Inhibition of the metallo-β-lactamase produced from Serratia marcescens by thiol compounds

Masafumi Goto; Tatsuya Takahashi; Fumiko Yamashita; Ako Koreeda; Hiromasa Mori; Michio Ohta; Yoshichika Arakawa

doi:10.1248/bpb.20.1136

Inhibition of the metallo-β-lactamase produced from Serratia marcescens by thiol compounds

Masafumi Goto, Tatsuya Takahashi, Fumiko Yamashita, Ako Koreeda, Hiromasa Mori, Michio Ohta, Yoshichika Arakawa

Research output: Contribution to journal › Article › peer-review

67 Citations (Scopus)

Abstract

Low molecular weight thiol compounds have been found to be strong inhibitors of metallo-β-lactamase (IMP-1) produced by Serratia marcescens TN9106, which was expressed by Echerichia coli JM109 cells. Mercaptoacetic acid and 2-mercaptopropionic acid strongly and competitively inhibited IMP-1 with K(i) of 0.23 and 0.19 μM, respectively. 2-Mercaptoethanol reversibly inhibited IMP-1 but did not show simple competitive inhibition.

Original language	English
Pages (from-to)	1136-1140
Number of pages	5
Journal	Biological and Pharmaceutical Bulletin
Volume	20
Issue number	11
DOIs	https://doi.org/10.1248/bpb.20.1136
Publication status	Published - 1997
Externally published	Yes

All Science Journal Classification (ASJC) codes

Pharmacology
Pharmaceutical Science

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10.1248/bpb.20.1136

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abstract = "Low molecular weight thiol compounds have been found to be strong inhibitors of metallo-β-lactamase (IMP-1) produced by Serratia marcescens TN9106, which was expressed by Echerichia coli JM109 cells. Mercaptoacetic acid and 2-mercaptopropionic acid strongly and competitively inhibited IMP-1 with K(i) of 0.23 and 0.19 μM, respectively. 2-Mercaptoethanol reversibly inhibited IMP-1 but did not show simple competitive inhibition.",

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AU - Goto, Masafumi

AU - Takahashi, Tatsuya

AU - Yamashita, Fumiko

AU - Koreeda, Ako

AU - Mori, Hiromasa

AU - Ohta, Michio

AU - Arakawa, Yoshichika

PY - 1997

Y1 - 1997

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AB - Low molecular weight thiol compounds have been found to be strong inhibitors of metallo-β-lactamase (IMP-1) produced by Serratia marcescens TN9106, which was expressed by Echerichia coli JM109 cells. Mercaptoacetic acid and 2-mercaptopropionic acid strongly and competitively inhibited IMP-1 with K(i) of 0.23 and 0.19 μM, respectively. 2-Mercaptoethanol reversibly inhibited IMP-1 but did not show simple competitive inhibition.

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Inhibition of the metallo-β-lactamase produced from Serratia marcescens by thiol compounds

Abstract

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