Inhibition of azoxymethane-induced colorectal aberrant crypt foci in mice fed a high-fat diet by Pleurotus eryngii (Eringi) and hypsizygus marmoreus (Bunashimeji)

Takaaki Kaneko, Takeshi Chihara, Kan Shimpo, Hidehiko Beppu, Takashi Higashiguchi, Shigeru Sonoda

Research output: Contribution to journalArticle

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Abstract

Obesity markedly increases the risk of colorectal cancer. Recently, the preventive effects of edible mushrooms on triglyceride elevation and visceral fat accumulation have been reported. Here, the effects of Pleurotus eryngii (Eringi) and Hypsizygus marmoreus (Bunashimeji) on azoxymethane (AOM)-induced aberrant crypt foci (ACF; precancerous lesions) in the colorectums of mice fed a high-fat diet were examined. Eringi (ER) and Bunashimeji (BU) mushroom powder samples were used. Six-week-old male C57BL/6J mice received an intraperitoneal injection of AOM (10 mg/kg) once a week for two weeks, and were sacrificed and dissected at 6 weeks after the start of the experiment. After the initiation of the experiment, they received a normal diet (ND), high-fat diet (HFD), HFD + ER (1 or 5% of diet), or HFD + BU (1 or 5% of diet). As a result, body and fat weights were significantly lower in the 5% ER and BU groups than in the HFD group. Liver triglyceride levels were also significantly lower in the 5% ER and BU groups. Total liver cholesterol levels were significantly lower in the 5% ER group. The numbers of ACF (especially large ACF) showed strong inhibitory effects in both ER and BU groups. Measurement of the cell proliferation marker Ki-67 labeling index in the colonic mucosa demonstrated more significant suppression in both ER and BU groups than in the HFD group. These results suggest that the simultaneous intake of ER and BU may inhibit colorectal tumorigenesis in HFD-fed mice.

Original languageEnglish
Pages (from-to)3881-3885
Number of pages5
JournalAsian Pacific Journal of Cancer Prevention
Volume16
Issue number9
DOIs
Publication statusPublished - 01-01-2015

Fingerprint

Aberrant Crypt Foci
Azoxymethane
Pleurotus
High Fat Diet
Agaricales
Diet
Triglycerides
Intra-Abdominal Fat
Liver
Intraperitoneal Injections
Inbred C57BL Mouse
Powders
Adipose Tissue
Colorectal Neoplasms
Carcinogenesis
Mucous Membrane
Obesity
Cholesterol
Body Weight
Cell Proliferation

All Science Journal Classification (ASJC) codes

  • Epidemiology
  • Oncology
  • Public Health, Environmental and Occupational Health
  • Cancer Research

Cite this

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title = "Inhibition of azoxymethane-induced colorectal aberrant crypt foci in mice fed a high-fat diet by Pleurotus eryngii (Eringi) and hypsizygus marmoreus (Bunashimeji)",
abstract = "Obesity markedly increases the risk of colorectal cancer. Recently, the preventive effects of edible mushrooms on triglyceride elevation and visceral fat accumulation have been reported. Here, the effects of Pleurotus eryngii (Eringi) and Hypsizygus marmoreus (Bunashimeji) on azoxymethane (AOM)-induced aberrant crypt foci (ACF; precancerous lesions) in the colorectums of mice fed a high-fat diet were examined. Eringi (ER) and Bunashimeji (BU) mushroom powder samples were used. Six-week-old male C57BL/6J mice received an intraperitoneal injection of AOM (10 mg/kg) once a week for two weeks, and were sacrificed and dissected at 6 weeks after the start of the experiment. After the initiation of the experiment, they received a normal diet (ND), high-fat diet (HFD), HFD + ER (1 or 5{\%} of diet), or HFD + BU (1 or 5{\%} of diet). As a result, body and fat weights were significantly lower in the 5{\%} ER and BU groups than in the HFD group. Liver triglyceride levels were also significantly lower in the 5{\%} ER and BU groups. Total liver cholesterol levels were significantly lower in the 5{\%} ER group. The numbers of ACF (especially large ACF) showed strong inhibitory effects in both ER and BU groups. Measurement of the cell proliferation marker Ki-67 labeling index in the colonic mucosa demonstrated more significant suppression in both ER and BU groups than in the HFD group. These results suggest that the simultaneous intake of ER and BU may inhibit colorectal tumorigenesis in HFD-fed mice.",
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Inhibition of azoxymethane-induced colorectal aberrant crypt foci in mice fed a high-fat diet by Pleurotus eryngii (Eringi) and hypsizygus marmoreus (Bunashimeji). / Kaneko, Takaaki; Chihara, Takeshi; Shimpo, Kan; Beppu, Hidehiko; Higashiguchi, Takashi; Sonoda, Shigeru.

In: Asian Pacific Journal of Cancer Prevention, Vol. 16, No. 9, 01.01.2015, p. 3881-3885.

Research output: Contribution to journalArticle

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AU - Kaneko, Takaaki

AU - Chihara, Takeshi

AU - Shimpo, Kan

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AU - Higashiguchi, Takashi

AU - Sonoda, Shigeru

PY - 2015/1/1

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