Inflammatory changes in adipose tissue enhance expression of GPR84, a medium-chain fatty acid receptor: TNFα enhances GPR84 expression in adipocytes

Hiroshi Nagasaki; Takaaki Kondo; Masahiro Fuchigami; Hiroyuki Hashimoto; Yoshihisa Sugimura; Nobuaki Ozaki; Hiroshi Arima; Akira Ota; Yutaka Oiso; Yoji Hamada

doi:10.1016/j.febslet.2012.01.001

Inflammatory changes in adipose tissue enhance expression of GPR84, a medium-chain fatty acid receptor: TNFα enhances GPR84 expression in adipocytes

Hiroshi Nagasaki, Takaaki Kondo, Masahiro Fuchigami, Hiroyuki Hashimoto, Yoshihisa Sugimura, Nobuaki Ozaki, Hiroshi Arima, Akira Ota, Yutaka Oiso, Yoji Hamada

Research output: Contribution to journal › Article › peer-review

83 Citations (Scopus)

Abstract

In this study we aimed to identify the physiological roles of G protein-coupled receptor 84 (GPR84) in adipose tissue, together with medium-chain fatty acids (MCFAs), the specific ligands for GPR84. In mice, high-fat diet up-regulated GPR84 expression in fat pads. In 3T3-L1 adipocytes, co-culture with a macrophage cell line, RAW264, or TNFα remarkably enhanced GPR84 expression. In the presence of TNFα, MCFAs down-regulated adiponectin mRNA expression in 3T3-L1 adipocytes. Taken together, our results suggest that GPR84 emerges in adipocytes in response to TNFα from infiltrating macrophages and exacerbates the vicious cycle between adiposity and diabesity.

Original language	English
Pages (from-to)	368-372
Number of pages	5
Journal	FEBS Letters
Volume	586
Issue number	4
DOIs	https://doi.org/10.1016/j.febslet.2012.01.001
Publication status	Published - 17-02-2012
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2012.01.001

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title = "Inflammatory changes in adipose tissue enhance expression of GPR84, a medium-chain fatty acid receptor: TNFα enhances GPR84 expression in adipocytes",

abstract = "In this study we aimed to identify the physiological roles of G protein-coupled receptor 84 (GPR84) in adipose tissue, together with medium-chain fatty acids (MCFAs), the specific ligands for GPR84. In mice, high-fat diet up-regulated GPR84 expression in fat pads. In 3T3-L1 adipocytes, co-culture with a macrophage cell line, RAW264, or TNFα remarkably enhanced GPR84 expression. In the presence of TNFα, MCFAs down-regulated adiponectin mRNA expression in 3T3-L1 adipocytes. Taken together, our results suggest that GPR84 emerges in adipocytes in response to TNFα from infiltrating macrophages and exacerbates the vicious cycle between adiposity and diabesity.",

keywords = "Adiponectin, Adipose tissue, GPR84, Medium-chain fatty acid, TNFα",

author = "Hiroshi Nagasaki and Takaaki Kondo and Masahiro Fuchigami and Hiroyuki Hashimoto and Yoshihisa Sugimura and Nobuaki Ozaki and Hiroshi Arima and Akira Ota and Yutaka Oiso and Yoji Hamada",

note = "Funding Information: This study was partially funded by grant-in-aid (No. 22500331) from the Ministry of Education, Science and Culture of Japan. The authors acknowledge Ms. Michiko Yamada, Nagoya University, for her excellent technical assistance. We also thank Dr. Larry Frye of BIOMEDCOM for the help in the preparation of this manuscript. There are no conflicts of interest regarding this study. ",

year = "2012",

month = feb,

day = "17",

doi = "10.1016/j.febslet.2012.01.001",

language = "English",

volume = "586",

pages = "368--372",

journal = "FEBS Letters",

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TY - JOUR

T1 - Inflammatory changes in adipose tissue enhance expression of GPR84, a medium-chain fatty acid receptor

T2 - TNFα enhances GPR84 expression in adipocytes

AU - Nagasaki, Hiroshi

AU - Kondo, Takaaki

AU - Fuchigami, Masahiro

AU - Hashimoto, Hiroyuki

AU - Sugimura, Yoshihisa

AU - Ozaki, Nobuaki

AU - Arima, Hiroshi

AU - Ota, Akira

AU - Oiso, Yutaka

AU - Hamada, Yoji

N1 - Funding Information: This study was partially funded by grant-in-aid (No. 22500331) from the Ministry of Education, Science and Culture of Japan. The authors acknowledge Ms. Michiko Yamada, Nagoya University, for her excellent technical assistance. We also thank Dr. Larry Frye of BIOMEDCOM for the help in the preparation of this manuscript. There are no conflicts of interest regarding this study.

PY - 2012/2/17

Y1 - 2012/2/17

N2 - In this study we aimed to identify the physiological roles of G protein-coupled receptor 84 (GPR84) in adipose tissue, together with medium-chain fatty acids (MCFAs), the specific ligands for GPR84. In mice, high-fat diet up-regulated GPR84 expression in fat pads. In 3T3-L1 adipocytes, co-culture with a macrophage cell line, RAW264, or TNFα remarkably enhanced GPR84 expression. In the presence of TNFα, MCFAs down-regulated adiponectin mRNA expression in 3T3-L1 adipocytes. Taken together, our results suggest that GPR84 emerges in adipocytes in response to TNFα from infiltrating macrophages and exacerbates the vicious cycle between adiposity and diabesity.

AB - In this study we aimed to identify the physiological roles of G protein-coupled receptor 84 (GPR84) in adipose tissue, together with medium-chain fatty acids (MCFAs), the specific ligands for GPR84. In mice, high-fat diet up-regulated GPR84 expression in fat pads. In 3T3-L1 adipocytes, co-culture with a macrophage cell line, RAW264, or TNFα remarkably enhanced GPR84 expression. In the presence of TNFα, MCFAs down-regulated adiponectin mRNA expression in 3T3-L1 adipocytes. Taken together, our results suggest that GPR84 emerges in adipocytes in response to TNFα from infiltrating macrophages and exacerbates the vicious cycle between adiposity and diabesity.

KW - Adiponectin

KW - Adipose tissue

KW - GPR84

KW - Medium-chain fatty acid

KW - TNFα

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DO - 10.1016/j.febslet.2012.01.001

M3 - Article

C2 - 22245676

AN - SCOPUS:84857051975

SN - 0014-5793

VL - 586

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EP - 372

JO - FEBS Letters

JF - FEBS Letters

IS - 4

ER -

Inflammatory changes in adipose tissue enhance expression of GPR84, a medium-chain fatty acid receptor: TNFα enhances GPR84 expression in adipocytes

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