Influence of IL17A polymorphisms (rs2275913 and rs3748067) on the susceptibility to ulcerative colitis

Ranji Hayashi, Tomomitsu Tahara, Hisakazu Shiroeda, Takashi Saito, Masakatsu Nakamura, Mikihiro Tsutsumi, Tomoyuki Shibata, Tomiyasu Arisawa

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Interleukin-17A plays a role in tissue inflammation by inducing release of proinflammatory and neutrophil-mobilizing cytokines. We investigated the association between ulcerative colitis (UC) and polymorphisms of IL17A, rs2275913 (-197 G > A), and rs3748067 (*1249 C > T). The study was performed in 475 healthy subjects (controls) and 202 with UC (UC cases), including 113 controls and 64 UC cases from previous study. We employed the multiplex PCR-SSCP method to detect gene polymorphisms. The minor allele frequency of rs2275913 was significantly higher but that of rs3748067 was significantly lower in UC cases than controls. The rs2275913 minor homozygote (AA) had an increased risk of the development of UC, whereas rs3748067 minor carrier (CT + TT) had decreased risks for the development of UC. When compared with LR group (rs2275913 GG + GA with rs3748067 CT + TT), HR group (rs2275913 AA with rs3748067 CC) had a more increased risk of the development of UC (OR, 3.38; p = 0.0007). The polymorphisms of IL17A were associated with the noncontinuous and pancolitis phenotypes of UC. Our results suggest that IL17A polymorphisms (both rs2275913 and rs3748067) influence the susceptibility to and pathophysiological features of UC, coordinately.

Original languageEnglish
Pages (from-to)239-244
Number of pages6
JournalClinical and Experimental Medicine
Volume13
Issue number4
DOIs
Publication statusPublished - 01-11-2013

Fingerprint

Polymorphism
Ulcerative Colitis
Interleukin-17
Genes
Tissue
Cytokines
Single-Stranded Conformational Polymorphism
Multiplex Polymerase Chain Reaction
Homozygote
Gene Frequency
Healthy Volunteers
Neutrophils
Inflammation
Phenotype

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Hayashi, Ranji ; Tahara, Tomomitsu ; Shiroeda, Hisakazu ; Saito, Takashi ; Nakamura, Masakatsu ; Tsutsumi, Mikihiro ; Shibata, Tomoyuki ; Arisawa, Tomiyasu. / Influence of IL17A polymorphisms (rs2275913 and rs3748067) on the susceptibility to ulcerative colitis. In: Clinical and Experimental Medicine. 2013 ; Vol. 13, No. 4. pp. 239-244.
@article{8ff3d59e04b24c9e9d6a1eba9781690c,
title = "Influence of IL17A polymorphisms (rs2275913 and rs3748067) on the susceptibility to ulcerative colitis",
abstract = "Interleukin-17A plays a role in tissue inflammation by inducing release of proinflammatory and neutrophil-mobilizing cytokines. We investigated the association between ulcerative colitis (UC) and polymorphisms of IL17A, rs2275913 (-197 G > A), and rs3748067 (*1249 C > T). The study was performed in 475 healthy subjects (controls) and 202 with UC (UC cases), including 113 controls and 64 UC cases from previous study. We employed the multiplex PCR-SSCP method to detect gene polymorphisms. The minor allele frequency of rs2275913 was significantly higher but that of rs3748067 was significantly lower in UC cases than controls. The rs2275913 minor homozygote (AA) had an increased risk of the development of UC, whereas rs3748067 minor carrier (CT + TT) had decreased risks for the development of UC. When compared with LR group (rs2275913 GG + GA with rs3748067 CT + TT), HR group (rs2275913 AA with rs3748067 CC) had a more increased risk of the development of UC (OR, 3.38; p = 0.0007). The polymorphisms of IL17A were associated with the noncontinuous and pancolitis phenotypes of UC. Our results suggest that IL17A polymorphisms (both rs2275913 and rs3748067) influence the susceptibility to and pathophysiological features of UC, coordinately.",
author = "Ranji Hayashi and Tomomitsu Tahara and Hisakazu Shiroeda and Takashi Saito and Masakatsu Nakamura and Mikihiro Tsutsumi and Tomoyuki Shibata and Tomiyasu Arisawa",
year = "2013",
month = "11",
day = "1",
doi = "10.1007/s10238-012-0206-5",
language = "English",
volume = "13",
pages = "239--244",
journal = "Clinical and Experimental Medicine",
issn = "1591-8890",
publisher = "Springer-Verlag Italia",
number = "4",

