Inhibitory effects of freeze‐dried Aloe arborescens Miller var. natalensis Berger (Kidachi aloe) whole leaf powder (Aloe) on induction of preneoplastic glutathione S‐transferase (GST‐P) positive hepatocyte foci GST‐P+ were studied in male rats. Experiment 1: Group 1 was fed a diet containing 30% Aloe for 8 days. At day 7, the carcinogen, 2‐amino‐3‐methylimidazo(4,5‐f)quinoline(1Q, 100 mg/kg, i.g., one dose), was given 12 h after partial hepatectomy (PH) for initiation, followed by phenobarbital (PB) and D‐galactosamine for promotion and then killed at week 11. Experiment 2: Group 1 was given diethylnitrosamine (DEN, 200 mg/kg, i.p.) for initiation then a diet containing 30, 20 or 10% Aloe during weeks 3–8, with PH at week 3. In both experiments, groups 2 and 3 were treated as in group 1 without Aloe or carcinogens. GST‐P+ in group 1 lesions larger than 5 cells in experiment 1 and 200 μm in diameter in experiment 2 were compared with group 2. In experiment 1, the number of cells in group 1 showed a tendency for decrease. In experiment 2, the areas in group 1 were significantly decreased. The results indicate that 30% Aloe exerts inhibition of the promotion, and possibly of the initiation stage of hepatocarcinogenesis.
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