Interaction between polyanions and cell nuclei

Mechanism of gelatination of nuclei

Akira Nakashima, Keiji Mori, Susumu Sasaki

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We investigated the mechanism by which polyanions gelatinized nuclei of some mouse lymphocytes and ruptured these cells, The gel produced by the addition of dextran sulfate. (DS) to mouse lymphocyte nuclei was composed of histones (H1, H2A, H2B, H3, and H4), DS, and DNA. Adding DS to the chromatin obtained from nuclei by micrococcal nuclease (MNase) digestion also produced a gel containing a complex of DS-histones-DNA. When this mixture was further digested by MNase, DNA debris of random sizes was observed instead of the 150-bp repeating units of DNA usually observed when normal nucleosomes are digested with MNase. Removal of DS from the chromatin-DS gel resulted in the regeneration of nucleosomes. These results suggest the following: After entering the cells with damaged cellular membrane, DS extracts histones from nucleosomes to form DS-histone complexes, which then aggregate with the liberated DNA to form a macromolecular gel. Finally, the swelling pressure of the gel destroys the cells.

Original languageEnglish
Pages (from-to)846-851
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume228
Issue number3
DOIs
Publication statusPublished - 21-11-1996

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Cell Nucleus
Micrococcal Nuclease
Gels
Histones
Cells
Nucleosomes
DNA
Lymphocytes
Chromatin
Dextran Sulfate
Debris
Swelling
Regeneration
Digestion
polyanions
Membranes
Pressure

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

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abstract = "We investigated the mechanism by which polyanions gelatinized nuclei of some mouse lymphocytes and ruptured these cells, The gel produced by the addition of dextran sulfate. (DS) to mouse lymphocyte nuclei was composed of histones (H1, H2A, H2B, H3, and H4), DS, and DNA. Adding DS to the chromatin obtained from nuclei by micrococcal nuclease (MNase) digestion also produced a gel containing a complex of DS-histones-DNA. When this mixture was further digested by MNase, DNA debris of random sizes was observed instead of the 150-bp repeating units of DNA usually observed when normal nucleosomes are digested with MNase. Removal of DS from the chromatin-DS gel resulted in the regeneration of nucleosomes. These results suggest the following: After entering the cells with damaged cellular membrane, DS extracts histones from nucleosomes to form DS-histone complexes, which then aggregate with the liberated DNA to form a macromolecular gel. Finally, the swelling pressure of the gel destroys the cells.",
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Interaction between polyanions and cell nuclei : Mechanism of gelatination of nuclei. / Nakashima, Akira; Mori, Keiji; Sasaki, Susumu.

In: Biochemical and Biophysical Research Communications, Vol. 228, No. 3, 21.11.1996, p. 846-851.

Research output: Contribution to journalArticle

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T1 - Interaction between polyanions and cell nuclei

T2 - Mechanism of gelatination of nuclei

AU - Nakashima, Akira

AU - Mori, Keiji

AU - Sasaki, Susumu

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AB - We investigated the mechanism by which polyanions gelatinized nuclei of some mouse lymphocytes and ruptured these cells, The gel produced by the addition of dextran sulfate. (DS) to mouse lymphocyte nuclei was composed of histones (H1, H2A, H2B, H3, and H4), DS, and DNA. Adding DS to the chromatin obtained from nuclei by micrococcal nuclease (MNase) digestion also produced a gel containing a complex of DS-histones-DNA. When this mixture was further digested by MNase, DNA debris of random sizes was observed instead of the 150-bp repeating units of DNA usually observed when normal nucleosomes are digested with MNase. Removal of DS from the chromatin-DS gel resulted in the regeneration of nucleosomes. These results suggest the following: After entering the cells with damaged cellular membrane, DS extracts histones from nucleosomes to form DS-histone complexes, which then aggregate with the liberated DNA to form a macromolecular gel. Finally, the swelling pressure of the gel destroys the cells.

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