Inhibition of the DNA-binding activity of NF-κB by gold compounds in vitro

Jian Ping Yang; Jocelyn P. Merin; Tatsunori Nakano; Tetsuji Kato; Yukio Kitade; Takashi Okamoto

doi:10.1016/0014-5793(95)00157-5

Inhibition of the DNA-binding activity of NF-κB by gold compounds in vitro

Jian Ping Yang, Jocelyn P. Merin, Tatsunori Nakano, Tetsuji Kato, Yukio Kitade, Takashi Okamoto

Research output: Contribution to journal › Article › peer-review

159 Citations (Scopus)

Abstract

Nuclear factor κB (NF-κB) is a transcription factor that is critical for the inducible expression of multiple cellular and viral genes. DNA binding activity is essential for its function. Here, we report that gold compounds, especially aurothioglucose (AuTG), have a strong inhibitory effect on NF-κB-DNA binding. Our finding also reveals that Zn²⁺ is a necessary component of NF-κB for its DNA binding activity and that gold ion can efficiently block NF-κB-DNA binding, presumably through oxidation of the cysteins associated with zinc. This redox mechanism may provide an explanation for the observed efficacy of gold compounds in the treatment of rheumatoid arthritis.

Original language	English
Pages (from-to)	89-96
Number of pages	8
Journal	FEBS Letters
Volume	361
Issue number	1
DOIs	https://doi.org/10.1016/0014-5793(95)00157-5
Publication status	Published - 13-03-1995
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Access to Document

10.1016/0014-5793(95)00157-5

Cite this

@article{94edd386e7bf4a6da25a9168629abeff,

title = "Inhibition of the DNA-binding activity of NF-κB by gold compounds in vitro",

abstract = "Nuclear factor κB (NF-κB) is a transcription factor that is critical for the inducible expression of multiple cellular and viral genes. DNA binding activity is essential for its function. Here, we report that gold compounds, especially aurothioglucose (AuTG), have a strong inhibitory effect on NF-κB-DNA binding. Our finding also reveals that Zn2+ is a necessary component of NF-κB for its DNA binding activity and that gold ion can efficiently block NF-κB-DNA binding, presumably through oxidation of the cysteins associated with zinc. This redox mechanism may provide an explanation for the observed efficacy of gold compounds in the treatment of rheumatoid arthritis.",

author = "Yang, {Jian Ping} and Merin, {Jocelyn P.} and Tatsunori Nakano and Tetsuji Kato and Yukio Kitade and Takashi Okamoto",

note = "Funding Information: Acknowledgements: We thank Drs. Shinsaku Sakurada and Hirotoshi Tanaka for helpful comments, and Dr. Librado A, Santiago for critical reading of this manuscript. J.-EY. is the recipient of a Sasagawa Medical fellowship. This work was supported by grants-in-aid from the Ministry of Health and Welfare and the Ministry of Education, Science and Culture of Japan.",

year = "1995",

month = mar,

day = "13",

doi = "10.1016/0014-5793(95)00157-5",

language = "English",

volume = "361",

pages = "89--96",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Elsevier",

number = "1",

}

TY - JOUR

T1 - Inhibition of the DNA-binding activity of NF-κB by gold compounds in vitro

AU - Yang, Jian Ping

AU - Merin, Jocelyn P.

AU - Nakano, Tatsunori

AU - Kato, Tetsuji

AU - Kitade, Yukio

AU - Okamoto, Takashi

N1 - Funding Information: Acknowledgements: We thank Drs. Shinsaku Sakurada and Hirotoshi Tanaka for helpful comments, and Dr. Librado A, Santiago for critical reading of this manuscript. J.-EY. is the recipient of a Sasagawa Medical fellowship. This work was supported by grants-in-aid from the Ministry of Health and Welfare and the Ministry of Education, Science and Culture of Japan.

PY - 1995/3/13

Y1 - 1995/3/13

N2 - Nuclear factor κB (NF-κB) is a transcription factor that is critical for the inducible expression of multiple cellular and viral genes. DNA binding activity is essential for its function. Here, we report that gold compounds, especially aurothioglucose (AuTG), have a strong inhibitory effect on NF-κB-DNA binding. Our finding also reveals that Zn2+ is a necessary component of NF-κB for its DNA binding activity and that gold ion can efficiently block NF-κB-DNA binding, presumably through oxidation of the cysteins associated with zinc. This redox mechanism may provide an explanation for the observed efficacy of gold compounds in the treatment of rheumatoid arthritis.

AB - Nuclear factor κB (NF-κB) is a transcription factor that is critical for the inducible expression of multiple cellular and viral genes. DNA binding activity is essential for its function. Here, we report that gold compounds, especially aurothioglucose (AuTG), have a strong inhibitory effect on NF-κB-DNA binding. Our finding also reveals that Zn2+ is a necessary component of NF-κB for its DNA binding activity and that gold ion can efficiently block NF-κB-DNA binding, presumably through oxidation of the cysteins associated with zinc. This redox mechanism may provide an explanation for the observed efficacy of gold compounds in the treatment of rheumatoid arthritis.

UR - http://www.scopus.com/inward/record.url?scp=0028903290&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028903290&partnerID=8YFLogxK

U2 - 10.1016/0014-5793(95)00157-5

DO - 10.1016/0014-5793(95)00157-5

M3 - Article

C2 - 7890047

AN - SCOPUS:0028903290

SN - 0014-5793

VL - 361

SP - 89

EP - 96

JO - FEBS Letters

JF - FEBS Letters

IS - 1

ER -

Inhibition of the DNA-binding activity of NF-κB by gold compounds in vitro

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this