Inhibition of ret tyrosine kinase activity by herbimycin A

Masahiko Taniguchi, Yoshimasa Uehara, Mutsushi Matsuyama, Masahide Takahashi

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

We examined the effect of herbimycin A, a potent inhibitor of tyrosine kinase, on NIH(ret) cells and TPC-1 papillary thyroid carcinoma cells, both of which express the active ret genes. Herbimycin A reversed the morphology of NIH(ret) cells to flat cells with a concomitant reassembly of microfilament bundles. On the other hand, it did not induce a significant change in cell shape of TPC-1 cells. When tyrosine kinase activities of the active ret gene products in herbimycin A-treated NIH(ret) and TPC-1 cells were examined in immunocomplex kinase assays, they drastically decreased in both cells as compared with untreated cells. In addition, herbimycin A strongly inhibited tyrosine phosphorylation of 40 kDa and 31 kDa proteins present in the immunoprecipitates of both cells, suggesting that these proteins could associate with the Ret proteins.

Original languageEnglish
Pages (from-to)208-214
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume195
Issue number1
DOIs
Publication statusPublished - 31-08-1993
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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