Influence of humoral immunoreaction on hepatic nonparenchymal cells in ex situ xenoperfused rat livers

Takehiko Uesugi, Iwao Ikai, Seiji Satoh, Toshikazu Yagi, Akiyoshi Kanazawa, Osamu Takeyama, Ryuta Nishitai, Hiroshi Okabe, Nagato Katsura, Hiroaki Terajima, Rei Takahashi, Yoshio Yamaoka

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2 Citations (Scopus)

Abstract

Background. The influence of xenogeneic humoral immunoreaction on hepatic nonparenchymal cells (NPCs) was evaluated ex situ in xenoperfused rat livers. Methods. Isolated rat livers were perfused via the portal vein (PV) for 240 min. The perfusates consisted of fresh rat blood (group 1), fresh human blood (group 2), and fresh human blood containing 5 μg/mL soluble complement receptor type 1 (Group 3). Results. Deposition of human IgM and C5b-9 complement was observed in group 2, although only human IgM deposition was detected in group 3. Portal vein pressure in group 2 rose drastically during the first 10 min. Creatine kinase BB component gradually increased in all groups, followed by an elevation in alanine aminotransferase and both parameters were significantly higher in group 2 than in groups 1 and 3. In group 2, platelet thrombi in the peripheral PVs and periportal hemorrhage were observed after 10 min, and massive necrosis around the central veins after 240 rain; these changes were not observed in group 1 or 3. Production of tumor necrosis factor α and α interferon and expression of intercellular adhesion molecule 1 (ICAM-1) were lower in group 2 than in groups 1 and 3. In group 2, there were negative areas for ICAM-1 and tumor necrosis factor α staining around the central veins after 240 min, which were consistent with necrotic areas. Conclusions. In xenoperfused rat livers, humoral mediators initially caused the disturbance of microcirculation, which would induce long ischemia in the pericentral areas, resulting in massive necrosis. NPC necrosis may be responsible for less production of cytokines and adhesion molecules in the xenoperfused livers.

Original languageEnglish
Pages (from-to)272-281
Number of pages10
JournalJournal of Surgical Research
Volume99
Issue number2
DOIs
Publication statusPublished - 2001
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Surgery

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