}

Hayashi, R, Tahara, T, Shiroeda, H, Saito, T, Nakamura, M, Tsutsumi, M, Shibata, T & Arisawa, T 2013, 'Influence of IL17A polymorphisms (rs2275913 and rs3748067) on the susceptibility to ulcerative colitis', Clinical and Experimental Medicine, vol. 13, no. 4, pp. 239-244. https://doi.org/10.1007/s10238-012-0206-5

Influence of IL17A polymorphisms (rs2275913 and rs3748067) on the susceptibility to ulcerative colitis. / Hayashi, Ranji; Tahara, Tomomitsu; Shiroeda, Hisakazu; Saito, Takashi; Nakamura, Masakatsu; Tsutsumi, Mikihiro; Shibata, Tomoyuki; Arisawa, Tomiyasu.

In: Clinical and Experimental Medicine, Vol. 13, No. 4, 01.11.2013, p. 239-244.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Influence of IL17A polymorphisms (rs2275913 and rs3748067) on the susceptibility to ulcerative colitis

AU - Hayashi, Ranji

AU - Tahara, Tomomitsu

AU - Shiroeda, Hisakazu

AU - Saito, Takashi

AU - Nakamura, Masakatsu

AU - Tsutsumi, Mikihiro

AU - Shibata, Tomoyuki

AU - Arisawa, Tomiyasu

PY - 2013/11/1

Y1 - 2013/11/1

N2 - Interleukin-17A plays a role in tissue inflammation by inducing release of proinflammatory and neutrophil-mobilizing cytokines. We investigated the association between ulcerative colitis (UC) and polymorphisms of IL17A, rs2275913 (-197 G > A), and rs3748067 (*1249 C > T). The study was performed in 475 healthy subjects (controls) and 202 with UC (UC cases), including 113 controls and 64 UC cases from previous study. We employed the multiplex PCR-SSCP method to detect gene polymorphisms. The minor allele frequency of rs2275913 was significantly higher but that of rs3748067 was significantly lower in UC cases than controls. The rs2275913 minor homozygote (AA) had an increased risk of the development of UC, whereas rs3748067 minor carrier (CT + TT) had decreased risks for the development of UC. When compared with LR group (rs2275913 GG + GA with rs3748067 CT + TT), HR group (rs2275913 AA with rs3748067 CC) had a more increased risk of the development of UC (OR, 3.38; p = 0.0007). The polymorphisms of IL17A were associated with the noncontinuous and pancolitis phenotypes of UC. Our results suggest that IL17A polymorphisms (both rs2275913 and rs3748067) influence the susceptibility to and pathophysiological features of UC, coordinately.

AB - Interleukin-17A plays a role in tissue inflammation by inducing release of proinflammatory and neutrophil-mobilizing cytokines. We investigated the association between ulcerative colitis (UC) and polymorphisms of IL17A, rs2275913 (-197 G > A), and rs3748067 (*1249 C > T). The study was performed in 475 healthy subjects (controls) and 202 with UC (UC cases), including 113 controls and 64 UC cases from previous study. We employed the multiplex PCR-SSCP method to detect gene polymorphisms. The minor allele frequency of rs2275913 was significantly higher but that of rs3748067 was significantly lower in UC cases than controls. The rs2275913 minor homozygote (AA) had an increased risk of the development of UC, whereas rs3748067 minor carrier (CT + TT) had decreased risks for the development of UC. When compared with LR group (rs2275913 GG + GA with rs3748067 CT + TT), HR group (rs2275913 AA with rs3748067 CC) had a more increased risk of the development of UC (OR, 3.38; p = 0.0007). The polymorphisms of IL17A were associated with the noncontinuous and pancolitis phenotypes of UC. Our results suggest that IL17A polymorphisms (both rs2275913 and rs3748067) influence the susceptibility to and pathophysiological features of UC, coordinately.

UR - http://www.scopus.com/inward/record.url?scp=84888378587&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84888378587&partnerID=8YFLogxK

U2 - 10.1007/s10238-012-0206-5

DO - 10.1007/s10238-012-0206-5

M3 - Article

C2 - 22955700

AN - SCOPUS:84888378587

VL - 13

SP - 239

EP - 244

JO - Clinical and Experimental Medicine

JF - Clinical and Experimental Medicine

SN - 1591-8890

IS - 4

ER -