TY - JOUR
T1 - Inflammatory changes in adipose tissue enhance expression of GPR84, a medium-chain fatty acid receptor
T2 - TNFα enhances GPR84 expression in adipocytes
AU - Nagasaki, Hiroshi
AU - Kondo, Takaaki
AU - Fuchigami, Masahiro
AU - Hashimoto, Hiroyuki
AU - Sugimura, Yoshihisa
AU - Ozaki, Nobuaki
AU - Arima, Hiroshi
AU - Ota, Akira
AU - Oiso, Yutaka
AU - Hamada, Yoji
N1 - Funding Information:
This study was partially funded by grant-in-aid (No. 22500331) from the Ministry of Education, Science and Culture of Japan. The authors acknowledge Ms. Michiko Yamada, Nagoya University, for her excellent technical assistance. We also thank Dr. Larry Frye of BIOMEDCOM for the help in the preparation of this manuscript. There are no conflicts of interest regarding this study.
PY - 2012/2/17
Y1 - 2012/2/17
N2 - In this study we aimed to identify the physiological roles of G protein-coupled receptor 84 (GPR84) in adipose tissue, together with medium-chain fatty acids (MCFAs), the specific ligands for GPR84. In mice, high-fat diet up-regulated GPR84 expression in fat pads. In 3T3-L1 adipocytes, co-culture with a macrophage cell line, RAW264, or TNFα remarkably enhanced GPR84 expression. In the presence of TNFα, MCFAs down-regulated adiponectin mRNA expression in 3T3-L1 adipocytes. Taken together, our results suggest that GPR84 emerges in adipocytes in response to TNFα from infiltrating macrophages and exacerbates the vicious cycle between adiposity and diabesity.
AB - In this study we aimed to identify the physiological roles of G protein-coupled receptor 84 (GPR84) in adipose tissue, together with medium-chain fatty acids (MCFAs), the specific ligands for GPR84. In mice, high-fat diet up-regulated GPR84 expression in fat pads. In 3T3-L1 adipocytes, co-culture with a macrophage cell line, RAW264, or TNFα remarkably enhanced GPR84 expression. In the presence of TNFα, MCFAs down-regulated adiponectin mRNA expression in 3T3-L1 adipocytes. Taken together, our results suggest that GPR84 emerges in adipocytes in response to TNFα from infiltrating macrophages and exacerbates the vicious cycle between adiposity and diabesity.
UR - http://www.scopus.com/inward/record.url?scp=84857051975&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84857051975&partnerID=8YFLogxK
U2 - 10.1016/j.febslet.2012.01.001
DO - 10.1016/j.febslet.2012.01.001
M3 - Article
C2 - 22245676
AN - SCOPUS:84857051975
VL - 586
SP - 368
EP - 372
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 4
ER -