Inhibition of the activation of heat shock factor in vivo and in vitro by flavonoids

Nobuko Hosokawa, Kazunori Hirayoshi, Hitoshi Kudo, Hajime Takechi, Akira Aoike, Keiichi Kawai, Kazuhiro Nagata

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Abstract

Transcriptional activation of human heat shock protein (HSP) genes by heat shock or other stresses is regulated by the activation of a heat shock factor (HSF). Activated HSF posttranslationally acquires DNA-binding ability. We previously reported that quercetin and some other flavonoids inhibited the induction of HSPs in HeLa and COLO 320DM cells, derived from a human colon cancer, at the level of mRNA accumulation. In this study, we examined the effects of quercetin on the induction of HSP70 promoter-regulated chloramphenicol acetyltransferase (CAT) activity and on the binding of HSF to the heat shock element (HSE) by a gel mobility shift assay with extracts of COLO 320DM cells. Quercetin inhibited heat-induced CAT activity in COS-7 and COLO 320DM cells which were transfected with plasmids bearing the CAT gene under the control of the promoter region of the human HSP70 gene. Treatment with quercetin inhibited the binding of HSF to the HSE in whole-cell extracts activated in vivo by heat shock and in cytoplasmic extracts activated in vitro by elevated temperature or by urea. The binding of HSF activated in vitro by Nonidet P-40 was not suppressed by the addition of quercetin. The formation of the HSF-HSE complex was not inhibited when quercetin was added only during the binding reaction of HSF to the HSE after in vitro heat activation. Quercetin thus interacts with HSF and inhibits the induction of HSPs after heat shock through inhibition of HSF activation.

Original languageEnglish
Pages (from-to)3490-3498
Number of pages9
JournalMolecular and Cellular Biology
Volume12
Issue number8
DOIs
Publication statusPublished - 01-01-1992

Fingerprint

Flavonoids
Shock
Hot Temperature
Quercetin
Chloramphenicol O-Acetyltransferase
In Vitro Techniques
Genes
Heat-Shock Response
Electrophoretic Mobility Shift Assay
Heat-Shock Proteins
Cell Extracts
Genetic Promoter Regions
Colonic Neoplasms
Transcriptional Activation
Urea

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

Cite this

Hosokawa, Nobuko ; Hirayoshi, Kazunori ; Kudo, Hitoshi ; Takechi, Hajime ; Aoike, Akira ; Kawai, Keiichi ; Nagata, Kazuhiro. / Inhibition of the activation of heat shock factor in vivo and in vitro by flavonoids. In: Molecular and Cellular Biology. 1992 ; Vol. 12, No. 8. pp. 3490-3498.
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Hosokawa, N, Hirayoshi, K, Kudo, H, Takechi, H, Aoike, A, Kawai, K & Nagata, K 1992, 'Inhibition of the activation of heat shock factor in vivo and in vitro by flavonoids', Molecular and Cellular Biology, vol. 12, no. 8, pp. 3490-3498. https://doi.org/10.1128/MCB.12.8.3490

Inhibition of the activation of heat shock factor in vivo and in vitro by flavonoids. / Hosokawa, Nobuko; Hirayoshi, Kazunori; Kudo, Hitoshi; Takechi, Hajime; Aoike, Akira; Kawai, Keiichi; Nagata, Kazuhiro.

In: Molecular and Cellular Biology, Vol. 12, No. 8, 01.01.1992, p. 3490-3498.

Research output: Contribution to journalArticle

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AU - Hosokawa, Nobuko

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