Inhibition of azoxymethane-induced aberrant crypt foci formation in rat colorectum by whole leaf Aloe arborescens Miller var. natalensis Berger

Kan Shimpo, Takeshi Chihara, Hidehiko Beppu, Chikako Ida, Takaaki Kaneko, Toshiharu Nagatsu, Hiroshi Kuzuya

Research output: Contribution to journalArticle

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Abstract

We examined the modifying effect of whole-leaf Aloe arborescens Miller var. natalensis Berger (designated as 'ALOE') on azoxymethane (AOM)-induced aberrant crypt foci (ACF), putative preneoplastic lesions, in the rat colorectum. Male F344 rats (4 weeks old) were fed the basal diet, or experimental diets containing 1% or 5% ALOE for 5 weeks. One week later, all rats except those in the vehicle-treated groups were injected s.c. with AOM (15 mg/kg, once weekly for 3 weeks). At 9 weeks of age, all the rats were killed, and the colorectum and liver were evaluated for ACF and cytosolic quinone reductase (QR; a phase 2 enzyme), respectively. In rats given AOM and ALOE (1% or 5% in diet) the numbers of ACF/colorectum, aberrant crypts/colorectum, aberrant crypts/focus and large ACF/colorectum were significantly decreased compared with those of rats given AOM alone (all p < 0.01). No ACF were found in rats treated without AOM. In addition, ALOE significantly increased cytosolic QR activity in the liver (p < 0.01). These results indicated that ALOE inhibited the development of AOM-induced ACF in the rat colorectum, with increased QR activity in the liver, and therefore suggested that ALOE might have a chemopreventive effect against colon carcinogenesis at least in the initiation stage.

Original languageEnglish
Pages (from-to)705-711
Number of pages7
JournalPhytotherapy Research
Volume15
Issue number8
DOIs
Publication statusPublished - 01-12-2001

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Aberrant Crypt Foci
Azoxymethane
Aloe
Diet
Liver
NAD(P)H Dehydrogenase (Quinone)
Inbred F344 Rats
Colon
Carcinogenesis
Enzymes

All Science Journal Classification (ASJC) codes

  • Pharmacology

Cite this

Shimpo, Kan ; Chihara, Takeshi ; Beppu, Hidehiko ; Ida, Chikako ; Kaneko, Takaaki ; Nagatsu, Toshiharu ; Kuzuya, Hiroshi. / Inhibition of azoxymethane-induced aberrant crypt foci formation in rat colorectum by whole leaf Aloe arborescens Miller var. natalensis Berger. In: Phytotherapy Research. 2001 ; Vol. 15, No. 8. pp. 705-711.
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abstract = "We examined the modifying effect of whole-leaf Aloe arborescens Miller var. natalensis Berger (designated as 'ALOE') on azoxymethane (AOM)-induced aberrant crypt foci (ACF), putative preneoplastic lesions, in the rat colorectum. Male F344 rats (4 weeks old) were fed the basal diet, or experimental diets containing 1{\%} or 5{\%} ALOE for 5 weeks. One week later, all rats except those in the vehicle-treated groups were injected s.c. with AOM (15 mg/kg, once weekly for 3 weeks). At 9 weeks of age, all the rats were killed, and the colorectum and liver were evaluated for ACF and cytosolic quinone reductase (QR; a phase 2 enzyme), respectively. In rats given AOM and ALOE (1{\%} or 5{\%} in diet) the numbers of ACF/colorectum, aberrant crypts/colorectum, aberrant crypts/focus and large ACF/colorectum were significantly decreased compared with those of rats given AOM alone (all p < 0.01). No ACF were found in rats treated without AOM. In addition, ALOE significantly increased cytosolic QR activity in the liver (p < 0.01). These results indicated that ALOE inhibited the development of AOM-induced ACF in the rat colorectum, with increased QR activity in the liver, and therefore suggested that ALOE might have a chemopreventive effect against colon carcinogenesis at least in the initiation stage.",
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Inhibition of azoxymethane-induced aberrant crypt foci formation in rat colorectum by whole leaf Aloe arborescens Miller var. natalensis Berger. / Shimpo, Kan; Chihara, Takeshi; Beppu, Hidehiko; Ida, Chikako; Kaneko, Takaaki; Nagatsu, Toshiharu; Kuzuya, Hiroshi.

In: Phytotherapy Research, Vol. 15, No. 8, 01.12.2001, p. 705-711.

Research output: Contribution to journalArticle

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T1 - Inhibition of azoxymethane-induced aberrant crypt foci formation in rat colorectum by whole leaf Aloe arborescens Miller var. natalensis Berger

AU - Shimpo, Kan

AU - Chihara, Takeshi

AU - Beppu, Hidehiko

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AU - Kaneko, Takaaki

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AU - Kuzuya, Hiroshi

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