Inhibitory action of guanosine 3′,5′-monophosphate on thrombin-induced phosphatidylinositol turnover and protein phosphorylation in human platelets

Yoshimi Takai, Kozo Kaibuchi, Tsukasa Matsubara, Yasutomi Nishizuka

Research output: Contribution to journalArticle

96 Citations (Scopus)

Abstract

Human platelets activated by thrombin rapidly produce diglyceride from phosphatidylinositol (PI). Concomitantly, a protein having a molecular weight of about 40,000 is heavily phosphorylated and serotonin is released. These reactions are inhibited in parallel manners by sodium nitroprusside which elevates cyclic GMP. 8-Bromocyclic GMP also inhibits the reactions. Instead, both agents stimulate the phosphorylation of a distinct protein having a molecular weight of about 50,000. Since stimulants of platelets such as thrombin normally increase cyclic GMP, the results imply that this cyclic nucleotide may be involved in a circuit leading to the feedback inhibition of PI hydrolysis presumably through protein phosphorylation.

Original languageEnglish
Pages (from-to)61-67
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume101
Issue number1
DOIs
Publication statusPublished - 16-07-1981
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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