TY - JOUR
T1 - Infrequent frameshift mutations of polynucleotide repeats in multiple primary cancers affecting the esophagus and other organs
AU - Iwaya, Takeshi
AU - Maesawa, Chihaya
AU - Nishizuka, Satoshi
AU - Suzuki, Yasushi
AU - Sakata, Ken
AU - Sato, Nobuhiro
AU - Ikeda, Kenichiro
AU - Koeda, Keisuke
AU - Ogasawara, Satoshi
AU - Otsuka, Koki
AU - Kimura, Yusuke
AU - Aoki, Kiichi
AU - Ishida, Kaoru
AU - Saito, Kazuyoshi
AU - Tamura, Gen
PY - 1998/12
Y1 - 1998/12
N2 - Frequent frameshift mutations of simple nucleotide repeats in the protein-encoding regions, as well as replication errors (RERs) at microsatellite loci, have recently been demonstrated in gastrointestinal tumors. These genetic instabilities have been considered indicative of an increased risk of accumulating mutations in cancer-associated genes and of developing multiple cancers. We studied frameshift (or insertion/deletion) mutations of simple nucleotide repeats in five genes (TGFβ type II receptor [TGFβRII], E2F4, MSH2, MSH3, and MSH6) in 23 tumors from 12 patients who had synchronous cancers of the esophagus and other organs. Genetic instability at four microsatellite loci, as well as mutations in the TP53, APC, and KRAS2 genes, were also studied. No frameshift mutations were observed in the TGFβRII, MSH3, and MSH6 genes. RER and a deletion mutation of BAT26 in MSH2 were present in one (1/23; 4%) gastric cancer. This tumor also carried a deletion mutation in the serine (AGC) repeat of the E2F4 gene. Mutation screening of the TP53, APC, and KRAS2 genes revealed that the synchronous cancers did not carry the same mutations. Our results suggested that genetic instability, such as insertion/deletion mutations in simple nucleotide repeats, is not significantly associated with the development of multiple primary cancers of the esophagus and other organs, and that these synchronous cancers developed independently according to their different environmental factors.
AB - Frequent frameshift mutations of simple nucleotide repeats in the protein-encoding regions, as well as replication errors (RERs) at microsatellite loci, have recently been demonstrated in gastrointestinal tumors. These genetic instabilities have been considered indicative of an increased risk of accumulating mutations in cancer-associated genes and of developing multiple cancers. We studied frameshift (or insertion/deletion) mutations of simple nucleotide repeats in five genes (TGFβ type II receptor [TGFβRII], E2F4, MSH2, MSH3, and MSH6) in 23 tumors from 12 patients who had synchronous cancers of the esophagus and other organs. Genetic instability at four microsatellite loci, as well as mutations in the TP53, APC, and KRAS2 genes, were also studied. No frameshift mutations were observed in the TGFβRII, MSH3, and MSH6 genes. RER and a deletion mutation of BAT26 in MSH2 were present in one (1/23; 4%) gastric cancer. This tumor also carried a deletion mutation in the serine (AGC) repeat of the E2F4 gene. Mutation screening of the TP53, APC, and KRAS2 genes revealed that the synchronous cancers did not carry the same mutations. Our results suggested that genetic instability, such as insertion/deletion mutations in simple nucleotide repeats, is not significantly associated with the development of multiple primary cancers of the esophagus and other organs, and that these synchronous cancers developed independently according to their different environmental factors.
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U2 - 10.1002/(SICI)1098-2264(199812)23:4<317::AID-GCC6>3.0.CO;2-W
DO - 10.1002/(SICI)1098-2264(199812)23:4<317::AID-GCC6>3.0.CO;2-W
M3 - Article
C2 - 9824204
AN - SCOPUS:0031767913
SN - 1045-2257
VL - 23
SP - 317
EP - 322
JO - Genes Chromosomes and Cancer
JF - Genes Chromosomes and Cancer
IS - 4
ER -