Inhibition by the cold receptor agonists menthol and icilin of airway smooth muscle contraction

Satoru Ito, Hiroaki Kume, Akira Shiraki, Masashi Kondo, Yasushi Makino, Kaichiro Kamiya, Yoshinori Hasegawa

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Menthol, known as a cold receptor agonist, has widely been used in the relief of respiratory symptoms such as coughing and chest congestion. Previous studies have demonstrated that menthol reduces bronchoconstriction and airway hyperresponsiveness. The aim of this study was to examine the effects of menthol and icilin, another cold receptor agonist, on airway smooth muscle contraction. Isometric force was monitored using epithelium-denuded tracheal smooth muscle tissues isolated from guinea pigs. Intracellular Ca2+ concentrations were assessed by fura-2 fluorescence. (-)Menthol (0.01-1 mM) inhibited contraction induced by methacholine (MCh, 0.01-10 μM) and high extracellular K+ concentrations (20-60 mM) in a concentration-dependent manner. Moreover, the increases of intracellular Ca2+ concentrations induced by MCh or high K+ were significantly reduced by (-)menthol. Icilin (100 μM) also significantly attenuated contraction induced by MCh or high K+. The inhibitory effect of 1 mM (-)menthol on MCh-induced contraction was significantly higher at cool temperature (24-26 °C) than at 37 °C. The present results demonstrate that inhibition of Ca2+ influx plays an important role in the menthol-mediated inhibition of contraction in airway smooth muscle. Furthermore, our findings indicate that stimulation of unknown cold receptors may be involved in these mechanisms. These findings suggest that the use of menthol is beneficial for reducing respiratory symptoms because of its inhibitory effects on airway smooth muscle contraction.

Original languageEnglish
Pages (from-to)812-817
Number of pages6
JournalPulmonary Pharmacology and Therapeutics
Volume21
Issue number5
DOIs
Publication statusPublished - 01-10-2008

Fingerprint

Menthol
Muscle Contraction
Smooth Muscle
Muscle
Bronchoconstriction
Methacholine Chloride
icilin
Guinea Pigs
Thorax
Epithelium
Fluorescence
Tissue
Muscles
Temperature

All Science Journal Classification (ASJC) codes

  • Pulmonary and Respiratory Medicine
  • Biochemistry, medical
  • Pharmacology (medical)

Cite this

Ito, Satoru ; Kume, Hiroaki ; Shiraki, Akira ; Kondo, Masashi ; Makino, Yasushi ; Kamiya, Kaichiro ; Hasegawa, Yoshinori. / Inhibition by the cold receptor agonists menthol and icilin of airway smooth muscle contraction. In: Pulmonary Pharmacology and Therapeutics. 2008 ; Vol. 21, No. 5. pp. 812-817.
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Inhibition by the cold receptor agonists menthol and icilin of airway smooth muscle contraction. / Ito, Satoru; Kume, Hiroaki; Shiraki, Akira; Kondo, Masashi; Makino, Yasushi; Kamiya, Kaichiro; Hasegawa, Yoshinori.

In: Pulmonary Pharmacology and Therapeutics, Vol. 21, No. 5, 01.10.2008, p. 812-817.

Research output: Contribution to journalArticle

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