Influence of sepsis in rats on muscle protein turnover in vivo and in tissue incubated under different in vitro conditions

Marianne Hall-Angerås, Ulf Angerås, Daniel von Allmen, Takashi Higashiguchi, Oded Zamir, Per Olof Hasselgren, Josef E. Fischer

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Abstract

We studied the influence of sepsis on muscle protein synthesis and degradation in vivo and in muscles, incubated flaccid or at resting length. Sepsis was induced in rats by cecal ligation and puncture (CLP). Control rats were sham-operated. A flooding dose of 14C-phenylalanine was used to determine muscle protein synthesis rate in vivo, and protein breakdown was calculated from the difference between protein synthesis and growth rates. Protein synthesis rate in vitro was assessed by determining incorporation of 14C-phenylalanine into protein in incubated extensor digitorum longus (EDL) and soleus (SOL) muscles. Total and myofibrillar protein breakdown rates were determined from release into incubation medium of tyrosine and 3-methylhistidine (3-MH), respectively. Muscle protein synthesis rate in vivo was reduced by 35%, similar to the reduction observed in muscles incubated flaccid or at resting length. The calculated protein breakdown rate in vivo was increased by 31% in septic rats. In incubated muscles, the increase in total protein breakdown (ie, tyrosine release) during sepsis was almost identical in muscles incubated flaccid or at resting length, ie, 83% to 88% in EDL and 47% to 49% in SOL. Myofibrillar protein degradation in vitro (ie, 3-MH release) was increased approximately 10-fold in EDL muscles incubated flaccid or at resting length, but was not significantly affected by sepsis in SOL. Results suggest that sepsis-induced changes in protein synthesis observed in muscles incubated either flaccid or at resting length reflect changes in vivo. Changes in protein breakdown were qualitatively similar in vivo and in vitro, but results in incubated muscles may overestimate the increase in muscle proteolysis caused by sepsis.

Original languageEnglish
Pages (from-to)247-251
Number of pages5
JournalMetabolism
Volume40
Issue number3
DOIs
Publication statusPublished - 01-01-1991
Externally publishedYes

Fingerprint

Muscle Proteins
Sepsis
Muscles
Proteins
Proteolysis
Phenylalanine
Tyrosine
In Vitro Techniques
Punctures
Ligation
Skeletal Muscle

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Cite this

Hall-Angerås, Marianne ; Angerås, Ulf ; von Allmen, Daniel ; Higashiguchi, Takashi ; Zamir, Oded ; Hasselgren, Per Olof ; Fischer, Josef E. / Influence of sepsis in rats on muscle protein turnover in vivo and in tissue incubated under different in vitro conditions. In: Metabolism. 1991 ; Vol. 40, No. 3. pp. 247-251.
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Influence of sepsis in rats on muscle protein turnover in vivo and in tissue incubated under different in vitro conditions. / Hall-Angerås, Marianne; Angerås, Ulf; von Allmen, Daniel; Higashiguchi, Takashi; Zamir, Oded; Hasselgren, Per Olof; Fischer, Josef E.

In: Metabolism, Vol. 40, No. 3, 01.01.1991, p. 247-251.

Research output: Contribution to journalArticle

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AU - Hall-Angerås, Marianne

AU - Angerås, Ulf

AU - von Allmen, Daniel

AU - Higashiguchi, Takashi

AU - Zamir, Oded

AU - Hasselgren, Per Olof

AU - Fischer, Josef E.

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