.

AB - B cell antigen receptor is composed of immunoglobulin and associated MB-1 and B29. Here, we found that anti-human MB-1 stimulation induced tyrosine phosphorylation in immature B cells (FL4.4 and Nalm-6) but not in mature B cells (Daudi). Coprecipitated complex with the heterodimer component in Daudi and Nalm-6 contained the kinase molecule(s) which act on the heterodimer protein, while the complex in early lymphoid cell with germ line antigen receptor genes (FL4.4) did not. Candidate Fyn and Lyn are expressed in Nalm-6 and Daudi but are not expressed in FL4.4. These results suggested that src-type tyrosine kinases as Fyn and Lyn are responsible for the phosphorylation of MB-1 and B29 heterodimer, but anti-MB-1 stimulation can induce tyrosine phosphorylation reaction mediated by other kinase molecule(s) in the progenitor type cells.

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Induction of tyrosine phosphorylation in human B lineage cells by crosslinking MB-1 molecule of B cell receptor-related heterodimer complex

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