Integrin-linked kinase is required for epidermal and hair follicle morphogenesis

Katrin Lorenz, Carsten Grashoff, Robert Torka, Takao Sakai, Lutz Langbein, Wilhelm Bloch, Monique Aumailley, Reinhard Fässler

Research output: Contribution to journalArticlepeer-review

94 Citations (Scopus)

Abstract

Integrin-linked kinase (ILK) links integrins to the actin cyto skeleton and is believed to phosphorylate several target proteins. We report that a keratinocyte-restricted deletion of the ILK gene leads to epidermal defects and hair loss. ILK-deficient epidermal keratinocytes exhibited a pronounced integrin-mediated adhesion defect leading to epidermal detachment and blister formation, disruption of the epidermal-dermal basement membrane, and the translocation of proliferating, integrin-expressing keratinocytes to suprabasal epidermal cell layers. The mutant hair follicles were capable of producing hair shaft and inner root sheath cells and contained stem cells and generated proliferating progenitor cells, which were impaired in their downward migration and hence accumulated in the outer root sheath and failed to replenish the hair matrix. In vitro studies with primary ILK-deficient keratinocytes attributed the migration defect to a reduced migration velocity and an impaired stabilization of the leading-edge lamellipodia, which compromised directional and persistent migration. We conclude that ILK plays important roles for epidermis and hair follicle morphogenesis by modulating integrin-mediated adhesion, actin reorganization, and plasma membrane dynamics in keratinocytes.

Original languageEnglish
Pages (from-to)501-513
Number of pages13
JournalJournal of Cell Biology
Volume177
Issue number3
DOIs
Publication statusPublished - 07-05-2007
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Cell Biology

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