Initial characterization of the Epstein–Barr virus BSRF1 gene product

Yusuke Yanagi, H. M. Abdullah Al Masud, Takahiro Watanabe, Yoshitaka Sato, Fumi Goshima, Hiroshi Kimura, Takayuki Murata

Research output: Contribution to journalArticle

Abstract

Epstein–Barr virus (EBV) is a ubiquitous virus that causes infectious mononucleosis and several types of cancer, such as Burkitt lymphoma, T/NK-cell lymphoma, and nasopharyngeal carcinoma. As a herpesvirus, it encodes more than 80 genes, many of which have not been characterized. EBV BamHI S rightward reading frame 1 (BSRF1) encodes a tegument protein that, unlike its homologs herpes simplex virus unique long 51 (UL51) and human cytomegalovirus UL71, has not been extensively investigated. To examine the role of BSRF1, we prepared knockout and revertant strains using the bacterial artificial chromosome system. Unexpectedly, the disruption of the gene had little or no effect on EBV lytic replication and the transformation of primary B cells. However, the knockdown of BSRF1 in B95-8 cells decreased progeny production. An immunofluorescence assay revealed that BSRF1 localized to the Golgi apparatus in the cytoplasm, as did its homologs. BSRF1 also associated with BamHI G leftward reading frame 3.5 (BGLF3.5), BamHI B rightward reading frame 2 (BBRF2), and BamHI A leftward reading frame 1 (BALF1), and BALF1 was incorporated into the tegument fraction with BSRF1. Taken together, our results indicate that BSRF1 plays a role in secondary envelopment or virion egress in the cytoplasm, as do its homolog genes.

Original languageEnglish
Article number285
JournalViruses
Volume11
Issue number3
DOIs
Publication statusPublished - 01-03-2019

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Reading Frames
Viruses
Genes
Cytoplasm
Bacterial Artificial Chromosomes
Burkitt Lymphoma
T-Cell Lymphoma
Herpesviridae
Golgi Apparatus
Simplexvirus
Virus Replication
Cytomegalovirus
Human Herpesvirus 4
Natural Killer Cells
Virion
Fluorescent Antibody Technique
B-Lymphocytes

All Science Journal Classification (ASJC) codes

  • Infectious Diseases
  • Virology

Cite this

Yanagi, Y., Abdullah Al Masud, H. M., Watanabe, T., Sato, Y., Goshima, F., Kimura, H., & Murata, T. (2019). Initial characterization of the Epstein–Barr virus BSRF1 gene product. Viruses, 11(3), [285]. https://doi.org/10.3390/v11030285
Yanagi, Yusuke ; Abdullah Al Masud, H. M. ; Watanabe, Takahiro ; Sato, Yoshitaka ; Goshima, Fumi ; Kimura, Hiroshi ; Murata, Takayuki. / Initial characterization of the Epstein–Barr virus BSRF1 gene product. In: Viruses. 2019 ; Vol. 11, No. 3.
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Yanagi, Y, Abdullah Al Masud, HM, Watanabe, T, Sato, Y, Goshima, F, Kimura, H & Murata, T 2019, 'Initial characterization of the Epstein–Barr virus BSRF1 gene product', Viruses, vol. 11, no. 3, 285. https://doi.org/10.3390/v11030285

Initial characterization of the Epstein–Barr virus BSRF1 gene product. / Yanagi, Yusuke; Abdullah Al Masud, H. M.; Watanabe, Takahiro; Sato, Yoshitaka; Goshima, Fumi; Kimura, Hiroshi; Murata, Takayuki.

In: Viruses, Vol. 11, No. 3, 285, 01.03.2019.

Research output: Contribution to journalArticle

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Yanagi Y, Abdullah Al Masud HM, Watanabe T, Sato Y, Goshima F, Kimura H et al. Initial characterization of the Epstein–Barr virus BSRF1 gene product. Viruses. 2019 Mar 1;11(3). 285. https://doi.org/10.3390/v11030285