Inhibition of ENNG-induced pyloric stomach and small intestinal carcinogenesis in mice by high temperature- and pressure-treated garlic

Takaaki Kaneko, Kan Shimpo, Takeshi Chihara, Hidehiko Beppu, Akiko Tomatsu, Masanori Shinzato, Takamasa Yanagida, Tsutomu Ieike, Shigeru Sonoda, Akihiko Futamura, Akihiro Ito, Takashi Higashiguchi

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Abstract

High temperature- and pressure-treated garlic (HTPG) has been shown to have enhanced antioxidative activity and polyphenol contents. Previously, we reported that HTPG inhibited 1,2-dimethylhydrazine-induced mucin depleted foci (premalignant lesions) and O6-methylguanine DNA adduct formation in the rat colorectum. In the present study, we investigated the modifying effects of HTPG on N-ethyl-N'-nitro-N-nitrosoguanidine (ENNG)-induced pyloric stomach and small intestinal carcinogenesis in mice. Male C57BL/6 mice were given ENNG (100 mg/l) in drinking water for the first 4 weeks, then a basal diet or diet containing 2% or 5% HTPG for 30 weeks. The incidence and multiplicity of pyloric stomach and small intestinal (duodenal and jejunal) tumors in the 2% HTPG group (but not in the 5% HTPG group) were significantly lower than those in the control group. Cell proliferation of normal-appearing duodenal mucosa was assessed by MIB-5 immunohistochemistry and shown to be significantly lower with 2% HTPG (but again not 5% HTPG) than in controls. These results in dicate that HTPG, at 2% in the diet, inhibited ENNG-induced pyloric stomach and small intestinal (especially duodenal) tumorigenesis in mice, associated with suppression of cell proliferation.

Original languageEnglish
Pages (from-to)1983-1988
Number of pages6
JournalAsian Pacific Journal of Cancer Prevention
Volume13
Issue number5
DOIs
Publication statusPublished - 01-01-2012

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Garlic
Stomach
Carcinogenesis
Pressure
Temperature
Diet
Cell Proliferation
1,2-Dimethylhydrazine
ENNG
DNA Adducts
Polyphenols
Mucins
Inbred C57BL Mouse
Drinking Water
Mucous Membrane
Immunohistochemistry
Control Groups
Incidence

All Science Journal Classification (ASJC) codes

  • Epidemiology
  • Oncology
  • Public Health, Environmental and Occupational Health
  • Cancer Research

Cite this

Kaneko, Takaaki ; Shimpo, Kan ; Chihara, Takeshi ; Beppu, Hidehiko ; Tomatsu, Akiko ; Shinzato, Masanori ; Yanagida, Takamasa ; Ieike, Tsutomu ; Sonoda, Shigeru ; Futamura, Akihiko ; Ito, Akihiro ; Higashiguchi, Takashi. / Inhibition of ENNG-induced pyloric stomach and small intestinal carcinogenesis in mice by high temperature- and pressure-treated garlic. In: Asian Pacific Journal of Cancer Prevention. 2012 ; Vol. 13, No. 5. pp. 1983-1988.
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abstract = "High temperature- and pressure-treated garlic (HTPG) has been shown to have enhanced antioxidative activity and polyphenol contents. Previously, we reported that HTPG inhibited 1,2-dimethylhydrazine-induced mucin depleted foci (premalignant lesions) and O6-methylguanine DNA adduct formation in the rat colorectum. In the present study, we investigated the modifying effects of HTPG on N-ethyl-N'-nitro-N-nitrosoguanidine (ENNG)-induced pyloric stomach and small intestinal carcinogenesis in mice. Male C57BL/6 mice were given ENNG (100 mg/l) in drinking water for the first 4 weeks, then a basal diet or diet containing 2{\%} or 5{\%} HTPG for 30 weeks. The incidence and multiplicity of pyloric stomach and small intestinal (duodenal and jejunal) tumors in the 2{\%} HTPG group (but not in the 5{\%} HTPG group) were significantly lower than those in the control group. Cell proliferation of normal-appearing duodenal mucosa was assessed by MIB-5 immunohistochemistry and shown to be significantly lower with 2{\%} HTPG (but again not 5{\%} HTPG) than in controls. These results in dicate that HTPG, at 2{\%} in the diet, inhibited ENNG-induced pyloric stomach and small intestinal (especially duodenal) tumorigenesis in mice, associated with suppression of cell proliferation.",
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Inhibition of ENNG-induced pyloric stomach and small intestinal carcinogenesis in mice by high temperature- and pressure-treated garlic. / Kaneko, Takaaki; Shimpo, Kan; Chihara, Takeshi; Beppu, Hidehiko; Tomatsu, Akiko; Shinzato, Masanori; Yanagida, Takamasa; Ieike, Tsutomu; Sonoda, Shigeru; Futamura, Akihiko; Ito, Akihiro; Higashiguchi, Takashi.

In: Asian Pacific Journal of Cancer Prevention, Vol. 13, No. 5, 01.01.2012, p. 1983-1988.

Research output: Contribution to journalArticle

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AU - Kaneko, Takaaki

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AU - Tomatsu, Akiko

AU - Shinzato, Masanori

AU - Yanagida, Takamasa

AU - Ieike, Tsutomu

AU - Sonoda, Shigeru

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